Cytometric, morphologic and enzymatic characterisation of haemocytes in Anodonta cygnea

Soares-da-Silva, I.M; Ribeiro, Danilo Monteiro; Valongo, Carla; Pinto, Rui; Vilanova, Manuel; Bleher, Reiner; Machado, Jorge

doi:10.1016/s1095-6433(02)00039-9

Cited by 43 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, since haemocytes are involved in processes such as the elimination of toxic substances and small particles, transport and digestion of nutrients, and repair of tissue lesions [Soares-da-Silva et al, 2002, and references therein], they represent cells of relevant importance in the mussel's response to toxicants. Haemolymph readily provides a single cell suspension; it is easily collectable and its usage permits repeatable tissue sampling of the same individuals.…”

Section: Introductionmentioning

confidence: 99%

Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide

Štambuk

Pavlica

Malović

et al. 2008

Environ and Mol Mutagen

View full text Add to dashboard Cite

An important endpoint in assessing pollution-related toxicity is genotoxicity. To obtain insight into the time-course of oxidative- and alkylation-induced DNA damage in the freshwater mussel, Unio pictorum, mussels were exposed for 24 hr to concentration gradients of pro-oxidant hydrogen peroxide (H(2)O(2)) and a mono-functional alkylating agent, ethyl methanesulfonate (EMS). DNA damage was assessed in haemocytes immediately upon exposure and over the recovery period of up to 72 days by means of comet and micronucleus assays. Following exposure to H(2)O(2), DNA damage as detected by the comet assay returned to control values after one day, except for the mussels exposed to the highest dose when damage was detectable for the next 3 days. In contrast, alkylation-induced DNA damage was detectable even after 72 days of recovery in de-chlorinated water, with a dose-response relationship observable throughout the whole recovery period. Micronucleus frequency was the highest on Day 3 after exposure to EMS; it decreased considerably by Day 7 and returned almost to the control levels 19 days after exposure, while no significant induction of micronuclei was observed in mussels exposed to H(2)O(2). Although the comet assay is considered a biomarker of recent genotoxic exposure, detecting DNA damage of shorter longevity than with the micronucleus assay, results presented here show that in the case of alkylation damage the comet assay reveals genotoxic exposure of U. pictorum in a dose-dependent manner even after 2 months.

show abstract

Section: Introductionmentioning

confidence: 99%

Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide

Štambuk

Pavlica

Malović

et al. 2008

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Hemocytes (Fig. 2) have a role in processes such as the transport and digestion of nutrients and elimination of toxic substances and small particles, which makes them constantly exposed to waterborne pollutants [23,34,35]. Hemolymph can be easily collected from the adductor muscle and, most importantly, collecting does not require sacrificing animals (Fig.…”

Section: Freshwater Mussels As Bioindicatorsmentioning

confidence: 99%

Genotoxicological Studies of Lower Stretch of the Sava River

Vuković-Gačić

Kolarević

Sunjog

et al. 2014

The Handbook of Environmental Chemistry

View full text Add to dashboard Cite

Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.

show abstract

“…This enzyme not only converts malate to oxaloacetate but also plays a significant role in CO (Rodriguez, Esteban, & Meseguer, 2003;Soares-da-Silva et al, 2002), cell adhesion (Holmb lad & Soderhall, 1999), antio xidant function (Gamble, Go ldfarb, Porte, & Livingstone, 1995;Galloway & Depledge, 2001) and helps in formation of melan in by oxidative polymerizat ion of hydroquinones (D'Ischa, Napolitano, & Prota, 1991). Previous investigations confirmed that due to pesticidal impact on fish reveals changes in protein profile.…”

Section: Introductionmentioning

confidence: 98%

Untitled

Choudhury¹,

Tarafdar²,

Panigrahi³

2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The present investigation is about change in antioxidant enzymes and protein profile in Oreochromis mossambicus and Channa punctatus due to toxicity induced by application of fungicides in paddy -cum-fish ecosystem in India. Antioxidant enzymes like Lactate dehydrogenase (LDH), M alate dehydrogenase (MDH) and Peroxidase (Pox) are used as biomarkers for toxicological study. Oreochromis mossambicus and Channa punctatus were exposed to different concentration of Azoxystrobin 23% SC and Hexaconazole 5% SC respectively and LC 50 was determined. LC 50 for Amister was detected to be 0.008 ml/lit and that for Contaf plus was found to be 0.025 ml/lit. In case of Oreochromis mossambicus 1/2.5 th , 1/5 th and 1/7.5 th of LC 50 (0.0032 ml/lit, 0.0016 ml/lit and 0.001 ml/lit) were selected for chronic sub-lethal study. For Channa punctatus ½ nd , ¼ th and 1/6 th of LC 50 value (0.0125 ml/lit, 0.0062 ml/lit and 0.0041 ml/lit) were chosen. After 3 months of exposure, the individuals exposed to toxicity were sacrificed and stress enzymes and protein profile were checked in gill, heart, liver, kidney, muscle and spleen. Overall result revealed a gradual incr ease in LDH and Peroxidase but decrement in level of M DH and protein profile was also changed due to toxicity by fungicides.

show abstract

Cytometric, morphologic and enzymatic characterisation of haemocytes in Anodonta cygnea

Cited by 43 publications

References 32 publications

Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide

Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide

Genotoxicological Studies of Lower Stretch of the Sava River

Untitled

Contact Info

Product

Resources

About