2014
DOI: 10.1007/s10616-014-9807-z View full text |Buy / Rent full text
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Abstract: The purpose of this study was to establish methods for isolation, culture, expansion, and characterization of rat hair follicle stem cells (rHFSCs). Hair follicles were harvested from 1-week-old Sprague-Dawley rats and digested with dispase and collagenase IV. The bulge of the hair follicle was dissected under a microscope and cultured in Dulbecco's modified Eagle's medium/F12 supplemented with KnockOut™ Serum Replacement serum substitute, penicillin-streptomycin, L-glutamine, non-essential amino acids, epider… Show more

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“…In this work, we combined enzymatic digestion with microdissection to isolate gHFSCs, and differential adhesion to collagen IV to purify gHFSCs, voided disadvantages of methods mentioned above. This adhesion method was used in isolating rat HFSCs (Quan et al 2014), and we consider it could also be applied to isolate murine and human HFSCs.…”
Section: Discussionmentioning
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“…In this work, we combined enzymatic digestion with microdissection to isolate gHFSCs, and differential adhesion to collagen IV to purify gHFSCs, voided disadvantages of methods mentioned above. This adhesion method was used in isolating rat HFSCs (Quan et al 2014), and we consider it could also be applied to isolate murine and human HFSCs.…”
Section: Discussionmentioning
“…The small cell size and good adhesion ability of gHFSCs made digestion longer and more difficult compared with other types of cells. It has been reported that the HFSCs can be cultured in medium supplemented with 10 % FBS (Guo et al 2011;Quan et al 2014). In our study, most of the cells turned into spindle-like morphology when cultured with medium containing 10 % FBS.…”
Section: Discussionmentioning
“…HFSCs are abundant with no immunogenicity and are applicable to autologous transplantation; these cells are also a type of adult stem cell that can be easily obtained. The bulge region of hair follicles can be isolated by the tissue block adherence method (Amoh et al, 2009; Quan, Zheng, Ni, Xie, & Li, 2016). However, the isolation efficiency of HFSCs from a single bulge region is low.…”
Section: Discussionmentioning
“…Hundred isolated bulges were cut into fine parts, plated into collagen type І coated dish before cultivation. Coating of 60 mm dishes were done at room temperature for 1 h by diluted collagen type І (0.5 ml, 50 ml diluent) then solution was replaced by DMEM/F12 media containing 10% FBS for 2 h (Quan et al 2016 ). Pieces of bulges were placed in dishes and incubated in 37 °C and 5% CO 2 incubator to allow stacking within 1day.…”
Section: Methodsmentioning
“…Before culturing in osteogenic or adipogenic media, the MNCs, HF-MSCs (P3) and BM-MSCs (P3) were tested for viability by trypan blue stain. The cells were suspended at appropriate concentration of 1 × 10 5 /ml in α-MEM (Quan et al 2016 ). The mean of three repeated measurements were used.…”
Section: Methodsmentioning