Culture protocols for horse embryos after ICSI: Effect of myo-inositol and time of media change

Brom-de-Luna, J.G.; Salgado, Renato M.; Felix, M.; Canesin, H.S.; Stefanovski, Darko; Diaw, Mouhamadou; Hinrichs, K.

doi:10.1016/j.anireprosci.2021.106819

Cited by 7 publications

(1 citation statement)

References 37 publications

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“…Further development of this embryological research model might provide yet stronger empirical evidence for, and insights into the mechanisms of, the highly positive effect of NGR1. The model may also prove useful in improving the efficiency of propagating porcine and other mammalian embryos not only with the aid of in vitro fertilization (IVF) of oocytes via penetration by single capacitated/hyperactivated spermatozoa (Demyda‐Peyrás et al, 2013; Huang et al, 2021) or via microsurgically assisted intracytoplasmic sperm injection (ICSI) (Brom‐de‐Luna et al, 2021; Li et al, 2014; Okada et al, 2022), but also with the aid of somatic cell nuclear transfer (SCNT)‐based cloning (Samiec et al, 2013, 2022; Skrzyszowska et al, 2006; Wiater et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Improving the developmental competences of porcine parthenogenetic embryos by Notoginsenoside R1‐induced enhancement of mitochondrial activity and alleviation of proapoptotic events

He,

Liu,

Wang

et al. 2023

Reprod Domestic Animals

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Notoginsenoside R1 (NGR1), derived from the Panax notoginseng root and rhizome, exhibits diverse pharmacological influences on the brain, neurons, and osteoblasts, such as antioxidant effects, mitochondrial function protection, energy metabolism regulation, and inhibition of oxygen radicals, apoptosis, and cellular autophagy. However, its effect on early porcine embryonic development remains unclear. Therefore, we investigated NGR1's effects on blastocyst quality, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial function, and embryonic development‐related gene expression in porcine embryos by introducing NGR1 during the in vitro culture (IVC) of early porcine embryos. Our results indicate that an addition of 1 μM NGR1 significantly increased glutathione (GSH) levels, blastocyst formation rate, and total cell number and proliferation capacity; decreased ROS levels and apoptosis rates in orphan‐activated porcine embryos; and improved intracellular mitochondrial distribution, enhanced membrane potential, and reduced autophagy. In addition, pluripotency‐related factor levels were elevated (NANOG and octamer‐binding transcription factor 4 [OCT4]), antioxidant‐related genes were upregulated (nuclear factor‐erythroid 2‐related factor 2 [NRF2]), and apoptosis‐ (caspase 3 [CAS3]) and autophagy‐related genes (light chain 3 [LC3B]) were downregulated. These results indicate that NGR1 can enhance early porcine embryonic development by protecting mitochondrial function.

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