Cytological Effects of Different Mutagens in Lentil(Lens culinaris Medik).

Reddy, V. R. K.; Annadurai, M.

doi:10.1508/cytologia.57.213

Cited by 18 publications

(8 citation statements)

References 4 publications

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“…Combination treatments recorded the highest frequency of pollen sterility followed by MMS and gamma rays. These results are in agreement with many workers (Nerker 1970, Bhamburker and Bhalla 1985, Reddy and Annadurai 1992 who have also reported dose dependent increase in pollen sterility following mutagenic treatments. The high pollen sterility observed in the treated populations may be attributed to the vast array of meiotic aberrations that were induced by mutagens leading to the formation of aberrant pollen grains (Rana andSwaminathan 1964, Sinha andGodward 1972).…”

Section: Resultssupporting

confidence: 93%

“…Different types of meiotic abnormalities as observed in the present investigation have also been reported by different workers in different plant materials after treatment with physical and chemical mutagens (Reddy and Annadurai 1992, Mitra and Bhowmik 1996, Kumar and Srivastava 2001, Sharma and Kumar 2004. The analysis of various meiotic abnormalities at different stages of cell division clearly demonstrated that combination treatments were more effective in inducing the maximum frequency of chromosomal aberrations followed by individual treatments of MMS and 2009 gamma rays.…”

Section: Resultssupporting

confidence: 86%

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Assessment of Mutagenicity of Individual and Combination Treatments of Gamma Rays and MMS in Broad Bean (Vicia faba L.)

2009

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Summary Seeds of Vicia faba L. var. PRT-12 were subjected to different doses/concentrations of gamma rays and methyl methane sulphonate (MMS) individually as well as in combination. The effect of different mutagenic treatments on meiosis and pollen fertility has been studied in M 1 generation. Various types of meiotic aberrations and reduction in pollen fertility were observed in all the treatments. However, the combination treatments proved to be more effective in inducing meiotic aberrations and reduction in pollen fertility as compared to individual ones. Moreover, the frequency of meiotic aberrations was at its maximum at metaphase followed by anaphase and telophase stages.Key words Vicia faba L., Gamma rays, MMS, Meiotic aberrations, Pollen.Cytogenetical investigation is an important source of information regarding genetical hazards due to chemical and physical mutagens. The most common factor of mutagen-induced sterilily is chromosomal aberrations and hence the most common form of sterility is the occurence of nonfunctional gametes. Cytogenetic evidence is one of the most documented experimental proofs for the elucidation of the mode of speciation on various genotypes to a particular mutagen.Vicia faba L. (2nϭ12), commonly known as the broad bean, is a leguminous crop belonging to the family Fabaceae. It is an important pulse crop used as a vegetable, silage, forage and stock feed. Since the genotype of V. faba L. is homozygous because of its being self-pollinated leading to little progress in crop improvement, it needs induction of variations to facilitate genetic improvement by mutation breeding. In the present investigation, attempts have been made to explore the possibilities of inducing alterations in the genotype to enhance the genetic variability through the use of physical and chemical mutagens, i.e., gamma rays and MMS, individually as well as in combination. Materials and methodsTwo hundred healthy, pure and dry seeds (10-12% moisture content) of V. faba L. var. PRT-12 were used for each treatment of gamma rays, MMS and combination of both, along with control. Seeds were irradiated with 4 doses of gamma rays viz., 5, 10, 15 and 20 kR from Co 60 source at NBRI, Lucknow. For chemical mutagenic treatments, seeds were first presoaked in distilled water for 12 h and then treated with different concentrations (0.01, 0.02, 0.03 and 0.04%) of freshly prepared aqueous solution of MMS in phosphate buffer (pH 7.0) for 6 h. For combination treatments, seeds irradiated with 10 kR dose of gamma rays were presoaked in distilled water for 12 h and then treated with all the concentrations of MMS for 6 h in the same manner as described earlier. After the treatment period was over, the mutagen treated seeds were thoroughly washed in running tap water for about 1 h to remove the residual effect of chemical, if any.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 86%

Assessment of Mutagenicity of Individual and Combination Treatments of Gamma Rays and MMS in Broad Bean (Vicia faba L.)

2009

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“…It is, thus, noticeable that as more and more abnormalities accumulate, the process of gamete formation is affected and it will leads to the formation of non-viable gametes that could considerably reduce the plant fertility (Kumar and Rai 2007). These results are in agreement with many workers (Nerker 1970;Bhamburker and Bhalla 1985;Reddy and Annadurai 1992;Khan et al 2009), who have also reported dose dependent increase in pollen sterility following mutagenic treatments.…”

Section: Resultssupporting

confidence: 93%

Comparative genotoxic potential of <i>γ</i> rays in two varieties of Isabgol (<i>Plantago ovata</i> Forsk.)

Mishra

Khan

2015

Chromosome Botany

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The present investigation deals with the mutagenic effects of six doses of γ rays viz., 15 kR, 30 kR, 45 kR, 60 kR, 75kR and 90 kR on pollen fertility and meiosis in M 1 generation of two varieties of Isabgol (Plantago ovata Forsk.) i.e., var. Mayuri and var. Niharika. These studies were carried out in control as well as in treated population of both the varieties. During the meiotic studies various types of chromosome aberrations were identified at different stages of meiosis in all the doses of γ rays. These anomalies were observed to be dose dependent and positively correlated with the doses of γ rays applied in both the varieties. The frequency of meiotic aberrations was maximum at metaphase followed by anaphase and telophase stages. Pollen fertility had also shown a gradual decrease with an increase in doses of γ rays in both the varieties. These collective observations had indicated that γ rays are more effective in var. Mayuri as it produced more chromosomal aberrations in var. Mayuri in comparison with var. Niharika, and equally suggested that var. Mayuri is more sensitive to the γ rays than the var. Niharika.

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“…Higher percentages of multivalents and anaphase-I bridges were recorded in the present study as compared to other types of meiotic abnormalities. Reddy and Annadurai (1992) also reported a dose dependent increase in the number of quadrivalents in Lens culinaris. Bridge formation can be attributed to the general stickiness of the chromosomes at mataphase stage or breakage and reunion of chromosomes.…”

Section: Discussionmentioning

confidence: 85%

Meiotic Studies in Two Cultivars of Cicer arietinum L. after Gamma Irradiation.

Ahmad

1993

CYTOLOGIA

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Cytological Effects of Different Mutagens in Lentil(Lens culinaris Medik).

Cited by 18 publications

References 4 publications

Assessment of Mutagenicity of Individual and Combination Treatments of Gamma Rays and MMS in Broad Bean (Vicia faba L.)

Assessment of Mutagenicity of Individual and Combination Treatments of Gamma Rays and MMS in Broad Bean (Vicia faba L.)

Comparative genotoxic potential of <i>γ</i> rays in two varieties of Isabgol (<i>Plantago ovata</i> Forsk.)

Meiotic Studies in Two Cultivars of Cicer arietinum L. after Gamma Irradiation.

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