Cytotoxic and Antimicrobial Activities ofCantharellus cibariusFr. (Cantarellaceae)

Abstract

Antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of Cantharellus cibarius were tested. Broth microdilution assay was performed against 10 bacterial strains (Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus subtilis, Enterococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella abony), with emphasis on Helicobacter pylori. Methanol extract was the most active against H. pylori strains with minimal inhibitory concentration values between 4 and 32 μg/mL. All extracts were active against antibiotic resistant H. pylori. Methanol and aqueous extracts had no cytotoxicity against tested cell lines, whereas cyclohexane and dichloromethane extracts were active against HeLa and N87 cells, but also against healthy MRC-5 cells (IC 39.26 ± 1.24-134.79 ± 0.01 μg/mL). The tested aqueous extracts have shown 68% of angiotensin-converting enzyme inhibitory activity in doses of 1.25 mg/mL. Chemical analysis has shown the presence of linoleic, cis-vaccenic, and oleic acids, sterols, β-glucans, and polyphenolic compounds.

Mineral Composition of Three Popular Wild Mushrooms from Poland

“…Falandysz et al (2017) [ 36 ] also recorded a Cu content of 41 mg/kg d.w. (34–53 mg/kg d.w.) in C. cibarius from Poland, however in these mushrooms from China, it was 31 mg/kg d.w. Smaller amounts of Cu–31.2 mg/kg d.w. were detected by Yildiz et al (2019) [ 28 ] in mushrooms from Turkey, while higher contents were determined by Kolundžić et al (2017) [ 29 ] in C. cibarius from Serbia: 60 mg/kg d.w. Mleczek et al (2016) [ 50 ] observed 18.7 mg/kg d.w. Cu in mushroom species growing near heavily trafficked road in Poland. Cu content in the B. badius ranging from 9.70–13.6 mg/kg d.w. was observed by Mleczek et al (2013) [ 37 ], who were examining fungi from five regions of Poland.…”

“…Comparable manganese contents in C. cibarius were obtained by Ayaz, et al (2011) [ 54 ] 25.2 mg/kg d.w. Brzezicha-Cirocka et al (2016) [ 34 ] determined 30 mg/kg d.w. (20–63 mg/kg d.w.) of this mineral, while Falandysz et al (2017) [ 36 ] detected 38 mg/kg d.w. in C. cibarius from Poland and 19 mg/kg d.w. in this species from China. Yildiz et al (2019) [ 28 ] determined only 4.6 mg/kg of Mn in C. cibarius from Turkey, while Kolundžić et al (2017) [ 29 ] reported up to 41 mg/kg d.w. in this mushroom from Serbia ( Table 5 ). Similar contents of Mn in B. badius from unpolluted areas of the Wielkopolska region, ranging from 11.3 to 14.3 mg/kg d.w. were noted by Mleczek et al (2016) [ 43 ], as well as Karmańska & Wędzisz (2010) [ 42 ] who found 14–17 mg/kg d.w. Kojta & Falandysz (2016) [ 33 ] determined 34.5 mg/kg d.w. in B. badius collected from Kętrzyn and 26.5 mg/kg d.w. in this species from Augustów: Low Mn contents were determined by Mleczek et al (2016) [ 43 ], who were examining B. badius samples from areas where alkaline flotation tailings from Cu production were stored: 1.4–1.8 mg/kg d.w.…”

“…Brzezicha-Cirocka et al (2016) [ 34 ] determined Fe at the level of 170–520 mg/kg d.w. in C. cibarius from Morąg. In this species analyzed in Turkey and Serbia, 588.5 and 234 mg/kg d.w., respectively, were recorded [ 28 , 29 ]. Mleczek et al (2016) [ 43 ] determined a lower Fe content in the B. badius in Wielkopolska region: 24–29 mg/kg d.w. and 28–35 mg/kg d.w. from a polluted area of the province of Lower Silesia.…”

“…Currently, knowledge of the mineral content in edible mushrooms is relatively extensive. In many countries (South Africa, Turkey, Greece, Serbia, China, and others), numerous studies have been conducted on the mineral composition of various species of fungi to more accurately estimate their nutritional and health value and the mechanism of accumulation of individual components [ 22 , 27 , 28 , 29 , 30 , 31 , 32 ]. In Poland, large-scale analyses have been conducted, among others, by Falandysz, whose numerous reports concern a wide spectrum of elements determined in many species of mushrooms collected in various sites in Poland and around the world [ 33 , 34 , 35 , 36 ].…”

Medicinal potential of extracts from the chanterelle mushroom, Cantharellus cibarius (Review) and prospects for studying its strains from differs plant communities of ultracontinental regions of the Asia

“…All extracts were active against antibiotic resistant H. pylori. Chemical analysis has shown the presence of linoleic, cis-vaccenic, and oleic acids, sterols, β-glucans, and polyphenolic compounds [19].…”