Crystal distortion of monoclinic hen egg-white lysozyme crystals using X-ray digital topography

Existence of twisting in dislocation-free protein single crystals

“…After annealing, the holder was kept at 23 °C for 2 wk to obtain millimeter-sized crystals. Similarly, orthorhombic ( 34 ), monoclinic ( 35 ), and triclinic HEWL crystals ( 36 ) were also obtained. The crystallization conditions and crystal structures are summarized in SI Appendix , Tables S1 and S2 , respectively.…”

“…A series of digital X-ray topographs were obtained relative to the rotation of the sample crystal. The CCD camera outputs the sequential digital topographic images as 16-bit gray-scale TIFF files.To process the large amount of data in these images, we used the original computer software ( 26 , 35 , 37 ) to obtain mapping of the FWHM and peak position of the local rocking curves over the whole crystal. Further, the local rocking curve profiles were obtained from the reflected intensities in a selected circular area with a diameter of 96.75 μm (15 pixels) of the digital X-ray topographic image.…”

Identification of grown-in dislocations in protein crystals by digital X-ray topography

“…Digital X-ray topography can obtain not only sequential diffraction images but also local and global rocking curves, by reconstructing the diffraction intensity in the detector pixels. For various crystalline materials, the quality has been evaluated on the basis of the FWHM, the peak position, and the intensity of the rocking curves and the topographic images (Lü bbert et al, 2000(Lü bbert et al, , 2005Bellamy et al, 2000;Mikulík et al, 2003;Lovelace et al, 2004Lovelace et al, , 2006Lovelace & Borgstahl, 2010;Wako et al, 2012Wako et al, , 2014Stoupin et al, 2016). Digital X-ray topography is more useful for image analysis than the conventional X-ray film method.…”