Cutaneous applied nano-ZnO reduce the ability of hair follicle stem cells to differentiate

Ge, Wei; Zhao, Yong; Lai, Fang-Nong; Liu, Jing-Cai; Sun, Ying; Wang, Junjie; Cheng, Shun‐Feng; Zhang, Xifeng; Sun, Li-Lan; Li, Lan; Dyce, Paul W.; Shen, Wei

doi:10.1080/17435390.2017.1310947

Cited by 38 publications

(41 citation statements)

References 46 publications

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“…Mice were maintained under a light: dark cycle of 12:12 h and at a temperature of 23 o C and humidity of 50-70%; they had free access to food (chow diet) and water. 36 In our preliminary study (based on early report: Wan et al 22 ), AOS 10 mg/kg body weight dosing for two weeks was the best treatment condition for improving busulfan disrupted small intestine (1, 5, 10, 20 mg/kg body weight of AOS dosing for 1, 2, 3, and 4 weeks). Three-week-old ICR male mice were given a single injection of 40 mg/kg body weight (BW) of busulfan.…”

Section: Materials and Methods [Detailed Methods In Supplemental Infomentioning

confidence: 78%

“…39 Ultrastructural analysis of small intestine tissues by transmission electron microscopy (TEM) The procedure for TEM analysis has been reported in our early article. 36 Fifty nanometer sections were cut on a Leica Ultracut E equipped with a diamond knife (Diatome, Hatfield, PA). The sections were stained with uranyl acetate and viewed on a JEM-2010F TEM (JEOL Ltd., Japan).…”

Section: Materials and Methods [Detailed Methods In Supplemental Infomentioning

confidence: 99%

“…The procedure for IHF staining was reported in our recent publications. 36,44 Table S1 lists the primary antibodies.…”

Section: Immunofluorescent Staining (Ihf)mentioning

confidence: 99%

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Single-cell RNA sequencing analysis reveals alginate oligosaccharides preventing chemotherapy-induced mucositis

et al. 2020

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Worldwide the incidence of cancer has been continuing increasing. Mucositis of the gastrointestinal tract is a common side effect in patients under chemotherapy. Anticancer drug busulfan, used for treating chronic myeloid leukemia especially in pediatric patients, causes mucositis of the gastrointestinal tract. Alginate oligosaccharides (AOS) are natural products with attractive pharmaceutical potentials. We aimed to investigate, at the single-cell level, AOS preventing small intestine mucositis induced by busulfan. We found that busulfan disturbed the endoplasmic reticulum and mitochondria of cells in the small intestine, damaged cell membranes especially cell junctions, and disrupted microvilli; all of which were rescued by AOS. Single-cell RNA sequencing analysis and functional enrichment analysis showed that AOS could recover small intestinal function. Deep analysis found that AOS improved the expression of transcriptional factors which explained AOS regulating gene expression to improve small intestine function. Further investigation in IPEC-J2 cells found that AOS acts its function through mannose receptor signaling pathway. Moreover, the improved blood metabolome confirmed small intestinal function was recovered by AOS. As a natural product with many advantages, AOS could be developed to assist in the recovery of intestinal functions in patients undergoing anticancer chemotherapy or other treatments.

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Section: Materials and Methods [Detailed Methods In Supplemental Infomentioning

confidence: 78%

Section: Materials and Methods [Detailed Methods In Supplemental Infomentioning

confidence: 99%

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Single-cell RNA sequencing analysis reveals alginate oligosaccharides preventing chemotherapy-induced mucositis

et al. 2020

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“…The immunofluorescence or enzyme horseradish peroxidase (HRP)-based immunohistochemistry analysis procedure was performed as we previously described (Ge et al, 2017, Liu et al, 2017). For immunofluorescence analysis, the paraffin-embedded skin tissues were firstly deparaffinized in xylene and further rehydrated in ethanol solutions.…”

Section: Methodsmentioning

confidence: 99%

A single-cell transcriptome atlas during Cashmere goat hair follicle morphogenesis

Zhang

et al. 2020

Preprint

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Cashmere, also known as soft gold, is produced from the secondary hair follicles in Cashmere goats and it’s therefore of significance to investigate the molecular profiles during Cashmere goat hair follicle development. However, our current understanding of the machinery underlying Cashmere goat hair follicle remains largely unexplored and researches regarding hair follicle development mainly used the mouse as a research model. To provides comprehensively understanding on the cellular heterogeneity and cell lineage cell fate decisions, we performed single-cell RNA sequencing on 19,705 single cells from induction (embryonic day 60), organogenesis (embryonic day 90) and cytodifferentiation (embryonic day 120) stages of fetus Cashmere goat dorsal skin. Unsupervised clustering analysis identified 16 cell clusters and their corresponding cell types were also unprecedentedly characterized. Based on the lineage inference, we revealed detailed molecular landscape along the dermal and epidermal cell lineage developmental pathways. Notably, by cross-species comparasion of single cell data with murine model, we revelaed conserved programs during dermal condensate fate commitment and the heterochrony development of hair follicle development between mouse and Cashmere goat were also discussed here. Our work here delineate unparalleled molecular profiles of different cell populations during Cashmere goat hair follicle morphogenesis and provide a valuable resource for identifying biomarkers during Cashmere goat hair follicle development.

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“…Then they were send to sequence using HiSeq2500 sequencer at Novogene Bioinformatics Technology Co., Ltd (Beijing, China). Bioinformatic analyses of the data were then performed according the workflow described previously (23,24).…”

Section: Rna Extraction Reverse Transcription and Rna-seqmentioning

confidence: 99%

Di (2-ethylhexyl) Phthalate Exposure Impairs the microRNAs Expression Profile During Primordial Follicle Assembly

Zhang

Liu

et al. 2019

Front. Endocrinol.

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This research was performed to estimate the potential effects of Di (2-ethylhexyl) phthalate (DEHP) on changes of ovarian miRNA expression profile during mouse primordial follicle assembly using miRNAs-seq analysis. The ovaries of newborn mice were collected and in vitro cultured with different concentration of DEHP for 72 h. Then they were prepared for miRNAs-seq analysis. The results indicated that DEHP exposure altered ovarian miRNA expression profile of newborn mice. Eighteen differentially expressed miRNAs were screened after 100 µM DEHP exposure. The target mRNAs of differentially expressed miRNAs were predicted and further analyzed through gene ontology (GO) enrichment analysis and pathway enrichment analysis. Our results showed that the differentially expressed miRNAs from DEHP exposure can regulate ovarian development by targeting mRNAs involved in MAPK, mTOR, FoxO signaling pathways. Three miRNAs of miR-32-5p, miR-19a-3p, and miR-141-3p were randomly selected from the differentially expressed miRNAs to quantify their expression level by miRNA qRT-PCR. The results of qRT-PCR and miRNA-seq were consistent. Considering one of its target gene PTEN of miR-19a-3p and the decreased level of pAKT and increased Bax/Bcl-2 under DEHP exposure, we speculated that the altered expression of miR-19a-3p by DEHP exposure affected mouse primordial follicle assembly via PI3K/AKT1/mTOR signaling pathway. Epigenetic changes are one of the most important targets of toxicant exposure. The effects of DEHP exposure on microRNA (one of the epigenetic regulators) expression profile were uncovered to enrich the research on relationship of epigenetics and toxicant exposure.

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Cutaneous applied nano-ZnO reduce the ability of hair follicle stem cells to differentiate

Cited by 38 publications

References 46 publications

Single-cell RNA sequencing analysis reveals alginate oligosaccharides preventing chemotherapy-induced mucositis

Single-cell RNA sequencing analysis reveals alginate oligosaccharides preventing chemotherapy-induced mucositis

A single-cell transcriptome atlas during Cashmere goat hair follicle morphogenesis

Di (2-ethylhexyl) Phthalate Exposure Impairs the microRNAs Expression Profile During Primordial Follicle Assembly

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