2011
DOI: 10.1590/s0102-09352011000100011 View full text |Buy / Rent full text
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Abstract: The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions -1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted o… Show more

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“…Finally, COCs were washed (2 times) in BS free from sucrose for 5 min to remove the intracellular cryoprotectants effects (Hajarian et al, 2011), In vitro maturation:…”
Section: Vitrification Thawing and Evaluation Of Oocyte Viabilitymentioning
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“…Finally, COCs were washed (2 times) in BS free from sucrose for 5 min to remove the intracellular cryoprotectants effects (Hajarian et al, 2011), In vitro maturation:…”
Section: Vitrification Thawing and Evaluation Of Oocyte Viabilitymentioning
“…After storage for at least 2 weeks in LN2, all types of vitrified oocytes(immature or mature) were warmed by holding the OPS for 6 s in air and then agitating them in water bath at 20 °C for at least 10 s. The contents of OPS were expelled into Petri dish. To remove of intracellular cryoprotectants effects, oocytes were transferred in BM plus 0.25M sucrose for 5 min and then transferred to buffer solution (BS) plus 0.125M sucrose solution for 5 min and finally, the oocytes were washed twice in BS without sucrose for 5 min according to Hajarian et al (2011) with minor modifications.…”
Section: Thawing and Evaluation Of Oocyte Viabilitymentioning