Cyanotoxin Screening in BACA Culture Collection: Identification of New Cylindrospermopsin Producing Cyanobacteria

Cordeiro, Rita; Azevedo, Joana; Luz, Rúben; Vasconcelos, Vı́tor; Gonçalves, Vítor; Fonseca, Amélia

doi:10.3390/toxins13040258

Cited by 9 publications

(4 citation statements)

References 73 publications

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“…In the case of C. raciborskii , a species-specific primer was developed [ 26 ], and molecular biological monitoring using real-time PCR analysis was conducted [ 27 , 28 ]. However, most studies on INC primarily comprise morphological microscopic analyses [ 16 , 29 , 30 ] or only focus on toxin-related genes [ 28 , 31 , 32 ]. Recently, genus-specific primers that can detect five genera of INC ( Cuspidothrix , Cylindrospermopsis , Sphaerospermopsis , Raphidiopsis , and Chrysosperum ) were developed [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Appearance and Toxin Production Potential of Invasive Nostocalean Cyanobacteria Using Quantitative Gene Analysis in Nakdong River, Korea

Park

Kim

2022

Toxins

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Invasive nostocalean cyanobacteria (INC) were first reported in tropical regions and are now globally spreading rapidly due to climate change, appearing in temperate regions. INC require continuous monitoring for water resource management because of their high toxin production potential. However, it is difficult to analyze INC under a microscope because of their morphological similarity to nostocalean cyanobacteria such as the genus Aphanizomenon. This study calculates the gene copy number per cell for each target gene through quantitative gene analysis on the basis of genus-specific primers of genera Cylindrospermopsis, Sphaerospermopsis, and Cuspidothrix, and the toxin primers of anatoxin-a, saxitoxin, and cylindrospermopsin. In addition, quantitative gene analysis was performed at eight sites in the Nakdong River to assess the appearance of INC and their toxin production potential. Genera Cylindrospermopsis and Sphaerospermopsis did not exceed 100 cells mL−1 at the maximum, with a low likelihood of related toxin occurrence. The genus Cuspidothrix showed the highest cell density (1759 cells mL−1) among the INC. Nakdong River has potential for the occurrence of anatoxin-a through biosynthesis by genus Cuspidothrix because the appearance of this genus coincided with that of the anatoxin-a synthesis gene (anaF) and the detection of the toxin by ELISA.

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Section: Introductionmentioning

confidence: 99%

Assessment of the Appearance and Toxin Production Potential of Invasive Nostocalean Cyanobacteria Using Quantitative Gene Analysis in Nakdong River, Korea

Park

Kim

2022

Toxins

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“… Strain MCs CYN STX ANT BMAA NOD Reference Stanieria cyanosphaera sp. * 55 Chroococcidiopsis sp. * + 56 Gomphosphaeria sp.…”

Section: Resultsmentioning

confidence: 99%

Using FlowCam and molecular techniques to assess the diversity of Cyanobacteria species in water used for food production

Mutoti

Jideani

Gumbo

2022

Sci Rep

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Globally, the occurrence of cyanobacteria in water currently remains an important subject as they produce cyanotoxins that pose threat to human health. Studies on the contamination of maize meals during mill grinding processes using cyanobacteria-contaminated water have not been conducted. The present study aimed to assess the diversity of cyanobacteria in the samples (process water, uncooked maize meal, and cooked maize meal (porridge)). Polymerized Chain Reaction (PCR) and Advanced digital flow cytometry (FlowCAM) were used to detect and identify cyanobacterial species available in these samples. 16S Primers (forward and reverse) tailed with Universal Sequences were used for amplification and sequencing of full-length 16S rRNA genes from cyanobacteria found in all samples. Cyanobacterial species from order Nostocales, Pseudanabaenales, Oscillatoriales Chroococcales, Synechococcales, and unclassified cyanobacterial order, some of which have the potential to produce cyanotoxins were amplified and identified in process water, raw maize meal and porridge samples using PCR. Images of the genus Microcystis, Phormidium, and Leptolyngbya were captured in process water samples using FlowCAM. These findings show the presence of cyanobacteria species in process water used for maize meal and the absence in cooked maize meal. The presence of cyanobacteria in process water is likely another route of human exposure to cyanotoxins.

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“…Strains with more than one band amplified had the PCR product cloned using the NZY‐A PCR cloning kit (Nzytech, Portugal), following the manufacturer's protocol. Sequencing of the 16S rRNA gene and 16S–23S ITS rRNA region was conducted using the 27F (Neilan et al., 1997), 781F (Cordeiro et al., 2021), 781R (Nübel et al., 1997), CSIF (Janse et al., 2004), and 23S30R primers (Lepère et al., 2000).…”

Section: Methodsmentioning

confidence: 99%

Description of four new filamentous cyanobacterial taxa from freshwater habitats in the Azores Archipelago

Luz,

Cordeiro,

Kaštovský

et al. 2023

Journal of Phycology

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Simple filamentous cyanobacteria comprise a diverse and polyphyletic group of species, primarily in the orders Leptolyngbyales and Oscillatoriales, that need more sampling to improve their taxonomy. Oceanic islands, such as the Azores archipelago, present unique habitats and biogeographic conditions that harbor an unknown range of diversity of microorganisms. Filamentous cyanobacteria isolated from aquatic habitats in the Azores and maintained in the BACA culture collection were described using morphology, both light and transmission electron microscopy, ecology, and genetic data of the 16S rRNA gene sequences and 16S–23S Internal Transcribed Spacer (ITS) rRNA region secondary structure. Our analyses revealed two new monophyletic genera: Tumidithrix elongata gen. sp. nov. (Pseudanabaenaceae) and Radiculonema aquaticum gen. sp. nov. (Leptolyngbyaceae). In addition, two new species Leptodesmis lacustris sp. nov. (Leptolyngbyaceae) and Pycnacronema lacustrum sp. nov. (Wilmottiaceae) are reported as the first aquatic species for these genera. The description of these new taxa and the genetic study of an isolate of Leptodesmis alaskaensis from the Azores followed the polyphasic approach, identifying diacritical features. Our results reinforce the need for taxonomic studies on cyanobacteria from less‐studied habits and geographic regions, which have a potential for new taxa description.

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Cyanotoxin Screening in BACA Culture Collection: Identification of New Cylindrospermopsin Producing Cyanobacteria

Cited by 9 publications

References 73 publications

Assessment of the Appearance and Toxin Production Potential of Invasive Nostocalean Cyanobacteria Using Quantitative Gene Analysis in Nakdong River, Korea

Assessment of the Appearance and Toxin Production Potential of Invasive Nostocalean Cyanobacteria Using Quantitative Gene Analysis in Nakdong River, Korea

Using FlowCam and molecular techniques to assess the diversity of Cyanobacteria species in water used for food production

Description of four new filamentous cyanobacterial taxa from freshwater habitats in the Azores Archipelago

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