Culturable hydrocarbonoclastic marine bacterial isolates from Indonesian seawater in the Lombok Strait and Indian Ocean

Abstract

A B S T R A C TPurpose: The study aims to isolate the culturable marine bacteria and to assess their potential as the bioremediation agent for petroleum hydrocarbons contamination in marine environment. Methods: Bacteria isolates were obtained by repetitive streaks to obtain purified bacteria on Zobell marine agar plates before further analysis and culture through direct visualization on agar plates. Identification were conducted using 16S rDNA sequence which are compared using NCBI BLAST and, combined with phenotypic and phylogenetic data. The potential use of the selected bacteria was tested by culturing them with two carbon sources i.e., glucose and crude oil.Result: Fifty-one culturable marine hydrocarbonoclastic bacteria were isolated from the Lombok Strait (LS-3, LS-13, LS-14, LS-15, and Indian Ocean (IO-1, IO-6, IO-8, IO-19, IO-24 and IO-25). Twelve isolates were found to degrade crude oil efficiently at a >2% concentration and to grow with crude oil as their sole carbon and energy source. These 12 strains belong to the genus Bacillus, which is well known to produce surface active agents, and the oil displacement assay indicated the production of these agents by these strains. Within the genera Bacillus, five species (Bacillus flexus, B. methylotrophicus, B. aquimaris, B. horikoshii, and B. thioparans) were represented by the 12 identified strains. Conclusion: Selected strains from the Lombok Strait and Indian Ocean were capable of degrading crude oil (2% v/ v) by 43.9-71.9% over 14 days. These results are important for marine bioremediation in Indonesia, which often faces risks of oil spill contamination and disaster.

Bioaugmentation of Native Fungi, an Efficient Strategy for the Bioremediation of an Aged Industrially Polluted Soil With Heavy Hydrocarbons

“…The sequence from the intense band 1 was identical to the type strain of Bacillus thioparans and, though less dominant than in the control soil, was still visible in most biostimulated and bioaugmented microcosm samples. This thiosulfate-oxidizing bacterium has previously been found to also degrade and grow with crude oil as its sole carbon and energy source ( Syakti et al, 2019 ). Another dominant ribotype from the control soil was distantly related to Immundisolibacter cernigliae as the closest known species (band 5, 90% sequence homology), but it was identical to an uncultured bacterium in the phylum Proteobacteria from a pilot-scale bioremediation process of a hydrocarbon-contaminated soil ( Militon et al, 2010 ).…”

Screening of Potential Biosurfactant Producing Bacteria from Tanjung Perak Port, Surabaya

“…The results of oil spreading showed that the 12 bacterial isolates dripped onto a layer of oil and water could form a clear zone. The diameter of the clear zone indicates the activity of surfactants, which is also known as oil displacement [18]. In contrast to the control, which did not form a clear area.…”

Valorization of Ketapang Leaves (Terminalia Catappa) as Immoblizing Agent for Hydrocarbon-Degrading Bacteria Dried Storage

“…The isolates were removed from the storage room and placed at room temperature for 1 hour to undergo the thawing process [15]. Furthermore, bacterial rejuvenation using the streak plate method on agar media [3].…”

“…The high activity of maritime shipping in Indonesia makes Indonesian waters vulnerable to marine pollution due to oil spills [2]. Oil waste contains hazardous compounds such as aliphatic hydrocarbon and polycyclic aromatic hydrocarbons [3]. These compounds pose a risk to marine animals and human health because they are carcinogenic [4].…”