Current and near-future technologies for antibiotic susceptibility testing and resistant bacteria detection

Dietvorst, Jiri; Vilaplana, Lluı̈sa; Uría, Naroa; Marco, Marı́a Pilar; Muñoz-Berbel, Xavier

doi:10.1016/j.trac.2020.115891

Cited by 71 publications

(46 citation statements)

References 54 publications

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“…Only cell lysates and tissue samples have similar complexity, and their analysis still lasts long. The reason is a longer time that is necessary for sample preparation, and the detection of residual antibiotics [224] in food is much less complex task than the detection of antibiotic‐resistant food pathogens [230]. Now, we are coming to the point where the detection with fast and reliable biosensors is not the problem [231], where the real bottleneck is on‐line sample preparation.…”

Section: New Trends In Detection Of Foodborne Pathogensmentioning

confidence: 99%

“…Cross‐reaction among the different tested pathogens was also not observed. An analysis of current and near‐future technologies for detection of bacteria [230] has the following result: presently, the shortest time necessary for sample preparation and the detection of pathogen bacteria is minimally about 2 h long (and more, see Ref. [235]); near future based technologies like Raman spectrometry [236] or microfluidic technologies [237] are also time consuming, again for the reason of sample pretreatment.…”

Section: New Trends In Detection Of Foodborne Pathogensmentioning

confidence: 99%

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Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Begić

Josić

2020

Electrophoresis

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Biofilm formation and extracellular microvesicles-The way of foodborne pathogens toward resistance Almost all known foodborne pathogens are able to form biofilms as one of the strategies for survival under harsh living conditions, to ward off the inhibition and the disinfection during food production, transport and storage, as well as during cleaning and sanitation of corresponding facilities. Biofilms are communities where microbial cells live under constant intracellular interaction and communication. Members of the biofilm community are embedded into extracellular matrix that contains polysaccharides, DNA, lipids, proteins, and small molecules that protect microorganisms and enable their intercellular communication under stress conditions. Membrane vesicles (MVs) are produced by both Gram positive and Gram negative bacteria. These lipid membrane-enveloped nanoparticles play an important role in biofilm genesis and in communication between different biofilm members. Furthermore, MVs are involved in other important steps of bacterial life like cell wall modeling, cellular division, and intercellular communication. They also carry toxins and virulence factors, as well as nucleic acids and different metabolites, and play a key role in host infections. After entering host cells, MVs can start many pathologic processes and cause serious harm and cell death. Prevention and inhibition of both biofilm formation and shedding of MVs by foodborne pathogens has a very important role in food production, storage, and food safety in general. Better knowledge of biofilm formation and maintaining, as well as the role of microbial vesicles in this process and in the process of host cells' infection is essential for food safety and prevention of both food spoilage and host infection.

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Section: New Trends In Detection Of Foodborne Pathogensmentioning

confidence: 99%

Section: New Trends In Detection Of Foodborne Pathogensmentioning

confidence: 99%

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Begić

Josić

2020

Electrophoresis

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“…Unexpectedly, they became even a bigger problem due to the changes in today's modern lifestyle and socioeconomical activities, which accelerates the spread of infection much faster around the world [ 2 ]. Due to this, many advances in detection and treatment of infectious disease have been studied and reported in the past decades, including developing different types of vaccines, innovative technologies, e.g., single-cell based studies, CRISPR technologies, RNA interference that help us to explore infectious disease more [ [3] , [4] , [5] , [6] ]. Moreover, CAR- and TCR-T cell-based therapies have been also investigated as new candidates [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

Kaya

Çetin²,

Azimzadeh

et al. 2021

Journal of Electroanalytical Chemistry

138

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“…Such diagnostic technologies are crucial for reducing empirical treatments for infectious diseases with broad-spectrum antibiotics, promoting evidence-based antibiotic prescriptions, enacting antibiotic stewardship, and facilitating resistance surveillance, thereby safeguarding existing antibiotics and minimizing the emergence of new multi-drug resistant microorganisms. [5][6][7][8][9][10][11][12] To this end, significant advances in molecular diagnostics have created rapid identification technologies that are becoming turnkey in clinical microbiology. AST technologies, on the other hand, currently remain reliant on time-consuming culturing and thus still require about 48 h after sample collection.…”

Section: Introductionmentioning

confidence: 99%

A Cascaded Droplet Microfluidic Platform Enables High-throughput Single Cell Antibiotic Susceptibility Testing at Scale

Zhang

Kaushik

Hsieh

et al. 2021

Preprint

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Single-cell antibiotic susceptibility testing (AST) offers a promising technology by achieving unprecedented rapid testing time; however, its potential for clinical use is marred by its limited capacity for performing AST with scalable antibiotic numbers and concentrations. To lift the one antibiotic condition per device restriction common in single-cell AST, we develop a cascaded droplet microfluidic platform that uses an assembly line design to enable scalable single-cell AST. Such scalability is achieved by executing bacteria/antibiotic mixing, single-cell encapsulation, incubation, and detection in a streamlined workflow, facilitating susceptibility testing of each new antibiotic condition in 2 min after a 90 min setup time. As a demonstration, we test 3 clinical isolates and 8 positive urine specimens against 15 antibiotic conditions for generating antiprograms in ~2 h and achieve 100% and 93.8% categorical agreement, respectively, compared to laboratory-based clinical microbiology reports which becomes available only after 48 h.

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Current and near-future technologies for antibiotic susceptibility testing and resistant bacteria detection

Cited by 71 publications

References 54 publications

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

A Cascaded Droplet Microfluidic Platform Enables High-throughput Single Cell Antibiotic Susceptibility Testing at Scale

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