CTX-M-15, OXA-30 and TEM-1-producing Escherichia coli in two Portuguese regions

Mendonça, Nuno; Louro, Deolinda; Castro, Ana; Diogo, José; Caniça, Manuela

doi:10.1093/jac/dkl062

Cited by 17 publications

(11 citation statements)

References 8 publications

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“…All 16 isolates were positive for bla TEM -and bla CTX-M -related genes and negative for bla OXA and bla SHV genes as assessed by PCR using previously described specific primers (13), and all isolates carried the ubiquitous ampC gene (13). Isoelectric focusing confirmed that all strains expressed both TEM-derived (pI of 5.4) and CTX-M-derived (pI of 8.0 and 8.9) enzymes ( Table 1).…”

Section: Sixteen Strains Ofmentioning

confidence: 84%

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

et al. 2006

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Sixteen strains of Escherichia coli isolated between January and June 2005 in a hospital in Algiers carry the ISEcp1 element and the TEM and either CTX-M-3 (n ‫؍‬ 3) or CTX-M-15 (n ‫؍‬ 13) ␤-lactamases. Fourteen of the isolates are multidrug resistant. Five isolates from the neonatal ward were indistinguishable by pulsed-field gel electrophoresis.CTX-M-type enzymes are the extended-spectrum ␤-lactamases (ESBL) most commonly produced by Enterobacteriaceae (4), and more than 55 CTX-M-type ␤-lactamases have been described (http://www.lahey.org/studies/webt.htm). Despite the prevalence of ESBL in Enterobacteriaceae, data from Algeria are scarce (although the prevalence has been reported to be 20 to 45%) (18). We investigated the phenotypic and ge-

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Section: Sixteen Strains Ofmentioning

confidence: 84%

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

et al. 2006

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“…They tend to mutate to extendedspectrum activity (Livermore, 1995). Epidemiological findings indicate that TEM-1 is the most common plasmidmediated β-lactamase between clinical Gram-negative bacteria (Bush and Jacoby, 1997;Sirot et al, 1997;Hoffmann et al, 2006;Mendonca et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Investigation of antibiotic resistance profile and TEM-type β-lactamase gene carriage of ampicillin-resistantEscherichia coli strains isolated from drinking water

et al. 2007

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Fifty-five ampicillin-resistant (Amp r ) Escherichia coli strains were isolated from 51 drinking water points in Rize region containing abundant fresh water sources in Turkey during the years 2000 to 2002 and from January to February 2004. The large number of organisms (nearly 57%) exhibited resistance to three or more antibiotics commonly used in human and veterinary medicine. These strains displayed a multiresistant phenotype. Nearly half of the strains (27%) expressed resistance to ceftazidime, but these strains were not an extended-spectrum β-lactamase-producer according to the results of double-disk synergy test. All isolates were then screened for the carriage of TEM-type β-lactamase gene (bla TEM ) by polymerase chain reaction. TEM-type β-lactamase genes were found in six (11%) isolates. Sequence analysis showed TEM-1 type genes. However, isoelectric focusing analysis did not confirm the production of TEM-1 type β-lactamase except for one strain. Conjugation experiments showed that resistance to ampicillin, tetracycline or trimethoprim/sulfamethoxazole was transferable in six (11%) isolates. Emergence of transferable antibiotic resistance and bla TEM-1 gene in E. coli strains from public drinking waters possesses a significant public health risk.

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“…recovered from healthy and sick children's [11 -16]. Nevertheless, in Portugal, to our knowledge, there are not studies on healthy children, but only performed on clinical human samples [17,18] and healthy humans [19,20].…”

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confidence: 99%

Detection of antibiotic resistant E. coli and Enterococcus spp. in stool of healthy growing children in Portugal

Barreto

Guimarães

Radhouani

et al. 2009

J. Basic Microbiol.

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From stool specimens of 118 healthy children's (1-14 years) in Portugal 92 E. coli and 101 Enterococcu s spp. strains have been isolated. Almost half (40.2%) of the E. coli isolates were resistant to ampicillin, 25.0% were resistant to tetracycline and 26.1% were resistant to streptomycin. Resistance genes detected by specific PCR included bla(TEM) and/or bla(SHV) and/or bla(CTX-M) (33 of 37 ampicillin and/or cefotaxime resistant isolates), tet (A) and/or tet (B) (16 of 23 tetracycline-resistant isolates), aad A (19 of 24 streptomycin-resistant isolates), cml A (in the two chloramphenicol-resistant isolates), aac (3)-II with/without aac (3)-IV (in the four gentamicin-resistant isolates), sul 1 and/or sul 2 and/or sul 3 (in all trimethoprim/sulfamethoxazole resistant isolates). The majority of the resistant E. coli isolates (69.1%) belonged to phylogenetic group B2. Of the enterococci isolates E. faecium (n = 53), E. faecalis (n = 41), E. hirae (n = 4) and E. durans (n = 3) more than one-fourth (28.7%) of the isolates were resistant to tetracycline; 21.8% were resistant to erythromycin and 8.9% were resistant to kanamycin. Resistance genes detected by PCR in enterococci included aph (3)'-IIIa (in all kanamycin-resistant isolates), aac (6') (in all gentamicin-resistant isolates), tet (M) and/or tet (L) (26 of 29 tetracycline-resistant isolates), erm (B) (17 of 22 erythromycin-resistant isolates). This survey showed that faecal bacteria such as E. coli and enterococci of healthy growing children's could be a reservoir of antimicrobial resistance genes.

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CTX-M-15, OXA-30 and TEM-1-producing Escherichia coli in two Portuguese regions

Cited by 17 publications

References 8 publications

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

Investigation of antibiotic resistance profile and TEM-type β-lactamase gene carriage of ampicillin-resistantEscherichia coli strains isolated from drinking water

Detection of antibiotic resistant E. coli and Enterococcus spp. in stool of healthy growing children in Portugal

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