CvL, a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotes

Abstract

CvL, a lectin from the marine sponge Cliona varians was purified by acetone fractionation followed by Sepharose CL 4B affinity chromatography. CvL agglutinated papainized treated human erythrocytes with preference for type A erythrocytes. The lectin was strongly inhibited by monosaccharide d-galactose and disaccharide sucrose. CvL is a tetrameric glycoprotein of 28 kDa subunits linked by disulphide bridges with a molecular mass of 106 kDa by SDS-PAGE and 114 kDa by Sephacryl S300 gel filtration. The lectin was Ca2+ dependent, stable up to 60 degrees C for 60 min, with optimum pH of 7.5. CvL displays a cytotoxic effect on gram positive bacteria, such as Bacillus subtilis and Staphylococcus aureus. However, CvL did not affect gram negative bacteria, such as Escherichia coli and Pseudomonas aeruginosa. Leishmania chagasi promastigotes were agglutinated by CvL up to 2(8) titer. These findings are indicative of the physiological defense roles of CvL and its possible use in the antibiosis of bacteria and protozoa pathogenic.

Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

“…To date, the tropical sponge Cliona varians possesses one isolated lectin, CvL-1. CvL-1 is a galactose-binding lectin with cytotoxic effect on pathogenic bacteria, such as Bacillus subtilis and Staphylococcus aureus (Moura et al 2006). Furthermore, the sea cucumber Holothurea grisea possesses two isolated lectins: HGA, an anti-inflammatory lectin of 228 kDa, and HGA-2, a C-type lectin of 34 kDa (Moura et al 2012, Melo et al 2014.…”

Purification, characterization and induction of a C-type lectin in the freshwater planarian Dugesia japonica

“…It is similar to lectins (C-type) in the body surface mucus of D. japonica [18]. Also, the majority of invertebrate lectins are C-type lectins [14,17,[40][41][42][43][44], which require calcium for their agglutinating activity. However, it differs from D-galactoside-specific lectins in amphioxus [11], lectins in the shrimp Litopenaeus schmitti [45] and lectins in the sea cucumber [12].…”

“…Furthermore, lectins can be induced by bacteria or by injury in a number of invertebrates [9][10][11][12][13]. Given such important functions, numerous investigations have been carried out on the characterization and function of lectins in cephalochordate [11] and in invertebrates [14][15][16][17].…”

Leishmanicidal activity of Echinaster (Othilia) echinophorus crude extract

“…These new drugs have been approved for the treatment of cancer, neurological diseases, infectious diseases, cardiovascular and metabolic diseases 5 . In this sense, previous compounds isolated from marine organism have been reported with antileishmanial activity; these include a glycoprotein isolated from Pachymatisma johnstoni 24 , a lectin from Cliona varians 27 and three sulfated meroterpenoids from Callyspongia sp. 16 .…”