Cyclin A Degradation Employs Preferentially Used Lysines and a Cyclin Box Function other than Cdk1 Binding

Ramachandran, Vimal; Matzkies, Matthias; Dienemann, Axel; Sprenger, Frank

doi:10.4161/cc.6.2.3716

Cited by 13 publications

(13 citation statements)

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“…Cyclin A protein has been shown to exist in phosphorylated forms in the Drosophila embryo, with the most highly phosphorylated forms correlated with active Cyclin A/CDK1 kinase (24). Furthermore, Cyclin A recently has been demonstrated to undergo autophosphorylation (25). We observed 4 forms of Cyclin A present during oogenesis: 2 fast migrating, premeiotic forms, 4 forms in prometaphase I, the slowest migrating form in metaphase I, and all 4 forms after the completion of meiosis (Fig.…”

mentioning

confidence: 60%

“…We propose that during meiosis autophosphorylation of Cyclin A permits it to be targeted for degradation. During embryogenesis, autophosphorylation is not required for Cyclin A degradation, but in embryogenesis it is ubiquitinated by APC/C Fzy (25). There could be a distinction between the requirements of Cort and Fzy, or phosphorylation could facilitate ubiquitination but not be absolutely required.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the C terminus contains the cyclin box and is responsible for interaction between Cyclin A and CDK1. Two of the 3 principal autophosphorylation sites in Cyclin A map to the N-terminal 170 aa (T145 and S154), but a third is at S180 (25). Thus, it is striking that a single phosphoform is observed in the form of Cyclin A deleted for the N-terminal 170 aa.…”

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confidence: 99%

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Regulation of Cyclin A protein in meiosis and early embryogenesis

Vardy

Pesin

Orr‐Weaver

2009

Proc. Natl. Acad. Sci. U.S.A.

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confidence: 60%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Regulation of Cyclin A protein in meiosis and early embryogenesis

Vardy

Pesin

Orr‐Weaver

2009

Proc. Natl. Acad. Sci. U.S.A.

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“…Even the most conserved D box consensus position, the position +4 leucine, is variable. D. melanogaster cyclin A (Ramachandran et al, 2007) and H. sapiens cyclin B3 (Nguyen et al, 2002) both contain phenylalanine at this position. Several KEN box degrons also diverge from the classical consensus (Figure 2B).…”

Section: Substrate Recognition By the Apc/cmentioning

confidence: 99%

“…APC/C substrates are typically ubiquitinated at multiple lysines in disordered regions surrounding the activator-binding degron sequences (King et al, 1996; Ramachandran et al, 2007). No single ubiquitin-accepting lysine has been shown to be required for the degradation of any APC/C substrate, and multiple lysine mutations are required to stabilize a substrate.…”

Section: Substrate Ordering By the Apc/cmentioning

confidence: 99%

Building a Regulatory Network with Short Linear Sequence Motifs: Lessons from the Degrons of the Anaphase-Promoting Complex

2016

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Summary The anaphase-promoting complex or cyclosome (APC/C) is a ubiquitin ligase that polyubiquitinates specific substrates at precise times in the cell cycle, thereby triggering the events of late mitosis in a strict order. The robust substrate specificity of the APC/C prevents the potentially deleterious degradation of non-APC/C substrates, and also averts the cell cycle errors and genomic instability that could result from mistimed degradation of APC/C targets. The APC/C recognizes short linear sequence motifs, or degrons, on its substrates. The specific and timely modification and degradation of APC/C substrates is likely to be modulated by variations in degron sequence and context. We discuss the extensive affinity, specificity and selectivity determinants encoded in APC/C degrons, and we describe some of the extrinsic mechanisms that control APC/C-substrate recognition. As an archetype for protein motif-driven regulation of cell function, the APC/C-substrate interaction provides insights into the general properties of post-translational regulatory systems.

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Control of the meiotic cell division program in plants

Wijnker

Schnittger

2013

Plant Reprod

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While the question of why organisms reproduce sexually is still a matter of controversy, it is clear that the foundation of sexual reproduction is the formation of gametes with half the genomic DNA content of a somatic cell. This reduction in genomic content is accomplished through meiosis that, in contrast to mitosis, comprises two subsequent chromosome segregation steps without an intervening S phase. In addition, meiosis generates new allele combinations through the compilation of new sets of homologous chromosomes and the reciprocal exchange of chromatid segments between homologues. Progression through meiosis relies on many of the same, or at least homologous, cell cycle regulators that act in mitosis, e.g., cyclin-dependent kinases and the anaphase-promoting complex/cyclosome. However, these mitotic control factors are often differentially regulated in meiosis. In addition, several meiosis-specific cell cycle genes have been identified. We here review the increasing knowledge on meiotic cell cycle control in plants. Interestingly, plants appear to have relaxed cell cycle checkpoints in meiosis in comparison with animals and yeast and many cell cycle mutants are viable. This makes plants powerful models to study meiotic progression and allows unique modifications to their meiotic program to develop new plant-breeding strategies.

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Cyclin A Degradation Employs Preferentially Used Lysines and a Cyclin Box Function other than Cdk1 Binding

Cited by 13 publications

References 50 publications

Regulation of Cyclin A protein in meiosis and early embryogenesis

Regulation of Cyclin A protein in meiosis and early embryogenesis

Building a Regulatory Network with Short Linear Sequence Motifs: Lessons from the Degrons of the Anaphase-Promoting Complex

Control of the meiotic cell division program in plants

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