CTX-M1 ESBL-Producing
            <i>Klebsiella pneumoniae</i>
            subsp.
            <i>pneumoniae</i>
            Isolated from Cases of Bovine Mastitis

Locatelli, Clinio; Scaccabarozzi, L.; Pisoni, G.; Moroni, P.

doi:10.1128/jcm.00941-10

Cited by 77 publications

(29 citation statements)

References 15 publications

(4 reference statements)

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“…Our isolates contrast with the predominant isolates in dairy farms from other areas where E. coli and K. pneumoniae have been most frequently reported (Locatelli et al, 2010;Dahmen et al, 2013;Sudarwanto et al, 2015;Odenthal et al, 2016). However, the relevance of other bacteria of the Enterobacteriaceae family could be underestimated because studies have mainly focused on E. coli and K. pneumoniae (Smet et al, 2010;Schmid et al, 2013;Sudarwanto et al, 2015;Odenthal et al, 2016).…”

Section: Discussioncontrasting

confidence: 62%

Presencia de Enterobacteriaceae productoras de betalactamasa de espectro extendido (ESBL) en leche de tanque a granel de granjas lecheras bovinas en Antioquia, Colombia

Vásquez-Jaramillo

Ramı́rez

Akineden

et al. 2017

Rev Colomb Cienc Pecu

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Section: Discussioncontrasting

confidence: 62%

Presencia de Enterobacteriaceae productoras de betalactamasa de espectro extendido (ESBL) en leche de tanque a granel de granjas lecheras bovinas en Antioquia, Colombia

Vásquez-Jaramillo

Ramı́rez

Akineden

et al. 2017

Rev Colomb Cienc Pecu

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“…cause the most severe cases. [2][3][4] The search for the pathogenic mechanism of Klebsiella infections has identified a number of virulence factors in K. pneumoniae associated with primary pyogenic liver abscess which include capsular polysaccharides especially K1 or K2, lipopolysaccharides, hypermucoviscosity, adhesins, and iron acquisition systems. [5][6][7][8][9][10][11] However, very little is known about the presence and roles of virulence-associated genes products involved in the pathogenesis of K. pneumoniae associated with mastitis.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic, antimicrobial susceptibility profile and virulence factors ofKlebsiella pneumoniaeisolated from buffalo and cow mastitic milk

Osman

Hassan²,

Orabi

et al. 2014

Pathogens and Global Health

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Studies on the prevalence and virulence genes of Klebsiella mastitis pathogens in a buffalo population are undocumented. Also, the association of rmpA kfu, uge, magA, Aerobactin, K1 and K2 virulent factors with K. pneumoniae buffalo, and cow mastitis is unreported. The virulence of K. pneumoniae was evaluated through both phenotypic and molecular assays. In vivo virulence was assessed by the Vero cell cytotoxicity, suckling mouse assay and mice lethality test. Antimicrobial susceptibility was tested by disk diffusion method. The 45 K. pneumoniae isolates from buffalo (n510/232) and cow (n535/293) milk were isolated (45/525; 8.6%) and screened via PCR for seven virulence genes encoding uridine diphosphate galactose 4 epimerase encoding gene responsible for capsule and smooth lipopolysaccharide synthesis (uge), siderophores (kfu and aerobactin), protectines or invasins (rmpA and magA), and the capsule and hypermucoviscosity (K1 and K2). The most common virulence genes were rmpA, kfu, uge, and magA (77.8% each). Aerobactin and K1 genes were found at medium rates of 66.7% each and K2 (55.6%). The Vero cell cytotoxicity and LD (50) in mice were found in 100% of isolates. A multidrug resistance pattern was observed for 40% of the antimicrobials. The distribution of virulence profiles indicate a role of rmpA, kfu, uge, magA, Aerobactin, and K1 and K2 in pathogenicity of K. pneumoniae in udder infections and invasiveness, and constitutes a threat for vulnerable animals, even more if they are in combination with antibiotic resistance.

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“…Locatelli et al reported the isolation of a CTX-M-1-producing K. pneumoniae subsp. pneumoniae strain and a CTX-M-1-producing E. coli strain from cases of bovine mastitis in Italy (7,8). Recently, Geser et al also reported the isolation of an E. coli strain producing CTX-M-14/TEM-1 following analysis of 67 E. coli isolates from the milk of cattle with E. coli mastitis in Switzerland (4).…”

mentioning

confidence: 99%

Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom

Timofte

Maciuca

Evans

et al. 2014

Antimicrob Agents Chemother

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c Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum ␤-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids. E. coli isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried bla CTX-M-15 and bla TEM-1 , while K. pneumoniae subsp. pneumoniae isolates carried bla SHV-12 and bla TEM-1 . Conjugation experiments demonstrated that bla CTX-M-15 and bla TEM-1 were carried on a conjugative plasmid in E. coli, and PBRT identified this to be an IncI1 plasmid. The resistance genes were nontransferable in K. pneumoniae subsp. pneumoniae isolates. Moreover, in the E. coli isolates, an association of ISEcp1 and IS26 with bla CTX-M-15 was found where the IS26 element was inserted upstream of both ISEcp1 and the bla CTX-M promoter, a genetic arrangement highly similar to that described in some United Kingdom human isolates. We report the first cases in Europe of bovine mastitis due to E. coli CTX-M-15 and also of bovine mastitis due to K. pneumoniae subsp. pneumoniae SHV-12 ␤-lactamases in the United Kingdom. We also describe the genetic environment of bla CTX-M-15 and highlight the role that IncI1 plasmids may play in the spread and dissemination of ESBL genes, which have been described in both human and cattle isolates.

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CTX-M1 ESBL-Producing Klebsiella pneumoniae subsp. pneumoniae Isolated from Cases of Bovine Mastitis

Cited by 77 publications

References 15 publications

Presencia de Enterobacteriaceae productoras de betalactamasa de espectro extendido (ESBL) en leche de tanque a granel de granjas lecheras bovinas en Antioquia, Colombia

Presencia de Enterobacteriaceae productoras de betalactamasa de espectro extendido (ESBL) en leche de tanque a granel de granjas lecheras bovinas en Antioquia, Colombia

Phenotypic, antimicrobial susceptibility profile and virulence factors ofKlebsiella pneumoniaeisolated from buffalo and cow mastitic milk

Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom

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