Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture

Plotnikov, Egor Y.; Khryapenkova, T. G.; Galkina, Svetlana I.; Сухих, Г. Т.; Zorov, Dmitry B.

doi:10.1016/j.yexcr.2010.06.009

Cited by 143 publications

(113 citation statements)

References 20 publications

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“…The asymmetrical diagrams may reflect combined effect of various properties of DiO and DiD and reveals more efficient flow of both fluorescent lipophilic dyes DiO and DiD from MSCs toward NPCs in the direct systems (25). Asymmetrical secretion of lipophylic dye DiO was already observed in co-culture of renal tubular cells and MSCs (26). In the indirect system we did not observe this preferred direction of the DiD flow from MSCs toward NPCs.…”

Section: Discussionmentioning

confidence: 67%

Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Lehmann

Filipiak

Juzwa

et al. 2013

Molecular Medicine Reports

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Abstract. Degeneration of the intervertebral disc (IVD) is the main cause of age-related damage of spinal tissues. Using multipotent mesenchymal stromal cells (MSCs) regenerative medicine intends to restore the IVD components of annulus fibrosus (AF) and nucleus pulposus (NP). In the present study NP cells (NPCs) and MSCs obtained from adolescent patients suffering from scoliosis were used. IVDs and vertebrae were obtained during surgery and subsequently processed in order to establish cultures of NPCs and MSCs. The two cell types were co-cultured in 1-µm pore size insert system (indirect co-culture) or on one surface (direct co-culture). Prior to co-culture in these systems one of the cell types was stained by lipophilic fluorescent dye DiD (red). The results demonstrated that regardless of the cell type, the flow of DiD from stained to non-stained cells was more efficient in the direct co-culture in comparison with the insert system. Moreover, in the direct system the DiD flow was more efficient from MSCs towards NPCs compared with that in the opposite direction. These data indicated that the membrane interchange between the two cell types was asymmetric. To discriminate the subpopulation of cells that underwent membrane interchange, cells were double stained with DiD and DiO (green). In the first part of the experiment NPCs were stained by DiO and MSCs by DiD. In the second, NPCs were stained by DiD and MSCs by DiO. The cells were co-cultured in the direct system for 8 days and subsequently analyzed by flow cytometry and confocal microscopy.This analysis revealed that >50% of cells were stained by the DiO and DiD dyes. NPCs and MSCs formed structures similar to tunnelling nanotubes (TnT). In conclusion, the formation of TnT-like structures is able to promote, phenotypic changes during the direct co-culture of NPCs with MSCs.

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Section: Discussionmentioning

confidence: 67%

Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Lehmann

Filipiak

Juzwa

et al. 2013

Molecular Medicine Reports

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“…The molecules in MNTs are transferred bi-or unidirectionally, probably depending on the cell type. Cellular components are transferred efficiently from rat renal tubular cells to bone-marrow multipotent MSCs [29]. Mitochondria could be transported via MNTs between progenitor and endothelial cells and between cardiac myocytes and cardiac fibroblasts [5,30].…”

Section: Transport Of Organelles and Signalsmentioning

confidence: 99%

Membrane nanotubes: Novel communication between distant cells

Zhang

2013

Sci. China Life Sci.

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The many kinds of cell structures involved in cell-cell communication include tight junction, adherens junction and gap junction, but almost all are between adjacent cells. Recently, a general and dynamic membrane tether, termed tunneling nanotubes or membrane nanotubes (MNTs), was discovered to be involved in communication between distant cells. By facilitating intercellular communication, MNTs contribute to many biological functions and pathologic changes in cells. Many works have revealed the structure, formation and functional properties of MNTs. However, as novel structures, further research is needed.

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“…This suggests the existence of some paracrine mechanisms resulting from cell-to-cell interactions. Recently, the possibility of cell-to-cell crosstalk with transfer of cytoplasm [12][13][14] or mitochondria [14][15][16][17] was demonstrated for different types of stem and differentiated cells. For neurons and MMSCs, such interactions have been poorly explored, although the cell-to-cell transfer of cell contents could have a critical role for the physiology of both neurons and MMSCs.…”

Section: Introductionmentioning

confidence: 99%

Improving the Post-Stroke Therapeutic Potency of Mesenchymal Multipotent Stromal Cells by Cocultivation With Cortical Neurons: The Role of Crosstalk Between Cells

Babenko

Silachev

Zorova

et al. 2015

Stem Cells Translational Medicine

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The goal of the present study was to maximally alleviate the negative impact of stroke by increasing the therapeutic potency of injected mesenchymal multipotent stromal cells (MMSCs). To pursue this goal, the intercellular communications of MMSCs and neuronal cells were studied in vitro. As a result of cocultivation of MMSCs and rat cortical neurons, we proved the existence of intercellular contacts providing transfer of cellular contents from one cell to another. We present evidence of intercellular exchange with fluorescent probes specifically occupied by cytosol with preferential transfer from neurons toward MMSCs. In contrast, we observed a reversed transfer of mitochondria (from MMSCs to neural cells). Intravenous injection of MMSCs in a postischemic period alleviated the pathological indexes of a stroke, expressed as a lower infarct volume in the brain and partial restoration of neurological status. Also, MMSCs after cocultivation with neurons demonstrated more profound neuroprotective effects than did unprimed MMSCs. The production of the brain-derived neurotrophic factor was slightly increased in MMSCs, and the factor itself was redistributed in these cells after cocultivation. The level of Miro1 responsible for intercellular traffic of mitochondria was increased in MMSCs after cocultivation. We conclude that the exchange by cellular compartments between neural and stem cells improves MMSCs' protective abilities for better rehabilitation after stroke. This could be used as an approach to enhance the therapeutic benefits of stem cell therapy to the damaged brain. STEM CELLS

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Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture

Cited by 143 publications

References 20 publications

Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Membrane nanotubes: Novel communication between distant cells

Improving the Post-Stroke Therapeutic Potency of Mesenchymal Multipotent Stromal Cells by Cocultivation With Cortical Neurons: The Role of Crosstalk Between Cells

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