Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen

Isachenko, Evgenia; Isachenko, Vladimir; Rahimi, Gohar; Nawroth, Frank

doi:10.1016/s0301-2115(02)00465-7

Cited by 83 publications

(65 citation statements)

References 19 publications

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“…Until now, however, the production of live offspring from primordial and pre-antral follicles from cryopreserved/thawed ovarian tissue and cultured to mature in vitro has only been reported in immature mice (Liu et al 2001, O'Brien et al 2003, Hasegawa et al 2006. No live births have previously been reported using adult mouse ovaries, and although development of follicles to the antral stage has been observed in human ovarian tissue cultured in vitro (Isachenko et al 2003, Xu et al 2009, there have been no reports assessing the competency of the oocytes obtained from these in vitro grown follicles.…”

Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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“…Thus, the rapid cooling (1,500°C/min) in the presence of high concentrations of cryoprotectants avoids intracellular ice crystal formation. Vitrification has been used successfully with ovarian tissue with minimal changes in morphology [103,104], but when transplanted in mice and rats, diminished follicular development and fertility were observed compared with the case of fresh transplants. For examples, the number of antral follicles in cryopreserved grafts was smaller than in the fresh grafts [105].…”

Section: Vitrificationmentioning

confidence: 99%

Human oocyte and ovarian tissue cryopreservation and its application

Tao

Valle

2008

J Assist Reprod Genet

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Purpose To review the recent progress in human oocyte and ovarian tissue cryopreservation, and in the application of these two technologies for preserving female fertility of patients who are undergoing cancer treatment. Design The literature on human oocyte and ovarian tissue freezing was searched with PubMed. The scientific background, current developments and potential future applications of these two methods were reviewed.

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“…Until now, vitrification has been adopted to cryopreserve embryos [3,4], oocytes [5][6][7][8][9] and ovarian tissue [10][11][12][13]. Kuwayama reported that vitrification, compared with slow cooling, resulted in high survival rates for all stages of embryo development [14].…”

Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

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Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen

Cited by 83 publications

References 19 publications

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Human oocyte and ovarian tissue cryopreservation and its application

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

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