CuInSe/ZnS Core/Shell NIR Quantum Dots for Biomedical Imaging

Park, Jeaho; Dvoracek, C; Lee, Kwan H.; Galloway, Justin F.; Bhang, Hyo Eun C.; Pomper, Martin G.; Searson, Peter C.

doi:10.1002/smll.201101558

Cited by 100 publications

(71 citation statements)

References 26 publications

(29 reference statements)

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“…As seen from the spectrum, the presence of Au is from the Au sputtered sample grid used in the FESEM analysis. 42 The HRTEM image, particle size distribution from TEM (in powder phase), and DLS analysis (in solution phase) are shown in Figure 2C-E, respectively. From the Figure 2C, the particles are seem to be spherical and uniform but are fully agglomerated as similar to the result of FESEM (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Bwatanglang¹,

Mohammad²,

Yusof³

et al. 2016

IJN

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In this study, we synthesized a multifunctional nanoparticulate system with specific targeting, imaging, and drug delivering functionalities by following a three-step protocol that operates at room temperature and solely in aqueous media. The synthesis involves the encapsulation of luminescent Mn:ZnS quantum dots (QDs) with chitosan not only as a stabilizer in biological environment, but also to further provide active binding sites for the conjugation of other biomolecules. Folic acid was incorporated as targeting agent for the specific targeting of the nanocarrier toward the cells overexpressing folate receptors. Thus, the formed composite emits orange–red fluorescence around 600 nm and investigated to the highest intensity at Mn 2+ doping concentration of 15 at.% and relatively more stable at low acidic and low alkaline pH levels. The structural characteristics and optical properties were thoroughly analyzed by using Fourier transform infrared, X-ray diffraction, dynamic light scattering, ultraviolet-visible, and fluorescence spectroscopy. Further characterization was conducted using thermogravimetric analysis, high-resolution transmission electron microscopy, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence, and X-ray photoelectron spectroscopy. The cell viability and proliferation studies by means of MTT assay have demonstrated that the as-synthesized composites do not exhibit any toxicity toward the human breast cell line MCF-10 (noncancer) and the breast cancer cell lines (MCF-7 and MDA-MB-231) up to a 500 µg/mL concentration. The cellular uptake of the nanocomposites was assayed by confocal laser scanning microscope by taking advantage of the conjugated Mn:ZnS QDs as fluorescence makers. The result showed that the functionalization of the chitosan-encapsulated QDs with folic acid enhanced the internalization and binding affinity of the nanocarrier toward folate receptor-overexpressed cells. Therefore, we hypothesized that due to the nontoxic nature of the composite, the as-synthesized nanoparticulate system can be used as a promising candidate for theranostic applications, especially for a simultaneous targeted drug delivery and cellular imaging.

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Section: Resultsmentioning

confidence: 99%

Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Bwatanglang¹,

Mohammad²,

Yusof³

et al. 2016

IJN

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“…66 Recently, Park et al reported the synthesis of highly luminescent CuIn x Se y /ZnS core/ shell QDs (U = 0.6), with emission within the NIR biological window at 741 nm, a FWHM of 175 nm, and an average diameter of 5 nm. 67 With the exception of the large FWHM, these QDs are almost ideal for prospective in vivo applications. Some non-Cd QD materials (e.g., InP/ZnS, InGaP/ZnS) are currently available commercially.…”

Section: Quantum Dot Materialsmentioning

confidence: 99%

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

2013

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Semiconductor quantum dots (QDs) are brightly luminescent nanoparticles that have found numerous applications in bioanalysis and bioimaging. In this review, we highlight recent developments in these areas in the context of specific methods for fluorescence spectroscopy and imaging. Following a primer on the structure, properties, and biofunctionalization of QDs, we describe select examples of how QDs have been used in combination with steady-state or time-resolved spectroscopic techniques to develop a variety of assays, bioprobes, and biosensors that function via changes in QD photoluminescence intensity, polarization, or lifetime. Some special attention is paid to the use of Förster resonance energy transfer-type methods in bioanalysis, including those based on bioluminescence and chemiluminescence. Direct chemiluminescence, electrochemiluminescence, and charge transfer quenching are similarly discussed. We further describe the combination of QDs and flow cytometry, including traditional cellular analyses and spectrally encoded barcode-based assay technologies, before turning our attention to enhanced fluorescence techniques based on photonic crystals or plasmon coupling. Finally, we survey the use of QDs across different platforms for biological fluorescence imaging, including epifluorescence, confocal, and twophoton excitation microscopy; single particle tracking and fluorescence correlation spectroscopy; super-resolution imaging; near-field scanning optical microscopy; and fluorescence lifetime imaging microscopy. In each of the above-mentioned platforms, QDs provide the brightness needed for highly sensitive detection, the photostability needed for tracking dynamic processes, or the multiplexing capacity needed to elucidate complex systems. There is a clear synergy between advances in QD materials and spectroscopy and imaging techniques, as both must be applied in concert to achieve their full potential.

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“…However so far applications of NIR Q-dots to the clinical field have been hampered by the high toxicity of the NIR Q-dots such as Ag 2 S [17], InAs/InP/ZnSe [18] and CuInSe/ZnS [19]. Most of NIR Q-dots are synthesized via an organometallic precursor route in an organic solvent that is hazardous to the environment and to the health of the people [20].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

A near-infrared fluorescent bioassay for thrombin using aptamer-modified CuInS2 quantum dots

Lin

Pan

et al. 2015

Microchim Acta

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We describe a near-infrared (NIR) fluorescent thrombin assay using a thrombin-binding aptamer (TBA) and Zn(II)-activated CuInS 2 quantum dots (Qdots). The fluorescence of Zn(II)-activated Q-dots is quenched by the TBA via photoinduced electron transfer, but if thrombin is added, it will bind to TBA to form G-quadruplexes and the Q-dots are released. As a result, the fluorescence intensity of the system is restored. This effect was exploited to design an assay for thrombin whose calibration plot, under optimum conditions, is linear in the 0.034 to 102 nmol L −1 concentration range, with a 12 pmol L −1 detection limit. The method is fairly simple, fast, and due to its picomolar detection limits holds great potential in the diagnosis of diseases associated with coagulation abnormalities and certain kinds of cancer.

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CuInSe/ZnS Core/Shell NIR Quantum Dots for Biomedical Imaging

Cited by 100 publications

References 26 publications

Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Folic acid targeted Mn:ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

A near-infrared fluorescent bioassay for thrombin using aptamer-modified CuInS2 quantum dots

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