Cytogenetic Consequences of the Mixed Effects of Opistorchiasis and Tick-Borned Encephalitis in Association With Polymorphism by Gluthathione-S-Transferase Genes

Abstract

Purpose: to study the duration of the preservation of the cytogenetic effects of tickborne encephalitis (TBE) in individuals infected with the Opisthorchis felineus (OF) helminth, which differ in alleles of the glutathione-S-transferase enzyme genes. Methods: the objects of the study were patients with tick-borne encephalitis in whom the frequencies of micronucleated lymphocytes were detected. All examined patients were subdivided into two groups: TBE patients non-infected with OF, and TBE patients infected with OF. Multiplex PCR was used to analyze the GSTM1 and GSTT1 genes. Results: patients with TBE, infected with OF, had a significantly lower increase in the level of cytogenetic disorders than patients with TBE without OF infection; at the same time, the restoration of the cytogenetic norm in this group was not reached, and there was no association between the frequency of micronucleated lymphocytes and different variants of the glutathione-S-transferase enzyme genes. Conclusion: the frequency of cytogenetic changes in TBE patients infected with OF are significantly less than one in patients with TBE without OF infection. Polymorphism in the genes of the enzyme glutathione-S-transferase does not have significant effect on the cytogenetic disorders in patients with TBE with OF infection.