2002
DOI: 10.1001/archderm.138.2.271
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Cutaneous Infection Showing Sporotrichoid Spread Caused by Pseudallescheria boydii (Scedosporium apiospermum): Successful Detection of Fungal DNA in Formalin-Fixed, Paraffin-Embedded Sections by Seminested PCR

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Cited by 24 publications
(14 citation statements)
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“…In the immunocompromised host (people receiving chronic steroid therapy for chronic obstructive pulmonary disease or pulmonary fibrosis or receiving immunosuppressive therapy for rheumatoid arthritis), soft tissue infections due to S. prolificans are on the rise (35,124,312,423). A syndrome of nodular lymphangitis or lymphocutaneous syndrome due Scedosporium apiospermum has been reported (61,180,221,229,445).…”
Section: Ii) Extrapulmonary Infections (A) Cutaneous and Subcutaneoumentioning
confidence: 99%
“…In the immunocompromised host (people receiving chronic steroid therapy for chronic obstructive pulmonary disease or pulmonary fibrosis or receiving immunosuppressive therapy for rheumatoid arthritis), soft tissue infections due to S. prolificans are on the rise (35,124,312,423). A syndrome of nodular lymphangitis or lymphocutaneous syndrome due Scedosporium apiospermum has been reported (61,180,221,229,445).…”
Section: Ii) Extrapulmonary Infections (A) Cutaneous and Subcutaneoumentioning
confidence: 99%
“…PCR-based assays from infected tissue are useful for rapid diagnosis of S. apiospermumi infections, even before fungal cultures become positive [55]. Wedde et al .…”
Section: Molecular Identificationmentioning
confidence: 99%
“…Cutaneous infections are infrequent and mostly are associated with immunodepression [55,191], e.g., in solid transplant recipients [192]. An interdigital case was reported by Karaarslan et al .…”
Section: Characteristics Of Diseasementioning
confidence: 99%
“…Wedde et al (22) developed a conventional PCR assay that consisted in the amplification of an rDNA region and detection with hybridization probes for the clinically relevant Scedosporium species. Other authors designed PCR-based assays for the detection of Scedosporium DNA from infected tissues (11,14). Recently, a microarray assay for the detection of fungal DNA from 14 pathogen species, including S. prolificans, has been designed (20).…”
Section: Discussionmentioning
confidence: 99%
“…An assay based on the detection of a peptidorhamnomannan antigen for S. apiospermum is available (15); however, cross-reactivity with other fungal species has been reported (13). Finally, conventional PCR assays have been developed to detect Scedosporium DNA (1,11,22), but to date no methods based on quantitative PCR methodologies to detect these species have been described.…”
mentioning
confidence: 99%