Cultivable bacterial diversity associated with bromeliad roots from ironstone outcrops in central Brazil

Viana, Thianny Fernanda Carrelo; Campelo, A P S; Baldani, José Ivo; Fernandes‐Júnior, Paulo Ivan; Baldani, V. L. D.; Silva, W M; Paggi, Gecele Matos; Brasil, Marivaine Silva

doi:10.1590/1519-6984.224982

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…This strain was collected as an endophyte from Bromelia balansae Mez that occurs in ironstone outcrops in the Pantanal biome of Mato Grosso do Sul, Brazil [ 32 ]. This plant species is adapted to several stressful environmental factors, such as conditions of a high incidence of UV radiation, shallow soils with few nutrients, and periods of water scarcity [ 33 ]. In addition to all these peculiar characteristics, studies on endophytes from these sites and from the Pantanal are scarce, especially those centered on the characterization of secondary metabolites [ 34 , 35 , 36 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi

et al. 2023

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Metabolomics strategies are important tools to get holistic chemical information from a system, but they are scarcely applied to endophytic fungi to understand their chemical profiles of biosynthesized metabolites. Here Penicillium sp. was cultured using One Strain Many Compounds (OSMAC) conditions as a model system to demonstrate how this strategy can help in understanding metabolic profiles and determining bioactive metabolites with the application of metabolomics and statistical analyses, as well as molecular networking. Penicillium sp. was fermented in different culture media and the crude extracts from mycelial biomass (CEm) and broth (CEb) were obtained, evaluated against bacterial strains (Staphylococcus aureus and Pseudomonas aeruginosa), and the metabolomic profiles by LC-DAD-MS were obtained and chemometrics statistical analyses were applied. The CEm and CEb extracts presented different chemical profiles and antibacterial activities; the highest activities observed were against S. aureus from CEm (MIC = 16, 64, and 128 µg/mL). The antibacterial properties from the extracts were impacted for culture media from which the strain was fermented. From the Volcano plot analysis, it was possible to determine statistically the most relevant features for the antibacterial activity, which were also confirmed from biplots of PCA as strong features for the bioactive extracts. These compounds included 75 (13-oxoverruculogen isomer), 78 (austalide P acid), 87 (austalide L or W), 88 (helvamide), 92 (viridicatumtoxin A), 96 (austalide P), 101 (dihydroaustalide K), 106 (austalide k), 110 (spirohexaline), and 112 (pre-viridicatumtoxin). Thus, these features included diketopiperazines, meroterpenoids, and polyketides, such as indole alkaloids, austalides, and viridicatumtoxin A, a rare tetracycline.

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Section: Discussionmentioning

confidence: 99%

Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi

et al. 2023

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“…The most commonly characterized endophytic bacteria are Bacillus species (Lilley et al, 1996;Viana et al, 2020), with B. subtilis being particularly well known for its ability to promote plant growth (Poonguzhali et al, 2006) and to antagonize a range of plant pathogens, including Ceratocystis ulrni (Schreiber et al, 1988), Fusarium moniliforme (Hinton and Bacon, 1995), Xanthomonas oryzae (Lin et al, 2001), X. campestris pv. Campestris (Singh et al, 2010), Colletotrichum spp.…”

Section: Pcr-based Phylogenetic Analysesmentioning

confidence: 99%

Endophytic Bacillus subtilis P10 from Prunus cerasifera as a biocontrol agent against tomato Verticillium wilt

et al. 2023

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Endophytic bacteria serve key roles in the maintenance of plant health and growth. Few studies to date, however, have explored the antagonistic and plant growth-promoting (PGP) properties of Prunus cerasifera endophytes. To that end, we isolated endophytic bacteria from P. cerasifera tissue samples and used a dual culture plate assay to screen these microbes for antagonistic activity against Verticillium dahliae, Botryosphaeria dothidea, Fusarium oxysporum, F. graminearum, and F. moniliforme. Of the 36 strains of isolated bacteria, four (strains P1, P10, P16, and P20) exhibited antagonistic effects against all five model pathogens, and the P10 strain exhibited the strongest antagonistic to five pathogens. This P10 strain was then characterized in-depth via phenotypic assessments, physiological analyses, and 16s rDNA sequencing, revealing it to be a strain of Bacillus subtilis. Application of a P10 cell suspension (1×108 CFU/mL) significantly enhanced the seed germination and seedling growth of tomato in a greenhouse setting. This P10 strain further significantly suppressed tomato Verticillium wilt with much lower disease incidence and disease index scores being observed following P10 treatment relative to untreated plants in pot-based experiments. Tomato plants that had been treated with strain P10 also enhanced defense-related enzymes, peroxidase, superoxide dismutase, and catalase activity upon V. dahliae challenge relative to plants that had not been treated with this endophytic bacterium. The results revealed that the P10 bacterial strain has potential value as a biocontrol agent for use in the prevention of tomato Verticillium wilt.

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“…Previously, the 27 bacterial isolates used for this study were taxonomically and molecularly classi ed as Bacillus spp. (Viana et al 2020). The strains were previously isolated and classi ed by Viana et al (2020) and obtained from the Culture Collection of the Microbiology Laboratory of the Federal University of Mato Grosso do Sul, Campus Pantanal, Brazil.…”

Section: Origin Of Bacterial Strainsmentioning

confidence: 99%

“…(Viana et al 2020). The strains were previously isolated and classi ed by Viana et al (2020) and obtained from the Culture Collection of the Microbiology Laboratory of the Federal University of Mato Grosso do Sul, Campus Pantanal, Brazil. The bacteria were grown in tryptic soy agar (TSA) medium for 24 h at 30 °C to verify the purity before DNA extraction and the other assays.…”

Section: Origin Of Bacterial Strainsmentioning

confidence: 99%

Bacillus strains with potential for growth promotion and control of white mold in soybean

et al. 2022

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Plant growth-promoting rhizobacteria (PGPR) are known to stimulate the biocontrol of phytopathogenic bacteria and fungi, induce plant growth, and increase the yield of important economic crops. In this study, we evaluated in vivo, the ability of Bacillus strains to control soybean white mold disease and promote the growth of soybean plants under greenhouse conditions. Initially, 27 Bacillus strains were analyzed by PCR for the presence of genes encoding antimicrobial molecules, followed by in vitro tests of the positive strains against four phytopathogenic fungi, Sclerotinia sclerotiorum, Macrophomina phaseolina, Rhizoctonia solani, and Colletotrichum truncatum. The bacterial strains that returned positive results for antimicrobial genes were evaluated in vivo for their growth promoting capacity in soybean cultivars Potencia RR and M6210 IPRO, and white mold control in soybean plants. The results showed that eight strains presented the genes bamD, ituD, and fenF, while the bacAB gene was observed in 16 of all tested Bacillus strains. Brie y, the inoculation of the strain VBN02 in increased the fresh biomass of shoots and roots by 111.9% and 79.39% compared with the control. The greenhouse experiment showed that the inoculation of the strain VBN02 was the best treatment for increasing fresh shoot biomass of the soybean M6210 IPRO and Potencia RR cultivar in the single inoculation in relation to the control (111.9% and 103.57%, respectively). Co-inoculation of soybean inoculant and VBE01 was the superior treatment for increasing fresh shoot and root mass in both the cultivars. Two other strains, VBE05 and VBE01, reduced the disease progression of white mold by 39.1% and 37.5%, respectively. In conclusion, our results showed that the Bacillus strains have potential for biocontrol of white mold and for promoting the growth of soybean plants.

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Cultivable bacterial diversity associated with bromeliad roots from ironstone outcrops in central Brazil

Cited by 5 publications

References 23 publications

Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi

Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi

Endophytic Bacillus subtilis P10 from Prunus cerasifera as a biocontrol agent against tomato Verticillium wilt

Bacillus strains with potential for growth promotion and control of white mold in soybean

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