Current research progress on laccase-like nanomaterials

Tan

et al. 2023

Anal. Chem.

Nanozymes can imitate the catalytic properties of natural enzymes while overcoming the limitations of natural enzymes such as high cost, poor robustness, and difficulty in recycling. However, rational design and facile preparation of nanozymes are still in demand. Inspired by the chemical structure of laccase, we report an amorphous metal–organic coordination nanocomposite named CuNAD, which is composed of copper ions and nicotinamide adenine dinucleotide (NAD+) via a simple coordinating coassembly process. As a single-site nanozyme, CuNAD exhibits excellent robustness under extreme conditions, significantly stronger catalytic activity for phenolic compounds, and 4.02-fold higher sensitivity for epinephrine detection than laccase. Furthermore, by breaking through the functional constraints of laccase, CuNAD is also able to activate H2O2 at neutral pH, benefiting a one-step chromogenic detection platform for cholesterol. This facile approach demonstrates the potential to develop single-site nanozymes by biomimicking natural enzymes and may boost more insights into the structure–function relationship of nanozymes.

Section: Catalytic Performance Of Cunad For Polyphenolmentioning

confidence: 99%

Bioinspired Coassembly of Copper Ions and Nicotinamide Adenine Dinucleotides for Single-Site Nanozyme with Dual Catalytic Functions

Tan

et al. 2023

Anal. Chem.

“…Since scientists discovered the first kind of inorganic nanometer material Fe 3 O 4 with catalytic activity, thorough research began to be carried out for other nanometer materials. − Some of them show similar catalytic activities to natural enzymes, and the catalytic reactions conform to the basic kinetic principles and mechanisms of enzymes, which are called nanozymes. Compared to natural enzymes, they exhibit similar catalytic activity, simple preparation, and stable properties, − thus show promising applications in many fields.…”

Section: Introductionmentioning

confidence: 99%

Choline Oxidase-Integrated Copper Metal–Organic Frameworks as Cascade Nanozymes for One-Step Colorimetric Choline Detection

Huang

Song

et al. 2022

J. Agric. Food Chem.

Self Cite

Choline is an important factor for regulating human health and is widely present in various foods. In this work, a sensor strategy based on a choline oxidase-integrated copper(II) metal−organic framework with peroxidase-like activity is constructed for one-step cascade detection of choline. The one-step cascade strategy can avoid intermediate product transferring in general multi-step reactions, and the multi-enzyme activities can be well exerted under one condition, thus exhibiting excellent catalytic activity and enhanced stability. In the integrated system, choline is catalyzed by ChO x to produce betaine and H 2 O 2 , which eventually got converted to hydroxyl radicals by the peroxidase nanozyme, oxidized the chromogenic substrate ABTS, and produced an observable absorption peak at 420 nm. A new choline detection method was thus established and showed a satisfactory linear relationship at 6−300 μM, which has been used for the choline analysis in milk.

“…However, the inherent lack of fine structure in nanozymes leads to low specificity for targeted substrates, , which fails to replace natural enzymes completely in practical applications, especially in the analysis and detection of specific substances. For example, natural polyphenol oxidase is often used to quantify phenols, − which play an important role in human life. , While most of the explored oxidase nanozymes lack enzyme-like selectivity, − they generally catalyze the substrate oxidation by activating O 2 to produce reactive oxygen species. Thus, they can not only oxidize phenolic compounds but also catalyze a series of reducing substrates containing 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt (ABTS), 3,3′,5,5′-tetramethylbenzidine (TMB), o -phenylenediamine (OPD), and glutathione.…”

Section: Introductionmentioning

confidence: 99%

Solid Migration to Assemble a Flower-like Nanozyme with Highly Dense Single Copper Sites for Specific Phenol Oxidation

Chen

ACS Appl. Mater. Interfaces

Tian

et al. 2022

Nanozymes with high catalytic stability and sustainability have emerged as powerful competitors to natural enzymes for diverse biocatalytic applications. However, constructing a nanozyme with high specificity is one of their biggest challenges. Herein, we develop a facile solid migration strategy to access a flower-like single copper site nanozyme (Cu SSN) via direct transformation of copper foam activated by 2-methylimidazole. With highly clustered CuN3 sites whose local structure is similar to that of natural polyphenol oxidase, the Cu SSN exhibits excellent activity and specificity to oxidize phenols without peroxidase-like activity. Furthermore, the Cu SSN shows high sensitivity in the colorimetric detection of epinephrine with a low detection limit of 0.10 μg mL–1, exceeding that of most previously reported enzyme-mimicking catalysts. This work not only provides a simple method for the large-scale preparation of high-performance nanozymes but also offers an inspiration for the design of highly specific nanozymes by mimicking the synergy among sites in natural enzymes.