Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization

Abstract

The new salt tolerant cereal, Tritipyrum (2n=6x=42, AABBE b E b ) offers potential to introduce desirable characters for wheat improvements. This study was aimed to generate a segregating population from Iranian local wheat cultivars (2n=6x=42, AABBDD) and Tritipyrum crosses, study of the meiotic behaviour in F 2 hybrids and identification of E b chromosomes in F 3 individuals. Results showed meiotic abnormalities in F 2 plants and different pairing frequency in the meiosis among F 2 plants. Genomic in situ hybridization revealed that total and E b chromosome number of F 3 seeds ranged from 39 to 45 and 0 to 10, respectively. A significant prevalence of hyper-aneuploidy was observed among F 3 genotypes. C-banding patterns identified E b chromosomes in Tritipyrum, indicating that it also can be useful to study wheat-Tritipyrum derivatives.

Nucleotide polymorphism pattern and multiple maternal origin in Thinopyrum intermedium inferred by trnH-psbA sequences

“…The ongoing genetic advancement of crops is dependent on a continuing supply of genetic variability. The tribe Triticeae in Poaceae offers a vast gene pool from which traits of agronomic interest can be extracted to enrich the genetic diversity of wheat for disease resistance and quality improvement (Maxted et al 2008, Mirzaghaderi et al 2010. Thinopyrum intermedium (Host) Barkworth & D.R.…”

Nucleotide polymorphism pattern and multiple maternal origin in Thinopyrum intermedium inferred by trnH-psbA sequences

“…The ongoing genetic advancement of crops is dependent on a continuing supply of genetic variability. The tribe Triticeae in Poaceae offers a vast gene pool from which traits of agronomic interest can be extracted to enrich the genetic diversity of wheat for disease resistance and quality improvement (Maxted et al 2008, Mirzaghaderi et al 2010. Thinopyrum intermedium (Host) Barkworth & D.R.…”

Karyology of Iranian Endemic <i>Satureja</i> (Lamiaceae) Species

“…Multivariate statistical analysis (Srivastava 2002) was performed on standardized data (mean=0, variance=1) using the Minitab 16 statistical software (Ryan and Joiner 2001). To evaluate the contribution of each chromosomal parameter to the ordination of genotypes, they were also subjected to principal components analysis (PCA) based on data matrix (Mirzaghaderi et al 2010). Cluster analysis was carried out to examine chromosomal similarity among the genotypes.…”