Cuticular proteins and seasonal photoperiodism in aphids

Gallot, Aurore; Rispe, Claude; Leterme, Nathalie; Gauthier, Jean-Pierre; Jaubert-Possamai, Stéphanie; Tagu, Denis

doi:10.1016/j.ibmb.2009.12.001

Cited by 79 publications

(67 citation statements)

References 35 publications

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“…This represents the largest number of CPR genes in any insect genome annotated to date and is 36% greater than the number of CPR genes in the silkworm B. mori : 56 RR-1, 93 RR-2, and 4 RR-3; 149 were originally annotated by Futahashi et al (2008) including the misclassified CPH5, three more were added by Liang et al (2010), and one additional gene, LOC101743422, was discovered in our analysis. Similar to observations for other insects (Cornman et al ., 2008; Dittmer et al ., 2012; Gallot et al ., 2010; Liang et al ., 2010), many of the genes occurred in clusters: 55 of the RR-1 genes were found in clusters of 11-17 genes on four scaffolds (00081, 00136, 00224, 00529), while 86 of the RR-2 genes were found in clusters of 8-26 genes on six scaffolds (00006, 00227, 00685, 00717, 01004, 01013) (Table 1). …”

Section: Resultsmentioning

confidence: 99%

“…Combining proteomics with genomics has been used to identify cuticular proteins for several insect species (Bae et al ., 2011; Carrasco et al ., 2011; Dittmer et al ., 2012; Fu et al ., 2011; He et al ., 2007). Similarly, genomics and transcriptomics have contributed valuable information on CP gene expression, discerning variations in the timing (pre- or post-molt), developmental stage (larval, pupal, adult), and relative expression levels of these genes (Cornman and Willis, 2009; Dittmer et al ., 2012; Futahashi et al ., 2008; Gallot et al ., 2010; Liang et al ., 2010; Okamoto et al ., 2008; Suetsugu et al ., 2013; Togawa et al ., 2007, 2008). …”

Section: Introductionmentioning

confidence: 99%

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Annotation and expression analysis of cuticular proteins from the tobacco hornworm, Manduca sexta

Dittmer

Tetreau

Cao

et al. 2015

Insect Biochemistry and Molecular Biology

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The insect cuticle is a unique material that covers the exterior of the animal as well as lining the foregut, hindgut, and tracheae. It offers protection from predators and desiccation, defines body shape, and serves as an attachment site for internal organs and muscle. It has demonstrated remarkable variations in hardness, flexibility and elasticity, all the while being light weight, which allows for ease of movement and flight. It is composed primarily of chitin, proteins, catecholamines, and lipids. Proteomic analyses of cuticle from different life stages and species of insects has allowed for a more detailed examination of the protein content and how it relates to cuticle mechanical properties. It is now recognized that several groups of cuticular proteins exist and that they can be classified according to conserved amino acid sequence motifs. We have annotated the genome of the tobacco hornworm, Manduca sexta, for genes that encode putative cuticular proteins that belong to seven different groups: proteins with a Rebers and Riddiford motif (CPR), proteins analogous to peritrophins (CPAP), proteins with a tweedle motif (CPT), proteins with a 44 amino acid motif (CPF), proteins that are CPF-like (CPFL), proteins with an 18 amino acid motif (18 aa), and proteins with two to three copies of a C-X5-C motif (CPCFC). In total we annotated 248 genes, of which 207 belong to the CPR family, the most for any insect genome annotated to date. Additionally, we discovered new members of the CPAP family and determined that orthologous genes are present in other insects. We established orthology between the M. sexta and Bombyx mori genes and identified duplication events that occurred after separation of the two species. Finally, we utilized 52 RNAseq libraries to ascertain gene expression profiles that revealed commonalities and differences between different tissues and developmental stages.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Annotation and expression analysis of cuticular proteins from the tobacco hornworm, Manduca sexta

Dittmer

Tetreau

Cao

et al. 2015

Insect Biochemistry and Molecular Biology

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“…The use of controlled conditions in laboratory has identified the photoperiod as the main and sufficient signal that triggers this switch (Lees, 1959(Lees, , 1960. The bases of seasonal photoperiodism in aphids are partially known owing to physiological and molecular analyses performed in controlled laboratory conditions (see Le Trionnaire et al, 2008;Gallot et al, 2010 for a recent review). Several hypotheses on the role of neurosecretory materials or hormone regulators in the sensing and signalling of photoperiodic cues have been proposed (Le Trionnaire et al, 2007, 2009Cortes et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiling of the reproductive mode switch in the pea aphid in response to natural autumnal photoperiod

Trionnaire

Jaubert-Possamai

Bonhomme

et al. 2012

Journal of Insect Physiology

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“…Functional genomics has identified several genes that are regulated during photoperiod shortening, many of them being involved in neuro-endocrine signaling (e.g., insulin, dopamine; Le Trionnaire et al 2007Trionnaire et al , 2009Gallot et al 2010). The genome annotation of A. pisum has allowed the identification of 42 neuropeptides and neurohormones (Huybrechts et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Revisiting the anatomy of the central nervous system of a hemimetabolous model insect species: the pea aphid Acyrthosiphon pisum

Kollmann

Minoli

Bonhomme³

et al. 2010

Cell Tissue Res

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Aphids show a marked phenotypic plasticity, producing asexual or sexual and winged or wingless morphs depending on environmental conditions and season. We describe here the general structure of the brain of various morphs of the pea aphid Acyrthosiphon pisum. This is the first detailed anatomical study of the central nervous system of an aphid by immunocytochemistry (synapsin, serotonin, and several neuropeptides), ethyl-gallate staining, confocal laser scanning microscopy, and three-dimensional reconstructions. The study has revealed well-developed optic lobes composed of lamina, medulla, and lobula complex. Ocelli are only present in males and winged parthenogenetic females. The central complex is well-defined, with a central body divided into two parts, a protocerebral bridge, and affiliated lateral accessory lobes. The mushroom bodies are ill-defined, lacking calyces, and only being visualized by using an antiserum against the neuropeptide orcokinin. The antennal lobes contain poorly delineated glomeruli but can be clearly visualized by performing antennal backfills. On the basis of our detailed description of the brain of winged and wingless parthenogenetic A. pisum females, an anatomical map is now available that should improve our knowledge of the way that these structures are involved in the regulation of phenotypic plasticity.

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Cuticular proteins and seasonal photoperiodism in aphids

Cited by 79 publications

References 35 publications

Annotation and expression analysis of cuticular proteins from the tobacco hornworm, Manduca sexta

Annotation and expression analysis of cuticular proteins from the tobacco hornworm, Manduca sexta

Transcriptomic profiling of the reproductive mode switch in the pea aphid in response to natural autumnal photoperiod

Revisiting the anatomy of the central nervous system of a hemimetabolous model insect species: the pea aphid Acyrthosiphon pisum

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