Current knowledge on isobutanol production withEscherichia coli,Bacillus subtilisandCorynebacterium glutamicum

Blombach, Bastian; Eikmanns, Bernhard J.

doi:10.4161/bbug.2.6.17845

Cited by 89 publications

(53 citation statements)

References 17 publications

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“…For both culture strategies, the management of the transition from fully aerobic to anaerobic culture conditions was suggested to be a key step to optimize succinate production [1]. This was confirmed by Blombach and Eikmanns [10] and Blombach et al [11] during the isobutanol production process by C. glutamicum or during the succinate production process by Escherichia coli [12]. In this last study, it was shown that the transition from fully aerobic to anaerobic phase had a strong impact on the ability of the microorganism to efficiently switch its enzymatic machinery from one metabolism to another.…”

Section: Introductionmentioning

confidence: 66%

Impact of gas–liquid mass transfer on organic acids production by Corynebacterium glutamicum in unbaffled shake flasks

Kaboré

Olmos

Blanchard

et al. 2015

Biochemical Engineering Journal

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Section: Introductionmentioning

confidence: 66%

Impact of gas–liquid mass transfer on organic acids production by Corynebacterium glutamicum in unbaffled shake flasks

Kaboré

Olmos

Blanchard

et al. 2015

Biochemical Engineering Journal

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“…The three α-KDCs we selected are KID1 , and ARO10 from S. cerevisiae , both of which have been implicated in the Ehrlich pathway 24 ; and Llkivd from L. lactis , which has been successfully used in several constructions of the isobutanol biosynthetic pathway 48 . Our selections for dehydrogenases are ADH7 from S. cerevisisae , which has also been implicated in fusel alcohol production in yeast 42 ; EcfucO from E. coli , which has been successfully used in the synthesis of heavy alcohols via the reverse beta-oxidation of fatty acids in bacteria 43 and LladhA RE1 from L. lactis , which has been engineered for increased affinity for isobutyraldehyde 26 .…”

Section: Methodsmentioning

confidence: 99%

Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols

2013

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Efforts to improve the production of a compound of interest in Saccharomyces cerevisiae have mainly involved engineering or overexpression of cytoplasmic enzymes. We show that targeted expression of metabolic pathways to mitochondria can increase production levels compared with expression of the same pathways in the cytoplasm. Compartmentalisation of the Ehrlich pathway into mitochondria increased isobutanol production by 260%, whereas overexpression of the same pathway in the cytoplasm only improved yields by 10%, compared with a strain overexpressing only the first three steps of the biosynthetic pathway. Subcellular fractionation of engineered strains reveals that targeting the enzymes of the Ehrlich pathway to the mitochondria achieves higher local enzyme concentrations. Other benefits of compartmentalization may include increased availability of intermediates, removing the need to transport intermediates out of the mitochondrion, and reducing the loss of intermediates to competing pathways.

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“…This introduced pathway possibly generates cofactor imbalance of the cell, which then limits the biosynthesis of isobutanol (Blombach & Eikmanns, 2011). For balancing cofactors in the cell, fdh from C. boidinii generating NADH and CO2 from formate or pntAB converting NADH + NADP + into NAD + + NADPH from E. coli were overexpressed, to regenerate NAD(P)H for biochemical production (Kabus, Georgi, Wendisch, & Bott, 2007;Madje, Schmolzer, Nidetzky, & Kratzer, 2012).…”

Section: Introduction Of Additional Nadph Regeneration System and Amentioning

confidence: 99%

Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered Escherichia coli

Song

Seo

Jeon

et al. 2018

Biotech & Bioengineering

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Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, its toxicity and low energy content make it hard to utilize as a sole carbon source for biochemical production. To increase acetate utilization and isobutanol production with engineered Escherichia coli, the feasibility of utilizing acetate and metabolic engineering was investigated. The expression of acs, pckA, and maeB increased isobutanol production by up to 26%, and the addition of TCA cycle intermediates indicated that the intermediates can enhance isobutanol production. For isobutanol production from acetate, acetate uptake rates and the NADPH pool were not limiting factors compared to glucose as a carbon source. This work represents the first approach to produce isobutanol from acetate with pyruvate flux optimization to extend the applicability of acetate. This technique suggests a strategy for biochemical production utilizing acetate as the sole carbon source.

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Current knowledge on isobutanol production withEscherichia coli,Bacillus subtilisandCorynebacterium glutamicum

Cited by 89 publications

References 17 publications

Impact of gas–liquid mass transfer on organic acids production by Corynebacterium glutamicum in unbaffled shake flasks

Impact of gas–liquid mass transfer on organic acids production by Corynebacterium glutamicum in unbaffled shake flasks

Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols

Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered Escherichia coli

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