Culture of Methanogenic Archaea from Human Colostrum and Milk

Togo, Amadou Hamidou; Grine, Ghiles; Khelaifia, Saber; Robert, C.; Brevaut, Véronique; Caputo, Aurélia; Baptiste, Emeline; Bonnet, M; Levasseur, Anthony; Drancourt, Michel; Million, Matthieu; Raoult, Didier

doi:10.1038/s41598-019-54759-x

Cited by 51 publications

(45 citation statements)

References 67 publications

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“…Presence of M. smithii in the human gut is a feature of healthy lean adults while there is a depletion of this species in obese adults (Le Chatelier et al, 2013;Goodrich et al, 2014). In this context, M. smithii was less frequently detected by either culture or PCR in the milk samples obtained from obese mothers in the study of Togo et al (2019c). The human gastrointestinal tract is colonized by M. smithii early in life (Grine et al, 2017), and colostrum and milk may represent relevant sources of methanogenic archaea.…”

Section: The Composition Of the Human Milk Microbiotamentioning

confidence: 99%

“…Some studies have identified archaeal sequences in human milk (Ward et al, 2013;Jimeńez et al, 2015;Togo et al, 2019c). In addition, Togo et al (2019b) were able to isolate Methanobrevibacter smithii from 3 colostrum and 5 milk samples out of a total of 20 samples while Methanobrevibacter oralis was cultured from one milk sample.…”

Section: The Composition Of the Human Milk Microbiotamentioning

confidence: 99%

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The Microbiota of the Human Mammary Ecosystem

Fernández

Pannaraj

Rautava

et al. 2020

Front. Cell. Infect. Microbiol.

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Human milk contains a dynamic and complex site-specific microbiome, which is not assembled in an aleatory way, formed by organized microbial consortia and networks. Presence of some genera, such as Staphylococcus, Streptococcus, Corynebacterium, Cutibacterium (formerly known as Propionibacterium), Lactobacillus, Lactococcus and Bifidobacterium, has been detected by both culture-dependent and culture-independent approaches. DNA from some gut-associated strict anaerobes has also been repeatedly found and some studies have revealed the presence of cells and/or nucleic acids from viruses, archaea, fungi and protozoa in human milk. Colostrum and milk microbes are transmitted to the infant and, therefore, they are among the first colonizers of the human gut. Still, the significance of human milk microbes in infant gut colonization remains an open question. Clinical studies trying to elucidate the question are confounded by the profound impact of non-microbial human milk components to intestinal microecology. Modifications in the microbiota of human milk may have biological consequences for infant colonization, metabolism, immune and neuroendocrine development, and for mammary health. However, the factors driving differences in the composition of the human milk microbiome remain poorly known. In addition to colostrum and milk, breast tissue in lactating and non-lactating women may also contain a microbiota, with implications in the pathogenesis of breast cancer and in some of the adverse outcomes associated with breast implants. This and other open issues, such as the origin of the human milk microbiome, and the current limitations and future prospects are addressed in this review.

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Section: The Composition Of the Human Milk Microbiotamentioning

confidence: 99%

Section: The Composition Of the Human Milk Microbiotamentioning

confidence: 99%

The Microbiota of the Human Mammary Ecosystem

Fernández

Pannaraj

Rautava

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Intriguingly, some recent reports have also detected the presence of Archaea in human milk or colostrum samples [ 19 ]. At present, the roles of those archaea in human milk are not well understood, and possibly some of them could be involved in weight regulation [ 66 , 67 , 68 ]. However, all these factors need to be further investigated, and it is of interest to further study the roles of Archaea in human milk and their transfer from human milk to the gut of breast-fed infants in our next study.…”

Section: Discussionmentioning

confidence: 99%

The Impact of the Postpartum “Doing-the-Month” Practice on Human Milk Microbiota: A Pilot Study in Taiwan

2020

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Human milk microorganisms could benefit the healthy development of the immune system in infants. In Asia, the practice of “doing-the-month” indicates a month-long period of postpartum recuperation for new mothers. This is composed of cultural practices, traditional beliefs, behavioral, dietary, and herbal therapies. In this pilot study, we evaluated the effect of “doing-the-month” on the human milk microbiota using a molecular approach. We collected two “doing-the-month” milk groups from randomly recruited mothers who had completed their “doing-the-month” program in either postpartum care center A (milk-PCA, n = 14) or postpartum care center B (milk-PCB, n = 27) for 20 to 30 days. As for the control group, milk samples were selected from postpartum mothers (milk-H, n = 46), who did not conduct the “doing-the-month” program. We found that the “doing-the-month” milk samples were associated with more diverse and unique milk microbiota and that these samples were also linked with more abundant Lactobacillus (milk-PCB) and prevalent Bifidobacteria (milk-PCA and milk-PCB). In addition, the milk samples from “doing-the-month” mothers could be enriched with more Archaea bacterial members, but the “non-doing-the-month” milk samples were enriched with more common skin-, oral-, and environmental-related bacterial members. This study highlights the impact maternal practices may have on the milk microbiome. More research is needed to investigate the effects this may have on infant immune health.

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“…Accordingly, methanogens gained interest in the clinical microbiology over the last years after methanogens have been detected by PCR-based methods and cultured from the gut microbiota (2,3); and their translocation in milk and urines has been further observed (4). Moreover, methanogens have been associated with dysbiosis such as in the case of vaginosis (5), urinary tract infections (6) and anaerobe abscesses of the oral cavity in the case of periodontitis and periimplantitis (7,8), in the case of refractory sinusitis (9), brain (10,11) and muscle (12).…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported that the one-day newborns exhibited culturable Methanobrevibacter smithii (M. smithii) in the gastric fluid (20), suggesting a perinatal source of acquisition. Accordingly, we reported that mother milk did contain culturable M. smithii and culturable Methanobrevibacter oralis (M. oralis) (4). Yet, it is unclear whether these one-day methanogens do persist along with the digestive tract of the newborns or whether this is just one of several waves of acquisition of methanogens along the first months of the life (20)(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Stool methanogens in intestine mammal species

Davoust

Drancourt

Grine

2020

Preprint

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Methanogens are being members of anaerobe microbiota of the digestive tract of both human and mammals. However, the sources, modes of acquisition and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the spectrum of animals which could be sources of methanogens for human, by exploring methanogen carriage in animals in contact with the general population or with some restricted populations; comparing the repertoire of animal methanogens with the one of human methanogens in order to question methanogens as zoonotic microorganisms. We used RT-PCR, PCR-sequencing and multispacer sequence typing to investigate the presence of methanogens in 407 fecal specimens collected from nine different mammalian species. We detected by RT-PCR, the presence of methanogen DNA in all mammals here investigated and none of the negative controls. We obtained by sequencing, seven different species of methanogens, of which three ( Methanobrevibacter smithii, Methanobrevibacter millerae and Methanomassiliicoccus luminyensis ) are known to be part of the methanogens present in the human digestive tract. We obtained 24 M. smithii by PCR-sequencing including 12/24 (50%) in pigs, 6/24 (25%) in dogs, 4/24 (16.66%) in cats, and 1/24 (4.16%) in both sheep and horses. Genotyping these 24 M. smithii revealed five different genotypes, all know in humans. Our results are fairly representative of the methanogen community present in the digestive tract of certain animals domesticated by humans and other future studies must be done to try to cultivate methanogens here detected by molecular biology to better understand the dynamics of methanogens in animals and also the likely acquisition of methanogens in humans through direct contact with these animals or through consumption of the meat and/or milk of certain animals, in particular cows.

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Culture of Methanogenic Archaea from Human Colostrum and Milk

Cited by 51 publications

References 67 publications

The Microbiota of the Human Mammary Ecosystem

The Microbiota of the Human Mammary Ecosystem

The Impact of the Postpartum “Doing-the-Month” Practice on Human Milk Microbiota: A Pilot Study in Taiwan

Stool methanogens in intestine mammal species

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