Cytokine profile in Egyptian hepatitis C virus genotype-4 in relation to liver disease progression

Zekri, Abdel-Rahman N.; Ashour, M. S. E.; Hassan, Ahmed; El-Din, Hanaa M Alam; El-Shehaby, Amal; Abu-Shady, Maha A

doi:10.3748/wjg.v11.i42.6624

Cited by 55 publications

(57 citation statements)

References 30 publications

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“…Interestingly, our data demonstrated an increased frequency of IL-6, IL-10 and IL-12 producers in RIBA-POS blood donors, similar to that observed in the viremic group. The increased frequency of high IL-6 and IL-10 producers in these subjects is similar to reports examining chronically infected HCV patients [33,34] . Since cytokine genes are polymorphic, and certain mutations located within coding/regulatory regions have been shown to affect the overall expression and secretion of cytokines, there may be a correlation between cytokine gene polymorphisms and their association with the outcome of HCV infection.…”

Section: Discussionsupporting

confidence: 75%

IL-8 Is Associated with Non-Viremic State and IFN-γ with Biochemical Activity in HCV-Seropositive Blood Donors

et al. 2010

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Objective: The aim of this study was to explore a possible association between the pattern of serum cytokines with the virological and biochemical status of hepatitis C virus (HCV)-seropositive blood donors. Methods: 23 non-viremic and 33 viremic HCV-seropositive blood donors based on HCV-RNA tests, and 29 healthy individuals were included. Cytometric bead array assays were performed to detect cytokines. Results: The subjects were classified as low, medium or high cytokine producers based on the tertile distribution. The absence of detectable viremia was associated with high IL-1β and IL-8 producers. Conversely, elevated levels of IL-6, IL-10 and IL-12 were associated with detectable viremia. An increased frequency of high IL-1β producers was observed frequently in the non-viremic recombinant immunoblot assay (RIBA)-indeterminate subjects, while the high IL-4, IL-6, IL-8, IL-10 and IL-12 producers were more frequent in the non-viremic RIBA-positive subjects. Furthermore, the levels of IL-1β and IL-8 were higher in viremic subjects with a low level of alanine-aminotransferase (ALT), whereas the level of IFN-γ was increased among viremic subjects with a high ALT level. Conclusion: IL-1β and IL-8 were more likely to be associated with a non-viremic or less severe HCV infection, whereas IL-2 and IFN-γ levels correlated with a high ALT level.

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Section: Discussionsupporting

confidence: 75%

IL-8 Is Associated with Non-Viremic State and IFN-γ with Biochemical Activity in HCV-Seropositive Blood Donors

et al. 2010

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“…In addition, the levels of the TNF-αRs, TNF-αRI and TNF-αRII, were higher in HCC patients when compared with healthy individuals (162). However, in other studies, TNF-α levels were lower in HCC tumor tissue versus the tissue surrounding the tumor and in HCC patients versus healthy individuals (163,164). In a study by Kakumu et al TNF-αRs correlated with disease progression and IFN-α treatment did not affect their level (162).…”

Section: Hepatic Stellate Cells (Hscs)mentioning

confidence: 90%

“…Several groups have shown that IL-10 is highly expressed in HCC tumors and individuals with HCC versus non-tumorous-surrounding tissue controls or healthy cohorts (162,164,(173)(174)(175). These studies suggest that increases in IL-10 and perhaps other Th2 cytokines correlate with progression.…”

Section: Hepatic Stellate Cells (Hscs)mentioning

confidence: 97%

The tumor microenvironment in hepatocellular carcinoma (Review)

et al. 2012

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Abstract. The tumor microenvironment has been largely studied as a dynamic system orchestrated by inflammatory cells, including cancer cells, stroma as well as the extracellular matrix. It is useful to describe and predict the phenotypic characteristics of cancer. Furthermore, a better understanding of its interplay with the various aspects of the tumor cells may be utilized for the discovery of novel molecular targets. Liver cancer is considered a model of the relation occurring between the tumor microenvironment and tumor development. The chronic inflammatory status of the liver, sustained by the infection of hepatitis viruses, as well as the production of cytokines and growth factors within the parenchyma, lead to an intricate microenvironment. The identification of novel molecular therapeutic targets may improve the outcome of patients with liver cancer as it remains the third leading cause of cancer death worldwide. In the present study, the tumor microenvironment in hepatocellular carcinoma (HCC) was explored by a review of the literature. Studies on hepatitis virus infections and the consequent chronic inflammatory status were examined. In this context, immune-mediated and/or virusrelated molecular mechanisms have been hypothesized as being responsible for liver cancer development. The interlink among HCC microenvironment components, comprising cellular elements, cytokines, growth factors and several proteins is also described together with the role of matrix metalloproteinases in HCC development. Finally, the rationale for targeting tumorstromal interface is summarized in the context of new therapeutic opportunities.

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“…Differences among HCV genotypes in geographic distributions have provided investigators with an epidemiologic marker that can be used to trace the source of HCV infection in a given population 5 . Hepatitis C virus genotypes and subtypes determination is an important factor in the pretreatment evaluation of the patients and in defining better treatment strategies 18 .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of hepatitis C virus in multi renal dialysis centers in Egypt

2013

Al-Azhar University Journal of Virus Researches and Studies

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Hepatitis C virus (HCV) is a positive-stranded RNA virus, there is at least six major genotypes many of which contain a number of more closely related subtypes Objectives: Two HCV genotyping method were used to study the genotypic distribution of HCV among renal dialysis patients.Acute and chronic HCV infection was diagnosed among patients undergoing hemodialysis. Anti-HCV ELISA was performed on the blood samples of the cases and sample found in cutoff value were tested for presence of HCV-RNA. In this study genotyping for HCV infected patients was based on sequence analysis of 5' untranslated region (5'UTR) and using type specific primers of core regions HCV-RNA was detected by RT-PCR in 50 patients. HCV-RNA was detected in 19 /20 (95%) seroconverted patients. The thirty patient cannot be diagnosis by third generation ELISA (Abs of sample / cut off =1) and need confirmatory test by PCR. During this study, HCV -RNA were detected in 25/30 (83.3%), while HCV -RNA were negative in 16.6% . It was observed that none of the patients (34 patients positive for HCV-RNA) were infected with HCV Genotype 1 by using type specific primer method. 20 out of thirty four patient genotyping by type specific primer method were genotyping by direct sequencer. Genotype 4a was detected in 15 patients out of 20. Additionally, 5 patients could not be genotype by direct sequencer; their reliability may be referring to DNA lost during storage or due to improper laboratory handling. The immunocompromised state of hemodialysis(HD) patients is usually regarded as an explanation for their deficient antibody response to HCV virus. In our study we demonstrated that ELISA test is not enough for diagnosis HCV in renal dialysis patients for this reason. Genotype 4a is the most common genotype in Egypt. Sequence analysis is reference method for genotyping HCV Introduction The term genotype refers to different genetic variations or strains of hepatitis C. HCV constantly changes and mutates as it replicates more than 1 trillion hepatitis C virions replicate each day. Diagnosis of this infection has evolved remarkably, progressing from the simple detection of anti-HCV antibodies by ELISA to molecular methods 1 .With early serological assays, especially

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Cytokine profile in Egyptian hepatitis C virus genotype-4 in relation to liver disease progression

Abstract: Liver disease progression from CLD to HCC due to HCV genotype-4 infection is associated with an imbalance between Th1 and Th2 cytokines. IL-2R, TNF-RI, and TNF-RII could be used as potential markers.

Cited by 55 publications

References 30 publications

IL-8 Is Associated with Non-Viremic State and IFN-γ with Biochemical Activity in HCV-Seropositive Blood Donors

IL-8 Is Associated with Non-Viremic State and IFN-γ with Biochemical Activity in HCV-Seropositive Blood Donors

The tumor microenvironment in hepatocellular carcinoma (Review)

Genetic diversity of hepatitis C virus in multi renal dialysis centers in Egypt

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