Current perspectives in protein array technology

Kricka, Larry J.; Master, Stephen R.; Joos, Thomas; Fortina, Paolo

doi:10.1258/000456306778904731

Cited by 62 publications

(48 citation statements)

References 81 publications

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“…cytokines and adhesion molecules) in a single patient sample and provides valuable information relating to each tested ana-lyte and possible associations between analytes in each sample 15,16 . The core technology is the biochip, a solidstate device containing an array of discrete test regions of immobilised antibodies specific to different cytokines and adhesion molecules.…”

Section: Multi-analytical Evaluation Using Biochip Array Technologymentioning

confidence: 99%

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

Horáček¹,

Kupsa²,

Vašatová³

et al. 2015

BIOMED PAP

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Aims.To compare serum levels of 17 cytokines and 5 adhesion molecules in patients with newly diagnosed acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) using biochip array technology. Methods. A total of 15 AML and 15 ALL patients were studied. Serum samples were taken prior to anticancer therapy and were analyzed by biochip based immunoassays on the Evidence Investigator analyzer. This approach allows simultaneous detection of multiple analytes from a single sample. T-tests were used for statistical analysis. Results. Comparing cytokine and adhesion molecules levels in newly diagnosed AML and ALL patients, we found significant increase in AML in serum IL-4 (P < 0.0001), IL-2 (P < 0.01), IL-3 (P < 0.05), and significant decrease (P < 0.05) in serum VEGF and VCAM-1. Discussion. Our results indicate that serum profile of cytokines and adhesion molecules differs in newly diagnosed AML and ALL patients. Further studies are needed to establish if these alterations could be used as a clinically relevant biomarker for acute leukemias.

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Section: Multi-analytical Evaluation Using Biochip Array Technologymentioning

confidence: 99%

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

Horáček¹,

Kupsa²,

Vašatová³

et al. 2015

BIOMED PAP

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“…In these assays, the need to separate unbound probe molecules from bound ones is avoided and this provides significant advantages such as convenience, speed, and accuracy of DNA analysis. [2][3][4][5] Most of the current specific detection systems rely on the use of labeled oligonucleotide-based probe molecules that bind to their DNA target in a sequence-specific manner. Most commonly, bound probes are distinguished from unbound probes by hybridization-induced spatial convergence or separation of two markers that interact through fluorescence resonance energy transfer (FRET).…”

Section: Introductionmentioning

confidence: 99%

Forced Intercalation Probes (FIT Probes): Thiazole Orange as a Fluorescent Base in Peptide Nucleic Acids for Homogeneous Single‐Nucleotide‐Polymorphism Detection

Köhler¹,

Jarikote²,

Seitz³

2005

ChemBioChem

221

172

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Fluorescent base analogues in DNA are versatile probes of nucleic acid-nucleic acid and nucleic acid-protein interactions. New peptide nucleic acid (PNA) based probes are described in which the intercalator dye thiazole orange (TO) serves as a base surrogate. The investigation of six TO derivatives revealed that the linker length and the conjugation site decided whether a base surrogate conveys sequence-selective DNA binding and whether fluorescence is increased or decreased upon single-mismatched hybridization. One TO derivative conferred universal PNA-DNA base pairing while maintaining duplex stability and hybridization selectivity. TO fluorescence increased up to 26-fold upon hybridization. In contrast to most other probes, in which fluorescence is invariant once hybridization had occurred, the emission of TO-containing PNA probes is attenuated when forced to intercalate next to a mismatched base pair. The specificity of DNA detection is therefore not limited by the selectivity of probe-target binding and a DNA target can be distinguished from its single-base mutant under nonstringent hybridization conditions. This property should be of advantage for real-time quantitative PCR and nucleic acid detection within living cells.

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“…[6] These attractive characteristics enabled DNA to be detected for the first time at a sensitivity comparable to that of radioactive probes, but without the danger inherent in radioactivity. [7] Although some sequence specificity that might lead to inhomogeneous staining has been demonstrated for both TOTO and, to a lesser extent, YOYO, [8][9][10] these cyanines and some derivatives have been used as general DNA stains in numerous DNA detection and quantitation assays, [11] such as the polymerase chain reaction, [12,13] DNA staining and fragment sizing, [14][15][16][17][18] DNA damage detection, [19,20] flow cytometry, [16,21,22] evaluation of biological activity, [23,24] DNA imaging [25][26][27][28] and DNA photocleavage. [29][30][31] YO and TO have also been covalently linked to oligonucleotides and inserted into peptide nucleic acids constructs, which become fluorescent upon hybridisation of the light-up probe to a specific complementary strand.…”

mentioning

confidence: 99%

Structure–Fluorescence Contrast Relationship in Cyanine DNA Intercalators: Toward Rational Dye Design

Fürstenberg

Deligeorgiev

Gadjev

et al. 2007

Chemistry A European J

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The fluorescence enhancement mechanisms of a series of DNA stains of the oxazole yellow (YO) family have been investigated in detail using steady‐state and ultrafast time‐resolved fluorescence spectroscopy. The strong increase in the fluorescence quantum yield of these dyes upon DNA binding is shown to originate from the inhibition of two distinct processes: 1) isomerisation through large‐amplitude motion that non‐radiatively deactivates the excited state within a few picoseconds and 2) formation of weakly emitting H‐dimers. As the H‐dimers are not totally non‐fluorescent, their formation is less efficient than isomerisation as a fluorescent contrast mechanism. The propensity of the dyes to form H‐dimers and thus to reduce their fluorescence contrast upon DNA binding is shown to depend on several of their structural parameters, such as their monomeric (YO) or homodimeric (YOYO) nature, their substitution and their electric charge. Moreover, these parameters also have a substantial influence on the affinity of the dyes for DNA and on the ensuing sensitivity for DNA detection. The results give new insight into the development and optimisation of fluorescent DNA probes with the highest contrast.

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Current perspectives in protein array technology

Cited by 62 publications

References 81 publications

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

Forced Intercalation Probes (FIT Probes): Thiazole Orange as a Fluorescent Base in Peptide Nucleic Acids for Homogeneous Single‐Nucleotide‐Polymorphism Detection

Structure–Fluorescence Contrast Relationship in Cyanine DNA Intercalators: Toward Rational Dye Design

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