Curcumin-encapsulated nanoparticles as innovative antimicrobial and wound healing agent

Abstract

Burn wounds are often complicated by bacterial infection, contributing to morbidity and mortality. Agents commonly used to treat burn wound infection are limited by toxicity, incomplete microbial coverage, inadequate penetration, and rising resistance. Curcumin is a naturally derived substance with innate antimicrobial and wound healing properties. Acting by multiple mechanisms, curcumin is less likely than current antibiotics to select for resistant bacteria. Curcumin's poor aqueous solubility and rapid degradation profile hinder usage; nanoparticle encapsulation overcomes this pitfall and enables extended topical delivery of curcumin. In this study, we synthesized and characterized curcumin nanoparticles (curc-np), which inhibited in vitro growth of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in dose-dependent fashion, and inhibited MRSA growth and enhanced wound healing in an in vivo murine wound model. Curc-np may represent a novel topical antimicrobial and wound healing adjuvant for infected burn wounds and other cutaneous injuries.

Development of curcumin loaded sodium hyaluronate immobilized vesicles (hyalurosomes) and their potential on skin inflammation and wound restoring

“…Furthermore, the preparation method and the technology used to obtain hyalurosomes enrich the knowledge on phospholipid vesicle assembling because they were produced using an one-step, environmentallyfriendly, easily reproducible and organic solvent-free method by mixing the drug and the two natural and highly biocompatible main components (phospholipids and sodium hyaluronate). On the contrary, development of other nanocarriers generally requires 1) a complex preparation method involving several steps, often expensive and difficult to be reproduced on a large-scale; 2) use of organic solvents or not-safe and not-cheap, components; 3) complex chemical synthesis finalized to modify the main components and often involving the use of often toxic reagents or solvents [27][28][29][30]. The developed method allowed us to obtain polymer immobilized nanovesicles with peculiar physico-chemical properties (stability, high viscosity, biocompatibility) and versatile biological M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 17 behaviour resulting in high delivery performances in both intact and damaged skin.…”

HbE/β-Thalassemia and Oxidative Stress: The Key to Pathophysiological Mechanisms and Novel Therapeutics

“…Some of the topical indications illustrate that these nanoparticles effectively penetrate the skin and release NO in a controlled sustained manner. Using the same protocol, curcumin-releasing nanoparticles have also been prepared (98) and used topically with clear indications of transdermal delivery, release, and efficacy (214). Experiments are underway to evaluate whether sustained systemic delivery of NO and or curcumin can be achieved through topical application of drug-releasing nanoparticles.…”

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

“…Both standardized and modified microdilution and macrodilution methods have been applied to the determination of MIC and MBC values to evaluate the antimicrobial susceptibility of several microorganisms to nanoparticles [76,109,[110][111][112][113][114][115][116]. Additionally, resazurin staining assay has been employed to determine the MIC.…”

“…Viable plate counts have been frequently performed according to the various protocols to assess the antimicrobial efficacy of nanoparticles against both planktonic and biofilm-growing bacteria [109,128,[110][111][112]116]. Samples can either be spread or pipetted on agar plates followed by overnight incubation and determination of the number of CFUs [116,111,[113][114][115]. Especially, when quantifying the biofilm bacteria, efficient disaggregation of samples is of great importance to avoid false positive results.…”