Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production

Abstract

For biomass production of Spirulina platensis as feedstock of fermentation, the culture characteristics of three typical mutants of 3-A10, 3-B2 and 4-B3 generated by atmospheric and room temperature plasmas (ARTP) mutagenesis were systematically studied by using CO2 aeration culture system and compared with the wild strain. The specific growth rate of wild strain in the pure air aeration culture system exhibited a 76.2% increase compared with static culture, while the specific growth rates of the 3-A10, 3-B2 and 4-B3 in pure air aeration culture system were increased by 114.4%, 95.9% and 88.2% compared with their static cultures. Compared with static culture, the carbohydrate contents of wild strain, 3-A10, 3-B2 and 4-B3 in pure air aeration culture system dropped plainly by 51.0%, 79.3%, 85.5% and 26.1%. Increase of CO2 concentration enhanced carbohydrate content and productivity. Based on the carbohydrate productivity, the optimal inlet of CO2 concentration in aeration culture was determined to be 12% (v/v). Under this condition, 3-B2 exhibited the highest carbohydrate content (30.7%), CO2 fixation rate (0.120gCO2·g(-1)·d(-1)) and higher growth rate (0.093 g L(-1)·d(-1)), while 3-A10 showed the highest growth rate (0.118 g L(-1)·d(-1)) and higher CO2 fixation rate (0.117gCO2·g(-1)·d(-1)) but low carbohydrate content (24.5%), and 4-B3 showed the highest chlorophyll (Chl) content (3.82 mg·g(-1)). The most outstanding mutant by static culture in terms of growth rate and carbohydrate productivity (3-B2), was also demonstrated by CO2 aeration culture system. This study revealed that the ARTP mutagenesis could generate the S. platensis mutants suitable for CO2 aeration culture aiming at biomass production.

Enhancing biomass and lipid productivity of a green microalga Parachlorella kessleri for biodiesel production using rapid mutation of atmospheric and room temperature plasma

“…On the other hand, strain M5 showed the highest carotenoid content (2.63 µg/mgDW). These results were comparable with the findings of earlier studies that reported a ~ twofold increase in the total Chl of Spirulina platensis by applying ARTP [ 20 , 34 ]. In contrast, the chlorophyll content and fluorescence of Chlamydomonas reinhardtii were reported to decrease significantly after ARTP treatment [ 19 ].…”

“…The change in the content of cell components has been previously confirmed by ARTP mutagenesis [ 12 , 20 ]. In the present work, the M8 strain showed low carbohydrate content but a significant pigment, protein and lipid accumulation.…”

“…ARTP mutagenesis technique was also reported to increase growth rate and lipid accumulation in a dinoflagellate, Crypthecodinium cohnii [ 16 ]. There was also an improvement in growth rate, carbohydrate and chlorophyll contents, as well as CO 2 fixation rate in three mutants of Spirulina platensis [ 20 ].…”

Yield Enhancement of Recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP Mutagenesis-Screening and Medium Optimization

“…In 2010, Wang et al first successfully implemented the helium ARTP mutagenesis system to obtain mutants of Streptomyces avermitilis with high productivity of avermectins. Subsequently, the ARTP has been successfully employed to improve or decrease the different properties of various bacteria, fungi and microalgae (Jiang et al 2017;Liu et al 2017;Tan et al 2015), including the metabolite, biochemical and enzyme activities (Guo et al 2011;Lu et al 2011;Xu et al 2012;Zong et al 2012).…”

Comprehensive understanding of the mutant ‘giant’ Arthrospira platensis developed via ultraviolet mutagenesis

“…They play a key role in the global carbon/oxygen cycle ( Sánchez-Baracaldo et al., 2022 ) and are harnessed by diverse industries as sustainable feedstock for the production of high-value compounds ( Boutarfa et al., 2022 ; Santos-Merino et al., 2023 ). Moreover, they are considered ideal candidates for the development of next-generation biofuels and other biotechnologically relevant substances because of their potential for genetic manipulation and their ability to proliferate under diverse environmental conditions ( Tan et al., 2015 ; Patel et al., 2019 ).…”