2005
DOI: 10.1590/s1516-93322005000400010
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Cyclosporin A in skin samples from in vitro penetration studies may be assayed by a simple HPLC method

Abstract: (1, 10 and 20 g/mL). The coefficients of variation and deviation from the theoretical values were lower than 5%. The method described has a potential application to in vitro penetration studies of CysA using porcine skin as a biological membrane model.

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“…The recovery percentage of quercetin extracted from skin layers (SC + [E + D]) and only [E + D] was showed in Table I. By comparing the results obtained by Lopes and Bentley (2005) in terms of cyclosporine recovery from skin layers, the use of the same methodologies was more efficient in the recovery of quercetin. Thus, these high recoveries obtained for different concentrations demonstrate that the methods for quercetin extraction and quantification in different skin layers, described in the present study, were very suitable to be employed on cutaneous penetration studies.…”
Section: Resultsmentioning
confidence: 99%
“…The recovery percentage of quercetin extracted from skin layers (SC + [E + D]) and only [E + D] was showed in Table I. By comparing the results obtained by Lopes and Bentley (2005) in terms of cyclosporine recovery from skin layers, the use of the same methodologies was more efficient in the recovery of quercetin. Thus, these high recoveries obtained for different concentrations demonstrate that the methods for quercetin extraction and quantification in different skin layers, described in the present study, were very suitable to be employed on cutaneous penetration studies.…”
Section: Resultsmentioning
confidence: 99%