Crystal Structure and Regulation of the Citrus Pol III Repressor MAF1 by Auxin and Phosphorylation

Soprano, Adriana Santos; Giuseppe, P.O.; Shimo, Hugo; Lima, Tatiani B.; Batista, Fernanda Aparecida Heleno; Righetto, Germanna Lima; Pereira, José Geraldo de Carvalho; Granato, Daniela Campos; Nascimento, Andrey Fabricio Ziem; Gozzo, Fábio C.; Oliveira, Paulo Sérgio Lopes de; Figueira, Ana Carolina M.; Smetana, Juliana Helena Costa; Leme, Adriana Franco Paes; Murakami, Mário Tyago; Benedetti, Celso Eduardo

doi:10.1016/j.str.2017.07.004

Cited by 22 publications

(14 citation statements)

References 58 publications

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“…RNAPIII synthesizes various small noncoding RNAs that are essential for general biological activities. Dysregulation of the RNAPIII machinery results in multiple reported instances of reduced cellular and organismal growth (Dauwerse et al, 2011;Soprano et al, 2013Soprano et al, , 2017Borck et al, 2015;Johnson et al, 2016). However, the regulation of RNAPIII transcriptional activity remains poorly understood in plants.…”

Section: Discussionmentioning

confidence: 99%

The PLATZ Transcription Factor GL6 Affects Grain Length and Number in Rice

et al. 2019

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Grain size is one of the key determinants of grain yield. Although a number of genes that control grain size in rice (Oryza sativa) have been identified, the overall regulatory networks behind this process remain poorly understood. Here, we report the mapbased cloning and functional characterization of the quantitative trait locus GL6, which encodes a plant-specific plant AT-rich sequence-and zinc-binding transcription factor that regulates rice grain length and spikelet number. GL6 positively controls grain length by promoting cell proliferation in young panicles and grains. The null gl6 mutant possesses short grains, whereas overexpression of GL6 results in large grains and decreased grain number per panicle. We demonstrate that GL6 participates in RNA polymerase III transcription machinery by interacting with RNA polymerase III subunit C53 and transcription factor class C1 to regulate the expression of genes involved in rice grain development. Our findings reveal a further player involved in the regulation of rice grain size that may be exploited in future rice breeding.

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Section: Discussionmentioning

confidence: 99%

The PLATZ Transcription Factor GL6 Affects Grain Length and Number in Rice

et al. 2019

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“…The C-terminus of Maf1 is acidic in different species but its sequence is poorly conserved 23 . This region of the protein is predicted to be disordered and was removed for X-ray structure determination of both H. sapiens and C. sinensis Maf1 proteins 16,17 We also note that the regulatory internal region, that is disordered in our structure, and is subject to phosphorylation by TORC1, Sch9 and/or PKA 1,6 is located on the solvent-exposed side of Maf1 ( Figure S6). Maf1 can hence likely serve as a substrate to those kinases even in complex with Pol III, to allow rapid de-repression.…”

Section: Maf1-pol Iii-interface Mutants Impair Transcription Inhibitionmentioning

confidence: 95%

Structural basis of RNA polymerase III transcription repression by Maf1

Vorländer¹,

Baudin²,

Moir³

et al. 2019

Preprint

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Maf1 is a highly conserved central regulator of transcription by RNA polymerase III (Pol III), and Maf1 activity influences a wide range of phenotypes from metabolic efficiency to lifespan. Here, we present a 3.3 Å cryo-EM structure of yeast Maf1 bound to Pol III, which establishes how Maf1 achieves transcription repression. In the Maf1-bound state, Pol III elements that are involved in transcription initiation are sequestered, and the active site is sealed off due to ordering of the mobile C34 winged helix 2 domain. Specifically, the Maf1 binding site overlaps with the binding site of the Pol III transcription factor TFIIIB and DNA in the pre-initiation complex, rationalizing that binding of Maf1 and TFIIIB to Pol III are mutually exclusive. We validate our structure using variants of Maf1 with impaired transcription-inhibition activity. These results reveal the exact mechanism of Pol III inhibition by Maf1, and rationalize previous biochemical data.

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“…Despite these findings in yeast, the components and mechanisms that modulate RNAPIII transcription in plants are little understood. CsMAF1 from Citrus sinensis was the first characterized RNAPIII-interacting protein in plants, which can interact with the human RNAPIII and repress tRNA His synthesis in yeast [ 18 , 19 ], indicating that the functions of MAF1 proteins are evolutionally conserved across different kingdoms. Another example is UBL1, a putative RNA exonuclease belonging to the 2H phosphodiesterase superfamily, which possesses RNA exonuclease activity in vitro and is involved in biogenesis of snRNA U6.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the plant-specific PLATZ proteins in maize and identification of their general role in interaction with RNA polymerase III complex

Wang

Chen

et al. 2018

BMC Plant Biol

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BackgroundPLATZ proteins are a novel class of plant-specific zinc-dependent DNA-binding proteins that are classified as transcription factors (TFs). However, their common biochemical features and functions are poorly understood.ResultHere, we identified and cloned 17 PLATZ genes in the maize (Zea mays) genome. All ZmPLATZs were located in nuclei, consistent with their predicted role as TFs. However, none of ZmPLATZs was found to have intrinsic activation properties in yeast. Our recent work shows that FL3 (ZmPLATZ12) interacts with RPC53 and TFC1, two critical factors in the RNA polymerase III (RNAPIII) transcription complex. Using the yeast two-hybrid assay, we determined that seven other PLATZs interacted with both RPC53 and TFC1, whereas three had no protein-protein interaction with these two factors. The other six PLATZs interacted with either RPC53 or TFC1. These findings indicate that ZmPLATZ proteins are generally involved in the modulation of RNAPIII-mediated small non-coding RNA transcription. We also identified all of the PLATZ members in rice (Oryza sativa) and Arabidopsis thaliana and constructed a Maximum likelihood phylogenetic tree for ZmPLATZs. The resulting tree included 44 members and 5 subfamilies.ConclusionsThis study provides insight into understanding of the phylogenetic relationship, protein structure, expression pattern and cellular localization of PLATZs in maize. We identified nine and thirteen ZmPLATZs that have protein-protein interaction with RPC53 and TFC1 in the current study, respectively. Overall, the characterization and functional analysis of the PLATZ family in maize will pave the way to understanding RNAPIII-mediated regulation in plant development.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1443-x) contains supplementary material, which is available to authorized users.

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Crystal Structure and Regulation of the Citrus Pol III Repressor MAF1 by Auxin and Phosphorylation

Cited by 22 publications

References 58 publications

The PLATZ Transcription Factor GL6 Affects Grain Length and Number in Rice

The PLATZ Transcription Factor GL6 Affects Grain Length and Number in Rice

Structural basis of RNA polymerase III transcription repression by Maf1

Genome-wide analysis of the plant-specific PLATZ proteins in maize and identification of their general role in interaction with RNA polymerase III complex

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