Current advances in the algae-made biopharmaceuticals field

Rosales‐Mendoza, Sergio; Solís-Andrade, Karla I.; Márquez-Escobar, Verónica Araceli; González‐Ortega, Omar; Bañuelos-Hernández, Bernardo

doi:10.1080/14712598.2020.1739643

Cited by 43 publications

(40 citation statements)

References 116 publications

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“…In the context of using microalgae as a biopharmaceutical platform for the production of recombinant proteins dedicated to therapeutic applications in humans, it will be crucial to unravel the protein glycosylation pathways and then optimize them in order to mimic human-type N-and O-glycans through metabolic engineering (Dumontier et al, 2018;Barolo et al, 2020;Rosales-Mendoza et al, 2020). This represents an important challenge for the next decades.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, microalgae have been investigated as emerging industrial platforms for the production of high value-added biopharmaceuticals (Barolo et al, 2020;Dehghani et al, 2020;Rosales-Mendoza et al, 2020). Indeed, microalgae are easy and fast to grow, safe and cheap making them attractive expression systems for the production of therapeutic proteins (Hempel and Maier, 2016;Rosales-Mendoza et al, 2020). Nowadays, the most biological expression systems used for the production of recombinant proteins are bacteria, yeast and mammalian cells (Walsh, 2014).…”

Section: Introductionmentioning

confidence: 99%

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N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

et al. 2020

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The term microalga refers to various unicellular and photosynthetic organisms representing a polyphyletic group. It gathers numerous species, which can be found in cyanobacteria (i.e., Arthrospira) as well as in distinct eukaryotic groups, such as Chlorophytes (i.e., Chlamydomonas or Chlorella) and Heterokonts (i.e., diatoms). This phylogenetic diversity results in an extraordinary variety of metabolic pathways, offering large possibilities for the production of natural compounds like pigments or lipids that can explain the ever-growing interest of industrials for these organisms since the middle of the last century. More recently, several species have received particular attention as biofactories for the production of recombinant proteins. Indeed, microalgae are easy to grow, safe and cheap making them attractive alternatives as heterologous expression systems. In this last scope of applications, the glycosylation capacity of these organisms must be considered as this post-translational modification of proteins impacts their structural and biological features. Although these mechanisms are well known in various Eukaryotes like mammals, plants or insects, only a few studies have been undertaken for the investigation of the protein glycosylation in microalgae. Recently, significant progresses have been made especially regarding protein N-glycosylation, while O-glycosylation remain poorly known. This review aims at summarizing the recent data in order to assess the state-of-the art knowledge in glycosylation processing in microalgae.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

et al. 2020

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“…P. tricornutum is an unicellular Stramenopile believed to have arisen via a serial endosymbiotic event in which a red microalga were engulfed by a heterotroph (Moustafa et al, 2009;Bowler et al, 2010;Prihoda et al, 2012), thus generating specific genomic features and metabolic pathways (Bowler et al, 2008;Keeling and Palmer, 2008). Indeed, a recent investigation of P. tricornutum genome revealed that a total of 3,170 genes (26%) are unique and specific to this organism (Rastogi et al, 2018). P. tricornutum also harbors a combination of genes and metabolic pathways that belongs either to the plant or animal kingdoms (C4 photosynthetic pathway and urea cycle, for example) (Butler et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain

et al. 2021

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The diatom Phaeodactylum tricornutum is a marine unicellular microalga that exists under three main morphotypes: oval, fusiform, and triradiate. Previous works have demonstrated that the oval morphotype of P. tricornutum Pt3 strain presents specific metabolic features. Here, we compared the cellular organization of the main morphotypes of the diatom P. tricornutum Pt3 strain through transmission electron and advanced light microscopies. The three morphotypes share similarities including spectral characteristics of the plastid, the location of the nucleus, the organization of mitochondria around the plastid as well as the existence of both a F-actin cortex, and an intracellular network of F-actin. In contrast, compared to fusiform and triradiate cells, oval cells spontaneously release proteins more rapidly. In addition, comparison of whole transcriptomes of oval versus fusiform or triradiate cells revealed numerous differential expression of positive and negative regulators belonging to the complex dynamic secretory machinery. This study highlights the specificities occurring within the oval morphotype underlying that the oval cells secrete proteins more rapidly.

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“…It is conceivable that the mammalian immune response might have evolved in the absence of the need to recognize photosynthetic cells as foreign entities ( Wheeler et al, 2008 ). In addition, neither critical pathogen-associated molecular patterns compounds that are recognized by the native immune system (e.g., endotoxic LPS) nor toxic compounds are present in most of the photosynthetic microorganisms ( Durai et al, 2015 ; Rosales-Mendoza et al, 2020 ). This raises hopes for the feasibility of establishing a photosymbiotic relationship resembling those utilized by reef-building corals, flatworms and adult salamanders.…”

Section: Discussionmentioning

confidence: 99%

Photosymbiosis for Biomedical Applications

Chávez

Moellhoff

Schenck

et al. 2020

Front. Bioeng. Biotechnol.

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Without the sustained provision of adequate levels of oxygen by the cardiovascular system, the tissues of higher animals are incapable of maintaining normal metabolic activity, and hence cannot survive. The consequence of this evolutionarily suboptimal design is that humans are dependent on cardiovascular perfusion, and therefore highly susceptible to alterations in its normal function. However, hope may be at hand. “Photosynthetic strategies,” based on the recognition that photosynthesis is the source of all oxygen, offer a revolutionary and promising solution to pathologies related to tissue hypoxia. These approaches, which have been under development over the past 20 years, seek to harness photosynthetic microorganisms as a local and controllable source of oxygen to circumvent the need for blood perfusion to sustain tissue survival. To date, their applications extend from the in vitro creation of artificial human tissues to the photosynthetic maintenance of oxygen-deprived organs both in vivo and ex vivo , while their potential use in other medical approaches has just begun to be explored. This review provides an overview of the state of the art of photosynthetic technologies and its innovative applications, as well as an expert assessment of the major challenges and how they can be addressed.

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Current advances in the algae-made biopharmaceuticals field

Cited by 43 publications

References 116 publications

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain

Photosymbiosis for Biomedical Applications

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