Abstract:We evaluated the suitability of 2% human platelet lysate medium (2%HPL) as a replacement for 2% fetal bovine serum medium (2%FBS) for the xeno-free organ culture of human donor corneas. A total of 32 corneas from 16 human donors were cultured in 2%FBS for 3 days (TP1), then evaluated using phase contrast microscopy (endothelial cell density (ECD) and cell morphology). Following an additional 25-day culture period (TP2) in either 2%FBS or 2%HPL, the pairs were again compared using microscopy; then stroma and De… Show more
“…Talpan et al, presented an interesting report on the use of human platelet lysate (HPL) as a xeno-free substitute for fetal bovine serum (FBS) in human donor cornea organ cultures [15]. Xenogenic FBS is habitually used during the storage and transport of donor corneal tissue, as well as during the preparation of cell-based and bioengineered corneal therapy approaches.…”
“…Talpan et al, presented an interesting report on the use of human platelet lysate (HPL) as a xeno-free substitute for fetal bovine serum (FBS) in human donor cornea organ cultures [15]. Xenogenic FBS is habitually used during the storage and transport of donor corneal tissue, as well as during the preparation of cell-based and bioengineered corneal therapy approaches.…”
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