Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability

Martín, E.; Regalado, J. J.; Perán-Quesada, R.; Encina, Carlos López

doi:10.1007/s11240-018-1392-y

Cited by 19 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are consistent with multiple previous reports that cryopreservation is generally considered a safe method for long-term germplasm conservation (e.g. Adu-Gyamfi et al 2010;Carmona-Martín et al 2018).…”

Section: Discussionsupporting

confidence: 93%

“…Nevertheless, negative effects on cryopreservation were anticipated. As increasing the ventilation is considered one way to combat hyperhydricity (Bhatia et al 2015), ventilated lids alone or in combination with a CO 2 -enriched environment were tested. We recorded a positive effect on plant quality using ventilated lids and even more so in plantlets grown with increased CO 2 supply.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Direct cryopreservation of winter-acclimated buds of Dracocephalum austriacum (Lamiaceae) from field material

Rasl

Schalk

Temsch

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

This study develops protocols for the micropropagation and cryopreservation of Dracocephalum austriacum (Lamiaceae). It is a perennial herbaceous plant that overwinters with ground-level sprouts and is classified as critically endangered in Europe. In vitro cultures were initiated from seeds on growth-regulator-free Murashige & Skoog (MS) medium after nicking the seed coat. Propagation via shoot culture was achieved on ½ MS medium with 1 µM benzyl adenine (BAP). Rooting on various indole-3-butyric acid (IBA)-media was not reliable, but the rooting success was 80% after 10 weeks on medium with 1 µM BAP. Two starting materials underwent cryopreservation: (1) shoot tips from cold-acclimated in vitro plantlets and (2) axillary buds from winter shoots from field plants. For the cryopreservation of in vitro shoots, plant vitrification solution (PVS)3 and incubation over ice yielded the best results (~ 34% regeneration success). However, regeneration using winter acclimated buds were 100, 76 and 30% for collections in December, February and March, respectively, using the same protocol. Moreover, the ploidy levels of cryopreserved plantlets were estimated using flow cytometry. The use of winteracclimated field material of temperate herbaceous plants or subshrubs has high potential as explant source for cryopreservation and calls for exploring this technique for other species.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Direct cryopreservation of winter-acclimated buds of Dracocephalum austriacum (Lamiaceae) from field material

Rasl

Schalk

Temsch

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…The ornamental plant analyses included Oncidium flexuosum by FCM [ 92 ], Chrysanthemum morifolium by SSR and FCM [ 82 ] and RAPD and ISSR [ 93 ], Argyranthemum by AFLP and ISSR [ 94 ], Torenia fournieri by FCM and ISSR [ 95 ], and Pleione bulbocodioides (protocorm-like bodies) by ISSR [ 96 ]. Similar results were obtained in vegetable crops, such as Phaseolus vulgariss (seeds) by SSR [ 97 ], Asparagus officinalis (rhizome buds) by EST-SSR and FCM [ 98 ], and Allium by SSR [ 81 ], AFLP and ISSR [ 99 ]. Examples of analyses of other crops included fruit trees, such as Musa (suck meristem) by SSR [ 100 ], Malus by FCM and ISSR [ 101 , 102 ], Vaccinium corymbosum by RAPD and ISSR [ 103 , 104 ], Vitis by RAPD and ISSR [ 17 ], and Actinidia chinensis by AFLP and ISSR [ 71 ], and medicinal species, such as Rabdosia rubescens by FCM and SRAP [ 105 ] and Bacopa monnieri by RAPD [ 106 ].…”

Section: Assessments Of Epigenetic and Genetic Integritysupporting

confidence: 67%

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Shukla

Ren

et al. 2021

Plants

View full text Add to dashboard Cite

Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

show abstract

“…Chen and Wang [ 48 ] developed a cryopreservation protocol for cell suspensions and protoplasts of carrot ( Daucus carota L.), while Fábián et al [ 49 ] used wheat ( Triticum aestivum L.) egg cells excised from the ovaries. Carmona-Martín et al [ 50 ], on the other hand, achieved 42–84% recovery of asparagus ( Asparagus officinalis L.) when rhizome buds were used. Root explants isolated from in vitro-grown plants, as well as adventitious and hairy root cultures, have been recently used in several cryopreservation protocols, including alfalfa ( Medicago truncatula Gaertn.)…”

Section: Plant Materials Used For Cryostoragementioning

confidence: 99%

“…germplasm cryopreserved with two PVS-2-based procedures, 100% genetic homogeneity was detected by ISSRs from Halaii and H 83-6179 cultivars, whereas 98.5% genetic stability was detected from NG 57-024 cultivar (Kaya and Souza 2017). Similarly, no ploidy changes were detected by flow cytometry (FCM) in asparagus cryopreserved with the encapsulation-dehydration technique [ 50 ]. Nonetheless, the potential effects of cryostorage of explants and seeds on subsequent plant growth in ex vitro conditions must be established before routine implementation in cryobanks, especially as genomic or phenotypic variation may result from tissue culture procedures commonly used in cryopreservation techniques.…”

Section: Stability and Functional Genomics Of Ln-derived Plant Materialsmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

View full text Add to dashboard Cite

Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

show abstract

Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability

Cited by 19 publications

References 35 publications

Direct cryopreservation of winter-acclimated buds of Dracocephalum austriacum (Lamiaceae) from field material

Direct cryopreservation of winter-acclimated buds of Dracocephalum austriacum (Lamiaceae) from field material

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Contact Info

Product

Resources

About