volume 65, issue 12, P1292-1295 2009
DOI: 10.1107/s1744309109045382
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Tatsuki Ebisawa, Akihiro Yamamura, Yasuhiro Kameda, Kou Hayakawa, Koji Nagata, Masaru Tanokura

Abstract: Monomeric Azami-Green (mAG) from the stony coral Galaxea fascicularis is the first monomeric green-emitting fluorescent protein that is not a derivative of Aequorea victoria green fluorescent protein (avGFP). mAG and avGFP are 27% identical in amino-acid sequence. Diffraction-quality crystals of recombinant mAG were obtained by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The mAG crystal diffracted X-rays to 2.20 A resolution on beamline AR-NW12A at the Photon Factory (Tsukuba, J…

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