Cytocompatibility of filling pastes by primary teeth root simulating model

Pintor, Andréa Vaz Braga; Queiroz, Luciana Domênico; Sancas, Mariana Coutinho; Brochado, Ana Carolina Batista; Spoladore, Janaína; Fonseca‐Gonçalves, Andréa; Fidalgo, Tatiana Kelly da Silva; Freitas-Fernandes, Liana Bastos; Valente, Ana Paula; Souza, Ivete Pomarico Ribeiro de; Primo, Laura Guimarães; Alves, Gutemberg Gomes

doi:10.1007/s10266-020-00541-9

Cited by 3 publications

(6 citation statements)

References 30 publications

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“…A recent study showed that Calen-ZO, inserted in 1-mm-diameter polyethylene tubes, had higher solubility than ZOE immersed in water for 35 days. Calen-ZO and ZOE had a solubility rate of 22.05% and 12.65%, respectively [ 18 ], compared with 10.54% (Calen-ZO) and 16.57% (ZOE) in 1-mm-diameter tubes in the present study. There are three possible explanations for the different results of Pintor et al [ 18 ].…”

Section: Discussionmentioning

confidence: 48%

“…Calen-ZO and ZOE had a solubility rate of 22.05% and 12.65%, respectively [ 18 ], compared with 10.54% (Calen-ZO) and 16.57% (ZOE) in 1-mm-diameter tubes in the present study. There are three possible explanations for the different results of Pintor et al [ 18 ]. (1) Although not mentioned, they probably waited for ZOE to set before immersing it in water, unlike the present study, in which the specimens were immersed immediately after handling.…”

Section: Discussionmentioning

confidence: 48%

“…Studies show that after ZOE sets, it has a solubility rate of approximately 4.5% after 30 days of immersion in distilled water [ 19 ]. (2) According to the materials studied, Pintor et al [ 18 ] used Calen in its commercial formulation, without adding zinc oxide; that is, it was a more flowable alternative. (3) The authors removed the specimens, placed them in a desiccator for 24 hr, weighed them, and then reimmersed them in water up to the next experimental time point (7, 21, 28, and 35 days).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Solubility, and Volumetric and Morphological Alterations of Bioceramic Filling Material for Primary Teeth: A New Methodological Approach

Ochoa-Rodríguez,

Coaguila-Llerena,

Fernandes

et al. 2024

International Journal of Dentistry

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Objective. To evaluate the solubility and the volumetric and morphological alterations of bioceramic filling material (Bio‐CP) for primary teeth. Materials and Methods. Bio‐CP, Calen thickened with zinc oxide (Calen‐ZO), and with zinc oxide eugenol (ZOE) were placed in 1‐ or 2‐mm‐diameter polyethylene tubes and immersed in water or phosphate‐buffered saline (PBS) for 30 days. The solubility (mass loss) was assessed using methodology modified from ISO 6876. Filling capacity, volumetric changes, and presence of voids were assessed by microcomputed tomography (micro‐CT). The surface distribution of the chemical elements and the crystalline phases was evaluated by energy scattering X‐ray scanning electron microscopy (SEM‐EDX) and X‐ray diffraction (XRD) to detect hydroxyapatite precipitate and components. The Shapiro–Wilk, Kruskal–Wallis, and Dunn’s or two‐way ANOVA and Tukey post hoc test were used (α = 0.05). Results. The solubility was ZOE > Calen‐ZO = Bio‐CP. Calen‐ZO and Bio‐CP were more soluble in water than in PBS. All the materials showed greater solubility in 2‐mm tube diameter in both PBS and distilled water, except for Bio‐CP in distilled water, which showed no difference between both tube diameters (1 and 2 mm). Only Calen‐ZO and ZOE were analyzed by micro‐CT, because Bio‐CP separated into two phases during scanning. Calen‐ZO had greater volumetric loss and presence of voids than ZOE in water, but there was no difference in PBS. The hydroxyapatite precipitate on the surface of Bio‐CP and Calen‐ZO was detected after immersion in PBS. Conclusion. Although Bio‐CP had acceptable solubility and filling capacity, its composition did not allow a proper volumetric and void assessment. From a clinical perspective, Bio‐CP has the potential to become a suitable material for root canal filling in primary teeth. Nonetheless, its composition must first be revised to achieve better chemical stability prior to its recommendation.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Solubility, and Volumetric and Morphological Alterations of Bioceramic Filling Material for Primary Teeth: A New Methodological Approach

Ochoa-Rodríguez,

Coaguila-Llerena,

Fernandes

et al. 2024

International Journal of Dentistry

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“…For example, Pintor et al recently developed a simplified model of primary root teeth that can be combined with a cell culture set-up. In this way, cells are stimulated under conditions closer to in vivo [ 71 ]. Animal models are also required to gain a better insight into the biological effects of intra-canal medications on the periodontium in order to optimize their performance.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study

Sy,

Chevalier,

Maton

et al. 2023

Antibiotics

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Endo-periodontal lesions are challenging clinical situations where both the supporting tissues and the root canal of the same tooth are infected. In the present study, chlorhexidine (CHX)-loaded calcium hydroxide (CH) pastes were used as intracanal medications (ICMs). They were prepared and tested on pathogens found in both the root canal and the periodontal pocket. Exposure to 0.5% and 1% CHX-loaded ICMs decreased the growth of Porphyromonas gingivalis and was effective in eradicating or inhibiting an Enterococcus faecalis biofilm. CH was injected into the root canal of extracted human teeth immersed in deionized water. CHX-loaded ICMs resulted in the transradicular diffusion of active components outside the tooth through the apex and the lateral dentinal tubules, as shown by the release of CHX (from 3.99 µg/mL to 51.28 µg/mL) and changes in pH (from 6.63 to 8.18) and calcium concentrations (from 2.42 ppm to 14.67 ppm) after 7 days. The 0.5% CHX-loaded ICM was non-toxic and reduced the release of IL-6 by periodontal cells stimulated by P. gingivalis lipopolysaccharides. Results indicate that the root canal may serve as a reservoir for periodontal drug delivery and that CHX-based ICMs can be an adjuvant for the control of infections and inflammation in endo-periodontal lesions.

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“…Calen paste has good to moderate cytocompatibility, with low impact on the release of pro-inflammatory cytokines and on the induction of growth factors of interest for tissue repair. And in the primary dentition, it is suggested that this paste may contribute to bone repair in clinical situations of apical periodontitis in children (PINTOR et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Filling Pastes Used in Pulp Therapy in Deciduous Teeth

Lima¹,

Lima²,

Santos³

et al. 2022

JHS

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The study on filling pastes in deciduous teeth generates a great dilemma for the pediatric dentist, as it strongly interferes with the dentition that will come. Thus, it is worth noting that, in endodontic therapy of deciduous teeth, adequate instrumentation, cleaning of the canals and an effective choice must be made associated with filling materials and their correct filling of the dental element. the most used pastes in endodontic treatment are: zinc oxide eugenol (OZE) paste, iodoformed pastes, calcium hydroxidebased paste, iodoformed pastes with calcium hydroxide and CTZ paste. The choice of material to be used must be based on the properties of each substance that it composes and the scientific evidence that supports the use of these materials. Therefore, this work aims to review the literature on filling pastes with indication for pulp treatment of deciduous teeth. This is a literature review through scientific articles and periodicals, in the "PubMed" and "Scielo" databases, published between January 2017 and December 2021. The filling pastes play an essential role in the repair of the dental element, maintaining the objective that it develops with normal biological patterns. The choice of this material is extremely important when we talk about physiological root resorption, as the paste needs to have criteria that do not interfere with the natural process. In view of the above, the success of endodontic treatment does not depend only on the material used, but it is a compilation of a detailed anamnesis, precise clinical and radiographic exams, a correct diagnosis, as well as, cautious performance of the operative technique and follow-up of the patient, keeping that deciduous tooth in the mouth until its physiological exfoliation.

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Cytocompatibility of filling pastes by primary teeth root simulating model

Cited by 3 publications

References 30 publications

Evaluation of Solubility, and Volumetric and Morphological Alterations of Bioceramic Filling Material for Primary Teeth: A New Methodological Approach

Evaluation of Solubility, and Volumetric and Morphological Alterations of Bioceramic Filling Material for Primary Teeth: A New Methodological Approach

Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study

Filling Pastes Used in Pulp Therapy in Deciduous Teeth

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