Cytochemical patterns of the peripheral blood cells in Chinese alligator (Alligator sinensis)

Chen, Xianxian; Wei, Qinqin; Wang, Jing; Peng, Fei; Li, En; Zhou, Yongkang; Zhang, Shengzhou

doi:10.1016/j.tice.2018.10.004

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…The morphological characteristics of mature erythrocytes of O. argus were similar to those of other fish (Ahmed & Sheikh, 2020;Chen et al, 2019a;Negrete et al, 2010;Vázquez & Guerrero, 2007), which were usually oval in shape with an oval or long-oval nucleus. The size of erythrocytes in O. argus was smaller than in the Chinese sturgeon (Acipenser sinensis) and sisorid catfish (Glyptosternum maculatum), larger than in the Siamese fighting fish and cichlid fish, and similar to the piebald naked carp (Table 4).…”

Section: Discussionsupporting

confidence: 68%

“…The number of thrombocytes in O. argus was lower than that of the Siamese fighting fish (Motlagh et al, 2012), shovel-nose catfish (Negrete et al, 2010), and spotted rose snapper (Rio-Zaragoza et al, 2011); higher than that of the Chinese sturgeon (Gao et al, 2007b) and piebald naked carp (Tang et al, 2015); and similar to grass carp, blunt snout bream (Megalobrama amblycephala), yellow catfish (Pelteobagrus fulvidraco) (Chen et al, 2019a;Gao et al, 2007b), and cichlid fish (Vázquez & Guerrero, 2007). The differences in the number of thrombocytes in different species of fish may be related to biotic and abiotic factors and their adaptability to the environment (Pavlidis et al, 2007;Prasad & Charles, 2010).…”

Section: The Morphology and Number Of Thrombocytes In O Argusmentioning

confidence: 91%

“…Most fish have only eosinophils without basophils (Gao et al, 2007b;Milad et al, 2016;Motlagh et al, 2012;Rio-Zaragoza et al, 2011;Vázquez & Guerrero, 2007;Zheng et al, 2016). Few fish have only basophils without eosinophils (Shigdar, Harford & Ward, 2009;Da Silva et al, 2011;Zhang et al, 2019), and some fish have neither eosinophils nor basophils (Chen et al, 2019a;Da Silva et al, 2011;Fang et al, 2014;Tavares-Dias & Moraes, 2004;Zhang et al, 2011). We did not find eosinophils and basophils in the peripheral blood of O. argus.…”

Section: The Proportion and Morphology Of Leukocytes In O Argusmentioning

confidence: 99%

“…These results were similar to those of the Murray cod (Shigdar, Harford & Ward, 2009), tilapia (Ueda et al, 2001), and piebald naked carp (Zheng et al, 2016). The thrombocytes of lower vertebrates are functionally similar to platelets in mammals (Michał, Beata & Wiesław, 2019), playing an important role in the process of hemostasis and coagulation (Chen et al, 2018;Ferdous & Scott, 2015;. Studies have investigated whether thrombocytes in some fish have phagocytotic abilities (Da Silva et al, 2011;Michał, Beata & Wiesław, 2019;Shigdar et al, 2007;Shigdar, Harford & Ward, 2009;Tavares-Dias et al, 2007;Zhang et al, 2019).…”

Section: The Cytochemical Staining Patterns Of Blood Cells In O Argusmentioning

confidence: 99%

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Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Wang

et al. 2021

PeerJ

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Background The argus snakehead (Ophiocephalus argus Cantor) is a highly nutritious, freshwater, cultured bony fish with a high economic value. The health of the fish is closely related to its blood cells, which are critical for oxygen transport, natural defense, and immunity. We investigated the morphometry, microstructure, and cytochemical characteristics of the peripheral blood cells of O. argus. Our results may provide the basic reference values needed to monitor the health of this fish for large-scale cultivation. Methods The number of blood cells in O. argus were counted on a hemocytometer and their size was measured using a micrometer under light microscope. The morphology and classification of the blood cells were studied using Wright’s staining and the cytochemical characteristics were studied using seven chemical stains including peroxidase (POX), Sudan black B (SBB), periodic acid-Schiff (PAS), acid phosphatase (ACP), alkaline phosphatase (ALP), chloroacetic acid AS-D naphthol esterase (AS-D), and α-naphthol acetate esterase (α-NAE). Results The peripheral blood cells in O. argus can be classified as erythrocytes, leukocytes, and thrombocytes; of which, females had 2.9597 million/mm3, 88,400/mm3, and 43,600/mm3, respectively, and males had 3.0105 million/mm3, 105,500/mm3, and 34,000/mm3, respectively. Leukocytes consisted of neutrophils, monocytes, large lymphocytes, and small lymphocytes. Eosinophils and basophils were not found. Monocytes were the most numerous leukocytes identified, followed by neutrophils and small lymphocytes, while large lymphocytes were the least frequently identified. Cytochemical staining showed that erythrocytes were only positive for PAS staining. Neutrophils were strongly positive for POX, SBB, and ACP, and positive for all the other cytochemical stains. Monocytes were positive for PAS and α-NAE and were weakly positive for ACP and AS-D staining. Large lymphocytes were positive for PAS and were weakly positive for ALP, AS-D, and α-NAE staining. Small lymphocytes were positive for PAS and weakly positive for AS-D and α-NAE staining. Thrombocytes were positive for PAS and were weakly positive for ACP and AS-D, but negative for the remaining cytochemical stains. The morphology of peripheral blood cells in O. argus was generally similar to that of other fish species, while the cytochemical staining patterns showed clear species specificity.

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Section: Discussionsupporting

confidence: 68%

Section: The Morphology and Number Of Thrombocytes In O Argusmentioning

confidence: 91%

Section: The Proportion and Morphology Of Leukocytes In O Argusmentioning

confidence: 99%

Section: The Cytochemical Staining Patterns Of Blood Cells In O Argusmentioning

confidence: 99%

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Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Wang

et al. 2021

PeerJ

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“…Knowledge about MPO in non-mammalian vertebrates is scarce. The enzyme has not been found in heterophils of most avian species (Genovese et al, 2013, Salakij et al, 2019, Fingerhut et al, 2020, but it has been reported in heterophils of some reptilian species (Chen et al, 2018) and in amphibian neutrophils (Bricker et al, 2012). In fish, peroxidase activity has been described in neutrophils of many teleost species (Hine and Wain, 1998).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases

Noia

Fontenla-Iglesias

Valle

et al. 2021

Developmental & Comparative Immunology

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Evaluation and Interpretation of the Peripheral Blood and Bone Marrow of Reptiles

2022

Exotic Animal Hematology and Cytology

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Cytochemical patterns of the peripheral blood cells in Chinese alligator (Alligator sinensis)

Cited by 8 publications

References 24 publications

Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Characterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases

Evaluation and Interpretation of the Peripheral Blood and Bone Marrow of Reptiles

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