Crystallization of citrate-stabilized amorphous calcium phosphate to nanocrystalline apatite: a surface-mediated transformation

Chatzipanagis, Konstantinos; Iafisco, Michele; Roncal-Herrero, Teresa; Bilton, Matthew; Tampieri, Anna; Kröger, Roland; Delgado-López, José Manuel

doi:10.1039/c6ce00521g

Cited by 67 publications

(78 citation statements)

References 33 publications

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“…This latter band was not observed in the spectra of samples Col/Ap and Eu-Col/Ap prepared in absence of citrate. It was observed that in all the Raman spectra of the samples prepared using citrate ions in the titrant solutions, the ν 1 PO 4 bands appeared at 951-953 cm -1 indicating the presence of ACP [42] in agreement with the XRD data. In addition it was also observed that the intensity ratio between the peak at 958-959 cm -1 (ν 1 PO 4 ) and that centered at 843-845 cm -1 (νC-C of citrate) is higher when the lowest amount of citrate was used, which confirm that citrate slowed down the growth of apatite and thus behaved as an inhibitor.…”

Section: Ftir and Raman Spectral Featuressupporting

confidence: 79%

Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions

Morales¹,

Fernández-Penas²,

Verdugo‐Escamilla³

et al. 2018

Crystals

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Synthetic nanostructured hybrid composites based on collagen and nanocrystalline apatites are interesting materials for the generation of scaffolds for bone tissue engineering. In this work, mineralized collagen fibrils were prepared in the presence of citrate and Eu 3+ . Citrate is an indispensable and essential structural/functional component of bone. Eu 3+ endows the mineralized fibrils of the necessary luminescent features to be potentially employed as a diagnostic tool in biomedical applications. The assembly and mineralization of collagen were performed by the neutralization method, which consists in adding dropwise a Ca(OH) 2 solution to a H 3 PO 4 solution containing the dispersed type I collagen until neutralization. In the absence of citrate, the resultant collagen fibrils were mineralized with nanocrystalline apatites. When citrate was added in the titrant solution in a Citrate/Ca molar ratio of 2 or 1, it acted as an inhibitor of the transformation of amorphous calcium phosphate (ACP) to nanocrystalline apatite. The addition of Eu 3+ and citrate in the same titrant solution lead to the formation of Eu 3+ -doped citrate-coated ACP/collagen fibrils. Interestingly, the relative luminescent intensity and luminescence lifetime of this latter composite were superior to those of Eu 3+ -doped apatite/collagen prepared in absence of citrate. The cytocompatibility tests, evaluated by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) colorimetric assay in a dose-dependent manner on GTL-16 human gastric carcinoma cells, on MG-63 human osteosarcoma cells and on the m17.ASC, a spontaneously immortalized mouse mesenchymal stem cell clone from subcutaneous adipose tissue, show that, in general, all samples are highly cytocompatible.

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Section: Ftir and Raman Spectral Featuressupporting

confidence: 79%

Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions

Morales¹,

Fernández-Penas²,

Verdugo‐Escamilla³

et al. 2018

Crystals

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“…For CaP068, CaP078, and CaP088, the Raman bands associated with crystalline CaP (CaHPO 4 , monetite) were distinct and clearly observed. In addition to the ν 1 band at around~987 cm −1 , the ν 2 (~380-430 cm −1 ), ν 3 (at around~1092 and 1129 cm −1 ), and ν 4 (~540-610 cm −1 ) bands were clearly observed for these samples [36][37][38]. The Raman bands marked with asterisk (*) could be attributed to the small amount of calcite in CaP058, CaP068 and CaP078, which was coincident with the PXRD results ( Figure 1b).…”

Section: Synthesis and Characterization Of Acps And Capsmentioning

confidence: 80%

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

et al. 2019

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Amorphous calcium phosphate (ACP) has shown significant effects on the biomineralization and promising applications in bio-medicine. However, the limited stability and porosity of ACP material restrict its practical applications. A storage stable highly porous ACP with Brunauer–Emmett–Teller surface area of over 400 m2/g was synthesized by introducing phosphoric acid to a methanol suspension containing amorphous calcium carbonate nanoparticles. Electron microscopy revealed that the porous ACP was constructed with aggregated ACP nanoparticles with dimensions of several nanometers. Large angle X-ray scattering revealed a short-range atomic order of <20 Å in the ACP nanoparticles. The synthesized ACP demonstrated long-term stability and did not crystallize even after storage for over 14 months in air. The stability of the ACP in water and an α-MEM cell culture medium were also examined. The stability of ACP could be tuned by adjusting its chemical composition. The ACP synthesized in this work was cytocompatible and acted as drug carriers for the bisphosphonate drug alendronate (AL) in vitro. AL-loaded ACP released ~25% of the loaded AL in the first 22 days. These properties make ACP a promising candidate material for potential application in biomedical fields such as drug delivery and bone healing.

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“…The XRD pattern of the ACP phase showed only two very broad and diffuse peaks, which is typical for a pattern lacking periodic LRO [175]. FT-IR and Raman methods use several bands in different vibration domains of PO 4 groups in the phosphate apatite to distinguish ACP [47,176]. Chatzipanagis et al used in situ time-resolved Raman spectroscopy to monitor amorphous phase structural evolution [176].…”

Section: Determination Of Amorphous Phases and Their Solubilitymentioning

confidence: 99%

“…Amorphous calcium carbonate (ACC) is the precursor phase for many invertebrate biomineralizations, such as in the formation of sea urchins [35,36], mollusk shells [23,[37][38][39], and coral skeletons [40]. The transformation of amorphous phases to crystalline phases (Table 1) can occur via several crystallization pathways [21,24,[41][42][43][44][45], such as surface-mediated heterogeneous nucleation [11,[46][47][48], dissolution/re-precipitation [49][50][51][52][53][54][55][56], and solid-solid phase transformation (structure reorganization) [19,33,[57][58][59][60][61][62]. The transformation mechanism and crystallization kinetics depend vitally on the detailed crystallization pathway and on the solution environment [52].…”

Section: Biomineralization Systemsmentioning

confidence: 99%

Amorphous Phase Mediated Crystallization: Fundamentals of Biomineralization

et al. 2018

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Many biomineralization systems start from transient amorphous precursor phases, but the exact crystallization pathways and mechanisms remain largely unknown. The study of a well-defined biomimetic crystallization system is key for elucidating the possible mechanisms of biomineralization and monitoring the detailed crystallization pathways. In this review, we focus on amorphous phase mediated crystallization (APMC) pathways and their crystallization mechanisms in bio-and biomimetic-mineralization systems. The fundamental questions of biomineralization as well as the advantages and limitations of biomimetic model systems are discussed. This review could provide a full landscape of APMC systems for biomineralization and inspire new experiments aimed at some unresolved issues for understanding biomineralization.

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Crystallization of citrate-stabilized amorphous calcium phosphate to nanocrystalline apatite: a surface-mediated transformation

Abstract: This is a repository copy of Crystallization of citrate-stabilized amorphous calcium phosphate to nanocrystalline apatite : a surface-mediated transformation.

Cited by 67 publications

References 33 publications

Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions

Bioinspired Mineralization of Type I Collagen Fibrils with Apatite in Presence of Citrate and Europium Ions

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

Amorphous Phase Mediated Crystallization: Fundamentals of Biomineralization

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