Cyclic-AMP binding to the cell surface during development of Dictyostelium discoideum

Schaap, Pauline; Spek, Wouter

doi:10.1111/j.1432-0436.1984.tb01411.x

Cited by 53 publications

(26 citation statements)

References 31 publications

(5 reference statements)

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“…However, the expression of cAMP bound as nM should be 12-fold lower after the addition of ammonium sulfate. Therefore, all data reported previously [2,3,12] remain identical, except that the apparent K a values obtained in Scatchard plots are 12-fold lower. In the present report we show that ammonium sulfate induces an approx.…”

Section: Discussionsupporting

confidence: 70%

The modulation of cell surface cAMP receptors from Dictyostelium disscoideum by ammonium sulfate

Haastert

1985

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Citation for published version (APA): Haastert, P. J. M. V. (1985). The modulation of cell surface cAMP receptors from Dictyostelium disscoideum by ammonium sulfate. Biochimica et Biophysica Acta %28BBA%29 -Molecular Cell Research, 845(2). DOI: 10.1016/0167-4889(85)90184-3 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

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Section: Discussionsupporting

confidence: 70%

The modulation of cell surface cAMP receptors from Dictyostelium disscoideum by ammonium sulfate

Haastert

1985

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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“…On the one hand, the pharmacological specificities of the various cellular responses are identical (Theibert et al 1986). In contrast, certain responses appear during late developmental stages when CARl protein levels decline dramatically (Schaap and Spek 1984;Klein et al 1987). …”

Section: Genes and Developmentmentioning

confidence: 99%

Expression of a cAMP receptor gene of Dictyostelium and evidence for a multigene family.

Saxe¹,

Johnson²,

Devreotes³

et al. 1991

Genes Dev.

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We have previously reported the cloning of cDNAs for a Dictyostelium cell-surface cAMP receptor that is a member of the family of G-protein-linked receptors. Here, we report the organization and the developmental expression of this cAMP receptor gene, designated CARl. CARl is a single copy gene that contains two intervening sequences. CARl mRNA levels are low in growing cells, rise to peak expression at 5-10 hr of development when the cAMP signaling system is maximally active, and decrease as development proceeds. At 5 hr the predominant mRNA species is -1.9 kb, by 10 hr the mRNA is heterogeneous with sizes of -1.9-2.1 kb, but during culmination only the 2.1 kb mRNA is detected. The variety of mRNA sizes results from differences in 5'-untranslated regions. Studies using developmental mutants with aberrant cAMP-signaling patterns indicate that pulsatile action of cAMP promotes maximal expression of CARl during early development. Low stringency hybridization of CARl probes to genomic DNA detects additional, related sequences, suggesting that there are several genes that encode a family of structurally similar receptors. Multiple functions previously attributed to the cAMP receptor instead may be fulfilled by distinct receptor subtypes encoded by specific genes.

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“…The prespore cells become less chemotactically sensitive to cAMP (Matsukuma and Durston, 1979;Traynor et al, 1992) by reducing their expression of certain proteins, such as cAR1 and PdsA, that are essential for generating and sensing steep chemotactic gradients (Schaap and Spek, 1984;Weening et al, 2003). This causes the prestalk cells to move selectively to the oscillating tip (Fig.…”

Section: From Unicellular Amoeba To Fruiting Bodymentioning

confidence: 99%

Evolutionary crossroads in developmental biology: Dictyostelium discoideum

Schaap

2011

Development

Self Cite

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SummaryDictyostelium discoideum belongs to a group of multicellular life forms that can also exist for long periods as single cells. This ability to shift between uni-and multicellularity makes the group ideal for studying the genetic changes that occurred at the crossroads between uni-and multicellular life. In this Primer, I discuss the mechanisms that control multicellular development in Dictyostelium discoideum and reconstruct how some of these mechanisms evolved from a stress response in the unicellular ancestor.Key words: Evolution of multicellularity, Social amoeba, Encystation, Sporulation, Dictyostelium IntroductionThe social amoebas, or Dictyostelia, are a group of organisms that become multicellular by aggregation and then proceed to build fruiting bodies that consist of stalk cells and spores (see Glossary, Box 1). This developmental cycle is a response to starvation, and involves both highly coordinated cell movement and a tightly controlled programme of cell differentiation. Social amoebas belong to the supergroup of Amoebozoa (see Glossary, Box 1), a sister group to the clade of Opisthokonts (see Glossary, Box 1), which contains the animals and fungi. All supergroups contain unicellular protist-like organisms (see Glossary, Box 1) and, in several groups, multicellular life forms have evolved independently (Minge et al., 2009).The Amoebozoa supergroup consists mainly of unicellular amoeba-like organisms that have a simple life cycle. The feeding amoeba or trophozoite turns into a dormant cyst when faced with food shortage, drought or other life-threatening circumstances (Cavalier-Smith et al., 2004). Several clades of Amoebozoa have given rise to protostelid-like organisms (see Glossary, Box 1), which form a very simple fruiting body (see Glossary, Box 1) that consists of a single spore that sits on a simple stalk made by the same cell (Shadwick et al., 2009). However, only the Dictyostelia are able to form multicellular fruiting bodies that contain up to a million cells (Fig. 1).There are ~120 known species of Dictyostelia and a molecular phylogeny has been constructed to reveal the order in which they evolved (Schaap et al., 2006). The phylogeny subdivides species into four major groups (Fig. 2), with the model social amoeba Dictyostelium discoideum belonging to the most recently diverged group 4. Many species in groups 1-3 can still encyst as single cells when conditions are unfavourable for aggregation. However, group 4 species have lost the ability to encyst. Strikingly, all group 4 species tested secrete cyclic adenosine monophosphate (cAMP) to act as a chemoattractant for aggregation. This is most unusual, because almost all other organisms only use cAMP inside the cell to transduce the effect of other secreted stimuli, such as hormones, mating factors and neurotransmitters. None of the species in groups 1-3 uses cAMP for aggregation; some use glorin, a modified dipeptide of glutamate and ornithine, whereas others use folic acid, pterin or other as yet unidentified chemoattractants (Schaap e...

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Cyclic-AMP binding to the cell surface during development of Dictyostelium discoideum

Cited by 53 publications

References 31 publications

The modulation of cell surface cAMP receptors from Dictyostelium disscoideum by ammonium sulfate

The modulation of cell surface cAMP receptors from Dictyostelium disscoideum by ammonium sulfate

Expression of a cAMP receptor gene of Dictyostelium and evidence for a multigene family.

Evolutionary crossroads in developmental biology: Dictyostelium discoideum

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