Crystal growth and morphology of the nano-sized hydroxyapatite powders synthesized from CaHPO4·2H2O and CaCO3 by hydrolysis method

Shih, Wei-Jen; Chen, Yung-Feng; Wang, Moo–Chin; Hon, Min–Hsiung

doi:10.1016/j.jcrysgro.2004.06.023

Cited by 114 publications

(56 citation statements)

References 23 publications

(25 reference statements)

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“…29 The morphology and size of nHAP was analyzed using a transmission electron microscope (TEM) operated at 100 kV (JEOL JEM 2000EXII, JEOL, Tokyo, Japan). X-ray diffraction (XRD) diffractometer (Simens D5005) was used to examine the crystallographic phases of nHAP, with a monochromatic CuK radiation (wavelength =1.54056 Å).…”

Section: Synthesis and Characterization Of Nhap Nanoparticlesmentioning

confidence: 99%

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Lai

Shalumon

Chen

2015

IJN

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Incorporation of nanohydroxyapatite (nHAP) within a chitosan (CS)/silk fibroin (SF) nanofibrous membrane scaffold (NMS) may provide a favorable microenvironment that more closely mimics the natural bone tissue physiology and facilitates enhanced osteogensis of the implanted cell population. In this study, we prepared pristine CS/SF NMS, composite CS/SF/nHAP NMS containing intrafibrillar nHAP by in situ blending of 10% or 30% nHAP before the electrospinning step, and composite CS/SF/nHAP NMS containing extrafibrillar nHAP by depositing 30% nHAP through alternative soaking surface mineralization. We investigated the effect of the incorporation of HAP nanoparticles on the physicochemical properties of pristine and composite NMS. We confirmed the presence of ~30 nm nHAP in the composite nanofibrous membranes by thermogravimetry analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM), either embedded in or exposed on the nanofiber. Nonetheless, the alternative soaking surface mineralization method drastically influenced the mechanical properties of the NMS with 88% and 94% drop in Young’s modulus and ultimate maximum stress. Using in vitro cell culture experiments, we investigated the effects of nHAP content and location on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). The proliferation of hMSCs showed no significant difference among pristine and composite NMS. However, the extent of osteogenic differentiation of hMSCs was found to be positively correlated with the content of nHAP in the NMS, while its location within the nanofiber played a less significant role. In vivo experiments were carried out with hMSCs seeded in CS/SF/30%nHAP NMS prepared by in situ blending and subcutaneous implantation in nude mice. Micro-computed tomography images as well as histological and immunohistochemical analysis of the retrieved hMSCs/NMS construct 1 and 2 months postimplantation indicated that NMS had the potential for bone regeneration and can be suggested as a promising scaffold for bone tissue engineering.

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Section: Synthesis and Characterization Of Nhap Nanoparticlesmentioning

confidence: 99%

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Lai

Shalumon

Chen

2015

IJN

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“…Hydrothermal processes can be further classified into two categories: (i) hydrothermal conversion and (ii) hydrothermal crystal growth techniques. [128]) into HAp in aqueous media, usually with the aid of calcium, phosphate and/or alkaline sources to control the Ca/P ratio of HAp particles [129][130][131][132][133][134][135]. Hydrothermal conversions of poorly soluble calcium salts such as CaCO 3 (from coral [136][137][138], seashells [139,140], aragonite or calcite crystals [141,142]) and CaSO 4 · 2H 2 O [124] into HAp have also been studied, and the conversion mechanism-ion exchange, dissolution and recrystallization-has been discussed.…”

Section: Hydrothermal Processmentioning

confidence: 99%

Hydroxylapatite nanoparticles: fabrication methods and medical applications

Okada

Furuzono

2012

Science and Technology of Advanced Materials

126

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Hydroxylapatite (or hydroxyapatite, HAp) exhibits excellent biocompatibility with various kinds of cells and tissues, making it an ideal candidate for tissue engineering, orthopedic and dental applications. Nanosized materials offer improved performances compared with conventional materials due to their large surface-to-volume ratios. This review summarizes existing knowledge and recent progress in fabrication methods of nanosized (or nanostructured) HAp particles, as well as their recent applications in medical and dental fields. In section 1, we provide a brief overview of HAp and nanoparticles. In section 2, fabrication methods of HAp nanoparticles are described based on the particle formation mechanisms. Recent applications of HAp nanoparticles are summarized in section 3. The future perspectives in this active research area are given in section 4.

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“…[416] 2004 Citric acid sol-gel combustion process using calcium nitrate tetrahydrate, diammonium hydrogen orthophosphate and citric acid. [476] Nanodimensional powders of the stoichiometric HA of ~ 20 nm particle size were synthesized by hydrolysis of a mixture of DCPD and CaCO3 performed with 2.5 M aqueous solution of NaOH at 75 °C for 1 h. The only product synthesized was nanocrystalline HA and its crystallinity was improved with increasing annealing temperature [416]. Similar results were obtained in other studies [417,418].…”

Section: Yearmentioning

confidence: 99%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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Nano-sized particles and crystals play an important role in the formation of calcified tissues of various animals. For example, nano-sized and nanocrystalline calcium orthophosphates in the form of apatites of biological origin represent the basic inorganic building blocks of bones and teeth of mammals. Namely, according the recent developments in biomineralization, tens to hundreds nanodimensional crystals of a biological apatite are self-assembled into these complex structures. This process occurs under a strict control by bioorganic matrixes. Furthermore, both a greater viability and a better proliferation of various types of cells have been detected on smaller crystals of calcium orthophosphates. Thus, the nano-sized and nanocrystalline forms of calcium orthophosphates have a great potential to revolutionize the hard tissue-engineering field, starting from bone repair and augmentation to controlled drug delivery systems. This review reports on current state of the art and recent developments on the subject, starting from synthesis and characterization to biomedical and clinical applications. Furthermore, the review also discusses possible directions for future research and development.

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Crystal growth and morphology of the nano-sized hydroxyapatite powders synthesized from CaHPO4·2H2O and CaCO3 by hydrolysis method

Cited by 114 publications

References 23 publications

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Hydroxylapatite nanoparticles: fabrication methods and medical applications

Nanodimensional and Nanocrystalline Calcium Orthophosphates

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