Curcumin‐loaded biodegradable polyurethane scaffolds modified with gelatin using 3D printing technology for cartilage tissue engineering

Lee, Min Jeong; Kim, Sung Eun; Park, Juri; Ahn, Guk Young; Yun, Tae Hoon; Choi, Inseong; Kim, Soo Hyun; Choi, Sung Wook

doi:10.1002/pat.4740

Cited by 14 publications

(7 citation statements)

References 13 publications

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“…In order to determine the concentration of unencapsulated and released curcumin, samples collected were analysed for curcumin concentration using a UV–vis spectrophotometer (GENESYS 150, Thermo Scientific). Prior to analysis, a calibration curve of curcumin in ethanol was constructed at the maximum absorbance wavelength of curcumin (425 nm) [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations

Ballacchino

Weaver

Mathew

et al. 2021

IJMS

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Microfluidic technique has emerged as a promising tool for the production of stable and monodispersed nanoparticles (NPs). In particular, this work focuses on liposome production by microfluidics and on factors involved in determining liposome characteristics. Traditional fabrication techniques for microfluidic devices suffer from several disadvantages, such as multistep processing and expensive facilities. Three-dimensional printing (3DP) has been revolutionary for microfluidic device production, boasting facile and low-cost fabrication. In this study, microfluidic devices with innovative micromixing patterns were developed using fused deposition modelling (FDM) and liquid crystal display (LCD) printers. To date, this work is the first to study liposome production using LCD-printed microfluidic devices. The current study deals with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes with cholesterol (2:1) prepared using commercial and 3D-printed microfluidic devices. We evaluated the effect of microfluidic parameters, chip manufacturing, material, and channel design on liposomal formulation by analysing the size, PDI, and ζ-potential. Curcumin exhibits potent anticancer activity and it has been reported that curcumin-loaded liposomes formulated by microfluidics show enhanced encapsulation efficiency when compared with other reported systems. In this work, curcumal liposomes were produced using the developed microfluidic devices and particle sizing, ζ-potential, encapsulation efficiency, and in vitro release studies were performed at 37 °C.

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Section: Methodsmentioning

confidence: 99%

Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations

Ballacchino

Weaver

Mathew

et al. 2021

IJMS

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“…Additionally, combinations thereof may be used in order to obtain end-use-specific properties. Thus, hydrogels based on different natural polymers (alginate [ 10 , 11 ], gelatin [ 12 , 13 ], cellulose [ 14 , 15 ], collagen [ 16 , 17 , 18 ], fibrinogen [ 19 , 20 ], hyaluronic acid [ 21 , 22 , 23 ]) or synthetic polymers (such as polyacrylamide [ 24 , 25 ], polyurethane [ 26 , 27 , 28 , 29 ], poly-(ethylene glycol) [ 30 , 31 ]) have been explored for the development of biomaterial inks. Naturally sourced bioactive polymers demonstrate superior biofunctionality over synthetic ones.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Naturally Sourced Mineral Clays for the 3D Printing of Biopolymer-Based Nanocomposite Inks

et al. 2021

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The present study investigated the possibility of obtaining 3D printed composite constructs using biomaterial-based nanocomposite inks. The biopolymeric matrix consisted of methacrylated gelatin (GelMA). Several types of nanoclay were added as the inorganic component. Our aim was to investigate the influence of clay type on the rheological behavior of ink formulations and to determine the morphological and structural properties of the resulting crosslinked hydrogel-based nanomaterials. Moreover, through the inclusion of nanoclays, our goal was to improve the printability and shape fidelity of nanocomposite scaffolds. The viscosity of all ink formulations was greater in the presence of inorganic nanoparticles as shear thinning occurred with increased shear rate. Hydrogel nanocomposites presented predominantly elastic rather than viscous behavior as the materials were crosslinked which led to improved mechanical properties. The inclusion of nanoclays in the biopolymeric matrix limited hydrogel swelling due the physical barrier effect but also because of the supplementary crosslinks induced by the clay layers. The distribution of inorganic filler within the GelMA-based hydrogels led to higher porosities as a consequence of their interaction with the biopolymeric ink. The present study could be useful for the development of soft nanomaterials foreseen for the additive manufacturing of customized implants for tissue engineering.

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“…While levels of inflammation were not measured in vivo postimplantation, the use of mild natural compounds may certainly be helpful in forming bioprinted constructs that can manage inflammation without stressing the seeded cells. Lee et al (2019) outlined a possible role for curcumin in bioprinted cartilage constructs (viz. a gelatin-curcumin bioink) but did not elaborate on any anti-inflammatory properties observed in their work.…”

Section: Cell-based Bioprinting Of Intervertebral Discsmentioning

confidence: 99%

3D Bioprinted Implants for Cartilage Repair in Intervertebral Discs and Knee Menisci

Perera

Ivone

Natekin

et al. 2021

Front. Bioeng. Biotechnol.

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Cartilage defects pose a significant clinical challenge as they can lead to joint pain, swelling and stiffness, which reduces mobility and function thereby significantly affecting the quality of life of patients. More than 250,000 cartilage repair surgeries are performed in the United States every year. The current gold standard is the treatment of focal cartilage defects and bone damage with nonflexible metal or plastic prosthetics. However, these prosthetics are often made from hard and stiff materials that limits mobility and flexibility, and results in leaching of metal particles into the body, degeneration of adjacent soft bone tissues and possible failure of the implant with time. As a result, the patients may require revision surgeries to replace the worn implants or adjacent vertebrae. More recently, autograft – and allograft-based repair strategies have been studied, however these too are limited by donor site morbidity and the limited availability of tissues for surgery. There has been increasing interest in the past two decades in the area of cartilage tissue engineering where methods like 3D bioprinting may be implemented to generate functional constructs using a combination of cells, growth factors (GF) and biocompatible materials. 3D bioprinting allows for the modulation of mechanical properties of the developed constructs to maintain the required flexibility following implantation while also providing the stiffness needed to support body weight. In this review, we will provide a comprehensive overview of current advances in 3D bioprinting for cartilage tissue engineering for knee menisci and intervertebral disc repair. We will also discuss promising medical-grade materials and techniques that can be used for printing, and the future outlook of this emerging field.

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Curcumin‐loaded biodegradable polyurethane scaffolds modified with gelatin using 3D printing technology for cartilage tissue engineering

Cited by 14 publications

References 13 publications

Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations

Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations

Assessment of Naturally Sourced Mineral Clays for the 3D Printing of Biopolymer-Based Nanocomposite Inks

3D Bioprinted Implants for Cartilage Repair in Intervertebral Discs and Knee Menisci

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