“…These three factors are co-dependent for localization to cluster heterochromatin, drive non-canonical transcription of piRNA clusters from both genomic strands, suppress cluster transcript splicing and polyadenylation, and promote piRNA biogenesis (Andersen et al, 2017; Chen et al, 2016; Klattenhoff et al, 2009; Mohn et al, 2014; Pane et al, 2011; Parhad et al, 2017; Zhang et al, 2014). Like the founding member of the HP1 family, HP1a, Rhi binds to trimethylated lysine 9 of histone 3 (H3K9me3) through its C-terminal chromo domain (Le Thomas et al, 2014; Mohn et al, 2014; Yu et al, 2015).…”