Crystallization and preliminary X-ray analysis of stationary phase survival protein E (SurE) fromXylella fastidiosain two crystal forms

Abstract

The bacterium Xylella fastidiosa is a phytopathogenic organism that causes citrus variegated chlorosis, a disease which attacks economically important crops, mainly oranges. In this communication, the crystallization and preliminary X-ray crystallographic analysis of XfSurE, a survival protein E from X. fastidiosa, are reported. Data were collected for two crystal forms, I and II, to 1.93 and 2.9 Å resolution, respectively. Crystal form I belonged to space group C2, with unitcell parameters a = 172.36, b = 84.18, c = 87.24 Å , = = 90, = 96.59, whereas crystal form II belonged to space group C2, with unit-cell parameters a = 88.05, b = 81.26, c = 72.84 Å , = = 90, = 94.76 .

Conformational variability of the stationary phase survival protein E from Xylella fastidiosa revealed by X-ray crystallography, small-angle X-ray scattering studies, and normal mode analysis

“…Previously, Reis et al reported crystallization conditions and data collection of two crystal forms ( Xf SurE‐DS and Xf SurE‐TB). Thus, here we describe these for the remaining two forms.…”

“…Nucleotidases have some crystallographic structures deposited in the Protein Data Bank (PDB), distributed among several organisms: Thermotoga maritima ( Tm SurE): PDB ID's 1ILV, 1J9J, 1J9L, and 1J9K; Thermus thermophilus ( Tt SurE): PDB ID's 2E69, 2E6B, 2E6C, 2E6E, 2E6G, and 2E6H; Pyrobaculum aerophilum ( Pa SurE): PDB ID 1L5X; Aquifex aeolicus ( Aa SurE): PDB ID 2WQK; Salmonella typhimurium ( St SurE): PDB ID's 2V4N, 2V4O, 4G9O, and 4GAD, and Coxiella burnetii ( Cb SurE): PDB ID 3TY2 . Recently, our group reported the crystallization of Xf SurE and preliminary X‐ray crystallographic analyses . Two crystal forms were obtained; both belong to space group C 2, but with different number of monomers in the asymmetric unit …”

“…Recently, our group reported the crystallization of Xf SurE and preliminary X‐ray crystallographic analyses . Two crystal forms were obtained; both belong to space group C 2, but with different number of monomers in the asymmetric unit …”

“…14 Recently, our group reported the crystallization of XfSurE and preliminary X-ray crystallographic analyses. 15 Two crystal forms were obtained; both belong to space group C2, but with different number of monomers in the asymmetric unit. 15 Small-Angle X-ray Scattering (SAXS) studies provide relevant information about the protein folding in solution such as determination of the aggregation/oligomeric state, identification of flexible regions of the molecule, estimation of the radius of gyration, and maximum intramolecular distance as well as prediction of the three-dimensional protein shape.…”