Cytogenetic and molecular genetic methods for diagnosis and treatment response in chronic granulocytic leukemia

Dewald, Gordon W.; Juneau, Amy L.; Schad, Christopher R.; Tefferi, Ayalew

doi:10.1016/s0165-4608(96)00292-0

Cited by 27 publications

(8 citation statements)

References 48 publications

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“…20 The high value of FISH analysis with ABL and BCR probes in the initial diagnosis and for the detection of residual disease in CML patients has been demonstrated in several studies. [14][15][16][21][22][23][24][25][26][27][28] In ALL, the applicability of FISH to detect the BCR/ABL rearrangement has only been addressed in small series of selected patients and in single cases with variant Ph translocations. 15,16,20,29,30 Using FISH on interphase nuclei, a colocalization of a BCR and ABL hybridization spot may occur by chance due to the two-dimensional projection of the BCR and ABL hybridization signals of the three-dimensional nucleus, and thus lead to a false positive result.…”

Section: Introductionmentioning

confidence: 99%

High rate of chromosome abnormalities detected by fluorescence in situ hybridization using BCR and ABL probes in adult acute lymphoblastic leukemia

et al. 1998

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8 GMALL study groupThe value of dual-color fluorescence in situ hybridization (FISH) with BCR and ABL probes for the detection of the Philadelphia (Ph) translocation and of other alterations involving ABL and/or BCR was evaluated in adult acute lymphoblastic leukemia (ALL). One hundred and four patients were studied prospectively using interphase nuclei FISH, chromosome analysis (CA), and PCR assays for the chimeric BRC/ABL transcript.

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Section: Introductionmentioning

confidence: 99%

High rate of chromosome abnormalities detected by fluorescence in situ hybridization using BCR and ABL probes in adult acute lymphoblastic leukemia

et al. 1998

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“…This well‐standardized approach is certainly stressful for patients, costly and time‐consuming. The precision of BM analysis is to quantify that the disease has improved using BM‐FISH analysis (Chen et al ., 1997; Dewald et al ., 1997). Moreover, searching for a less invasive technique than BM, FISH was performed on PB samples and results were compared with cytogenetic findings of the BM.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of interphase fluorescence in situ hybridization for BCR/ABL on peripheral blood smears for monitoring of CML patients: a comparison with bone marrow findings

Akel

Kοlialexi

Mavrou

et al. 2002

Clinical &Amp; Laboratory Haematology

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Conventional cytogenetic analysis (CCA) is the standard method for monitoring of the Philadelphia (Ph) chromosome in chronic myeloid leukemia (CML). Evaluation of breakpoint cluster region/abelson murine leukemia (BCR/ABL) fusion using interphase fluorescence in situ hybridization on peripheral blood smears (PB-FISH) might be another approach allowing more frequent and less invasive follow-up investigations. Herein, BCR/ABL fusion gene was assessed on 21 PB smears from 16 CML patients in chronic phase. Results of PB-FISH were compared with those of CCA and interphase FISH on bone marrow aspirates (BM-FISH). PB-FISH analysis was combined with CD3 immunophenotyping that allowed simultaneous investigation of the leukemic status of CD3(+) T lymphocytes and scoring CD3(-) cells for BCR/ABL fusion gene. Moreover, the frequency of BCR/ABL fusion in nonlymphoid PB cells was estimated according to the differential leukocyte counts. The incidence of BCR/ABL(+) fusion signals in CD3(+) T cells of CML patients was 5.3% (SD +/- 1.9) and did not exceed the normal cut-off value of 8%. A significant correlation (P < 0.001) was found between results of PB-FISH and methods of BM analysis (CCA or BM-FISH). Correction of PB-FISH results to include only nonlymphoid or CD3(-) cells reduced the mean of differences and improved agreement between PB-FISH and CCA or BM-FISH methods. The best agreement was noted between CCA and PB-FISH on nonlymphoid cells. On the other hand, results of BM-FISH agreed well with those of PB-FISH on CD3(-) cells. These findings imply that PB-FISH on nonlymphoid or CD3(-) cells is reliable and may replace BM analysis for monitoring of response to treatment in CML patients.

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“…Generally cytogenetic studies are based on analysis of up to 25 metaphases and can be used to detect most chromosome anomalies and determine the percentage of proliferating neoplastic cells. To accurately 9 luantify proliferating disease, it is important to randomly select metaphases because bias can influence the percentage of normal and abnormal metaphases [7]. Conventional chromosome studies are particularly valuable when a specific hematological diagnosis or classification is not apparent by morphologic criteria.…”

Section: Conventional Cytogenetic Studies Versus Fisil Studiesmentioning

confidence: 99%

Cytogenetic and FISH studies in myelodysplasia, acute myeloid leukemia, chronic lymphocytic leukemia and lymphoma

Dewald

2002

Int J Hematol

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Conventional cytogenetic studies are widely used today to diagnose and manage patients with hematological malignancies. The application of fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes helps to further define molecular subclasses and cytogenetic risk categories for patients with these disorders. Moreover, FISH permits analysis of proliferating (metaphase cells) and non-proliferating (interphase nuclei) cells, and is useful in establishing the percentage of neoplastic cells before and after therapy (minimal residual disease). For patients with myelodysplasia or acute myeloid leukemia, these chromosome techniques are important for accurate diagnosis and classification of disease and to help decide treatment and monitor response to therapy. Conventional cytogenetic studies have been problematic in chronic lymphocytic leukemia because the neoplastic cells divide infrequently. However, interphase FISH studies now permit detection of chromosome anomalies with prognostic significance in chronic lymphocytic leukemia. The World Health Organization recognizes that genetic anomalies are one of the most reliable criteria for classification of malignant lymphomas. New methods to extract individual nuclei from paraffin-embedded tissue are now available which permit the use of interphase FISH to detect important chromosome anomalies in lymphoma.

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Cytogenetic and molecular genetic methods for diagnosis and treatment response in chronic granulocytic leukemia

Cited by 27 publications

References 48 publications

High rate of chromosome abnormalities detected by fluorescence in situ hybridization using BCR and ABL probes in adult acute lymphoblastic leukemia

High rate of chromosome abnormalities detected by fluorescence in situ hybridization using BCR and ABL probes in adult acute lymphoblastic leukemia

Efficiency of interphase fluorescence in situ hybridization for BCR/ABL on peripheral blood smears for monitoring of CML patients: a comparison with bone marrow findings

Cytogenetic and FISH studies in myelodysplasia, acute myeloid leukemia, chronic lymphocytic leukemia and lymphoma

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