Crystal Structure of Species D Adenovirus Fiber Knobs and Their Sialic Acid Binding Sites

Burmeister, W.P.; Guilligay, Delphine; Cusack, Stephen; Wadell, Göran; Arnberg, Niklas

doi:10.1128/jvi.78.14.7727-7736.2004

Cited by 157 publications

(182 citation statements)

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“…Delphine Guilligay (EMBL Grenoble Outstation) generously provided a vector derived from pPROEX HTb (Invitrogen) containing a construct of the HAd37 fiber head (residues 177-365), an N-terminal His 6 tag, and a tobacco etch virus (TEV) protease cleavage site (12). A fragment coding for CAV-2 fiber head residues 358 -542 was generated by PCR using CAV-2 genomic DNA as template and cloned into a pQE vector (Qiagen) containing a noncleavable N-terminal His 6 tag.…”

Section: Methodsmentioning

confidence: 99%

“…The asymmetric unit contained one fiber head chain (194 residues, 21.7 kDa) and one chain of CAR D1 (138 residues, 15.3 kDa). Molecular replacement was performed with PHASER (53) using chain A of HAd37 fiber head (Protein Data Bank entry 1UXA) (12) and CAR D1 as found in complex with HAd12 fiber head (13) as models.…”

Section: Methodsmentioning

confidence: 99%

“…HAd3) and some species D viruses (e.g. HAd37) do not use CAR as a primary receptor in vivo but attach to CD46 (or MCP (membrane cofactor protein), a regulator of complement activation), sialic acids, and/or other primary receptors (12,(32)(33)(34)(35)(36). The short fibers of human enteric viruses HAd40 and HAd41 do not bind CAR either.…”

mentioning

confidence: 99%

“…The structures were solved to 2.3 and 1.5 Å resolution, respectively, significantly better than previous fiber head CAR complex structures. We also determined the structure of the CAV-2 fiber head alone at 1.5 Å resolution, which, together with the known structure of unliganded HAd37 fiber head (12), allows assessment of eventual conformational changes upon complex formation. Based on the structures, we analyzed the CAR-binding properties of wild type and mutant HAd37 and CAV-2 fiber heads using surface plasmon resonance (SPR).…”

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confidence: 99%

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Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor

Seiradake

Lortat‐Jacob

Billet

et al. 2006

Journal of Biological Chemistry

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Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and adenovirus receptor (CAR), although the binding residues are not strictly conserved. To understand this further, we determined the crystal structures of canine adenovirus serotype 2 (CAV-2) and the human adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and 1.5 Å resolution, respectively. Structure comparison with the HAd12 fiber head-CAR D1 complex showed that the overall topology of the interaction is conserved but that the interfaces differ in number and identity of interacting residues, shape complementarity, and degree of conformational adaptation. Using surface plasmon resonance, we characterized the binding affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved interactions can have a minor contribution, whereas serotype-specific interactions can be essential. These results are discussed in the light of virus evolution and design of adenovirus vectors for gene transfer. Adenoviruses (Ads)5 are nonenveloped icosahedral particles (70 -90 nm in diameter) containing double-stranded DNA genomes of 28 -42 kbp. They have been isolated from a wide variety of vertebrates, including mammals, birds, reptiles, amphibians, and fish (1). The ϳ50 human Ad serotypes (HAd) (divided into species A-F) lead to serotype-specific respiratory, ocular, and enteric infections that are usually self-limiting diseases in immunocompetent individuals. However, Ad infections are a significant cause of morbidity and mortality in newborns and immunosuppressed individuals (2). Notably, bone marrow and solid organ transplant patients who have severe lymphocytopenia are at the highest risk for Ad-induced disease (3). In addition, there are no drugs currently available that efficiently prevent or treat infections from all HAd serotypes.The major adenovirus capsid protein is the trimeric hexon, 240 of which form the 20 facets of the icosahedron. The 12 vertices are composed of the penton complex, which comprises the pentameric penton base and the externally projecting trimeric fiber. The fiber has a shaft of variable length with a terminal globular knob or head domain. In most cell types in vitro, the fiber head and penton base mediate attachment and entry, respectively. Crystal structures are available of the HAd2 and HAd5 hexons (4, 5); the HAd2 penton base (6); the fiber head domains of HAd2, HAd3, HAd5, HAd12, HAd37, HAd41 (short); and a construct of HAd2 fiber head plus part of its shaft (7-13) (reviewed in Ref. 14). In addition, there are recent high resolution cryoelectron microscopy reconstructions of the entire virus (15, 16). Based on similarities in overall capsid architecture and capsid protein structure, Adenoviridae probably share an ancient common ancestor with bacteriophages of the Tectiviridae family (17-19).The initial stages of adenoviral infection, cellular attachment, ...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor

Seiradake

Lortat‐Jacob

Billet

et al. 2006

Journal of Biological Chemistry

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“…A bioinformatic analysis was performed to assess this fiber's potential receptor-binding capabilities. Desmoglein 2-(DSG2), CD46-, sialic acid-and coxsackie and adenovirus receptor-(CAR) binding was analysed by comparing the HAdV-84 fiber sequence to those of HAdV-3 (NCBI Protein: YP_002213796) (Wang et al, 2013), HAdV-11 (AAN62521) (Persson et al, 2007), HAdV-19 (1UXB_A) and HAdV-37 (ABK59080) (Burmeister et al, 2004;Nilsson et al, 2011;Seiradake et al, 2006), respectively. Alignments with the above mentioned fiber knobs' aa sequences were conducted using the MAFFT G-INS-I method.…”

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confidence: 99%

The complete genome sequence of human adenovirus 84, a highly recombinant new Human mastadenovirus D type with a unique fiber gene

et al. 2017

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A novel human adenovirus was isolated from a pediatric case of acute respiratory disease in Panama City, Panama in 2011. The clinical isolate was initially identified as an intertypic recombinant based on hexon and fiber gene sequencing. Based on the analysis of its complete genome sequence, the novel complex recombinant Human mastadenovirus D (HAdV-D) strain was classified into a new HAdV type: HAdV-84, and it was designated Adenovirus D human/PAN/P309886/2011/84[P43H17F84]. HAdV-D types possess usually an ocular or gastrointestinal tropism, and respiratory association is scarcely reported. The virus has a novel fiber type, most closely related to, but still clearly distant from that of HAdV-36. The predicted fiber is hypothesised to bind sialic acid with lower affinity compared to HAdV-37. Bioinformatic analysis of the complete genomic sequence of HAdV-84 revealed multiple homologous recombination events and provided deeper insight into HAdV evolution.

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Lessons learned from adenovirus (1970–2019)

Nemerow

Flint

2019

FEBS Letters

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Animal viruses are well recognized for their ability to uncover fundamental cell and molecular processes, and adenovirus certainly provides a prime example. This review illustrates the lessons learned from studying adenovirus over the past five decades. We take a look back at the key studies of adenovirus structure and biophysical properties, which revealed the mechanisms of adenovirus association with antibody, cell receptor, and immune molecules that regulate infection. In addition, we discuss the critical contribution of studies of adenovirus gene expression to elucidation of fundamental reactions in pre‐mRNA processing and its regulation. Other pioneering studies furnished the first examples of protein‐primed initiation of DNA synthesis and viral small RNAs. As a nonenveloped virus, adenoviruses have furnished insights into the modes of virus attachment, entry, and penetration of host cells, and we discuss the diversity of cell receptors that support these processes, as well as membrane penetration. As a result of these extensive studies, adenovirus vectors were among the first to be developed for therapeutic applications. We highlight some of the early (unsuccessful) trials and the lessons learned from them.

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Crystal Structure of Species D Adenovirus Fiber Knobs and Their Sialic Acid Binding Sites

Cited by 157 publications

References 59 publications

Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor

Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor

The complete genome sequence of human adenovirus 84, a highly recombinant new Human mastadenovirus D type with a unique fiber gene

Lessons learned from adenovirus (1970–2019)

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