Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein

Bujacz, G.; Pasternak, O.; Fujimoto, Yasuyuki; Hashimoto, Yuichi; Sikorski, M.; Jaskólski, Mariusz

doi:10.1107/s0907444902022916

Cited by 10 publications

(9 citation statements)

References 25 publications

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“…On such basis, it has been speculated that the putative PR10 binding site is compatible with the binding of nucleoside-like substrate [85]. The crystals of CSBP with zeatin have been obtained, however, the protein structure has not been solved yet [86].…”

Section: Cytokinin-binding Proteinsmentioning

confidence: 98%

Kinetin—A multiactive molecule

Barciszewski

Massino²,

Clark

2007

International Journal of Biological Macromolecules

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Section: Cytokinin-binding Proteinsmentioning

confidence: 98%

Kinetin—A multiactive molecule

Barciszewski

Massino²,

Clark

2007

International Journal of Biological Macromolecules

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“…In parallel, the practical use of sodium malonate as both a crystallization agent and a cryoprotectant for the PX domain of CISK has been reported (Xing & Xu, 2003). The use of sodium citrate in the crystallization and cryoprotection of plant pathogenesisrelated protein PR-10 and cytokinin-specific binding proteins has been reported (Bujacz et al, 2003;Pasternak et al, 2005Pasternak et al, , 2006Fernandes et al, 2008Fernandes et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%

Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme

Bujacz

Wrzesniewska

Bujacz

2010

Acta Crystallogr D Biol Cryst

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Currently, the great majority of the data that are used for solving macromolecular structures by X-ray crystallography are collected at cryogenic temperatures. Selection of a suitable cryoprotectant, which ensures crystal stability at low temperatures, is critical for the success of a particular diffraction experiment. The effectiveness of salts of organic acids as potential cryoprotective agents is presented in the following work. Sodium formate, acetate, malonate and citrate were tested, as were sodium potassium tartrate and acetate in the form of potassium and ammonium salts. For each salt investigated, the minimal concentration that was required for successful cryoprotection was determined over the pH range 4.5-9.5. The cryoprotective ability of these organic salts depends upon the number of carboxylic groups; the lowest concentration required for cryoprotection was observed at neutral pH. Case-study experiments conducted using the tetragonal form of hen egg-white lysozyme (HEWL) confirmed that salts of organic acids can successfully act as cryoprotective agents of protein crystals grown from high concentrations of inorganic salts. When crystals are grown from solutions containing a sufficient concentration of organic acid salts no additional cryoprotection is needed as the crystals can safely be frozen directly from the crystallizing buffers.

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“…While these proteins were first described as pathogenesis-related proteins, they probably have key roles in many plant developmental programs and biotic and abiotic stress responses (Hashimoto et al, 2004). Alternatively, they play a role in phytohormone homeostasis, as they were found to bind cytokinin and brassinosteroids and might thus provide a cellular mechanism to regulate the active cytokinin pool in the cell (Bujacz et al, 2003;Markovic-Housley et al, 2003). Since PR10-1 also is inducible by auxin (Poupard et al, 2001) and both auxin and cytokinin are involved in triggering embryo formation, PR10-1 might be a good candidate protein that mediates phytohormone responses in the cell.…”

Section: Comparison Of Proteomes Of Protoplast and Leaf Explant Culturesmentioning

confidence: 99%

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

et al. 2005

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The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from the cultures at different time points (2, 5, and 8 weeks), separated by two-dimensional gel electrophoresis, and detected by silver staining. More than 2,000 proteins could be reproducibly resolved and detected on each gel. Statistical analysis showed that 54 protein spots were significantly (P , 0.05) changed in expression (accumulation) during the 8 weeks of culture, and most of these spots were extracted from colloidal Coomassie-stained two-dimensional gel electrophoresis gels and were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry or liquid chromatography-tandem mass spectrometry analysis. Using a publicly available expressed sequence tag database and the Mascot search engine, we were able to identify 16 differentially expressed proteins. More than 60% of the differentially expressed protein spots had very different patterns of gene expression between 2HA and Jemalong during the 8 weeks of culture.Plants must coordinate the growth of root and shoot meristems to maintain an appropriate balance of root and shoot organs and to respond and adapt to various environmental conditions. This balance is achieved by an intermeristem coordination of growth and development of the plant and involves the interplay of several long-range signals (Wopereis et al., 2000;Jiang and Gresshoff, 2002;Searle et al., 2003). Somatic (or asexual) embryogenesis (SE) is the process whereby somatic cells differentiate into embryos and ultimately into plants via a series of characteristic morphological stages, particularly the later stages, which resemble the zygotic stages of development (Zimmerman, 1993;Schmidt et al., 1997). SE is the developmental restructuring of somatic cells toward the embryogenic pathway and forms the basis of cellular totipotency in higher plants (Nolan et al., 2003;Imin et al., 2004). Two experimental approaches are available to examine this process in detail: (1) leaf cells grown in culture from protoplasts to form calli and, subsequently, the generation of embryos and then the development of plants (Rose and Nolan, 1995); and (2) leaf explants, which form calli in culture, and the concomitant production of embryos and vascular tissues. Depending on the plant system, auxin and/or cytokinin are required to enable embryogenesis to occur in culture (Schmidt et al., 1997;Somleva et al., 2000;Baudino et al., 2001;Hecht et al., 2001). In Medicago truncatula (Australian barrel medic), Nolan et al. (2003) found that embryogenesis required both auxin and cytokinin addition, although some embryos could form on cytokinin alone.The first appearance of embryos from mesophyll protoplasts occurs...

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Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein

Cited by 10 publications

References 25 publications

Kinetin—A multiactive molecule

Kinetin—A multiactive molecule

Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

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