Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell® extender

Akhter, Shamim; Ansari, Muhammad Tayyab; Rakha, Bushra Allah; Andrabi, S. M. H.; Iqbal, Sajid; Ullah, Nemat

doi:10.1016/j.theriogenology.2010.04.024

Cited by 61 publications

(74 citation statements)

References 24 publications

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“…The first to find the AndroMed ® extender preferable were Aires et al (2003). In accord with the present findings, others confirmed this assessment for various ruminant species (Herold et al, 2003;Amirat et al, 2005;Janett et al, 2005;Akhter et al, 2010;Mohan and Atreja, 2014;Chelucci et al, 2015), although for sheep semen there are also controversial assessments (Fukui et al, 2008;Emamverdi et al, 2013). Many insemination organizations are hesitant to switch from the customary egg yolk to a phytoextender, because they are concerned that the latter may not be compatible with semen from all bulls and that, with the recently recommended substantial extension of the pre-freezing equilibration time, egg yolk seems to be indispensable.…”

Section: Discussionsupporting

confidence: 89%

Comparison of various semen extenders and addition of prostaglandin F2 on pregnancy rate in cows

Abdussamad

Detterer²,

Gauly

et al. 2016

animal

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This investigation comprises three trials. Trial 1 consists of an in vitro comparison of three semen extenders: two egg yolk based (customized Tris-egg yolk-glycerol and Triladyl ® ), the third (AndroMed ® ) soybean lecithin based. With regard to post-thaw motility, the phytoextender AndroMed ® proved to be superior (59 ± 3% v. 53 ± 2% and 53 ± 2%, P < 0.05). It had earlier been shown that addition of the commercial prostaglandin F 2α preparation Dinolytic ® before freezing compromises post-thaw motility; therefore, in Trial 2, Dinolytic ® was added after thawing. Frozen-thawed spermatozoa tolerated addition of Dinolytic ® at a concentration of 30% (v/v). In Trial 3, cows were inseminated using straws in which diluted semen and Dinolytic ® were frozen in the same straw, separated by an air bubble, so intermingling could only take place in the course of insemination. Pregnancy rates at Dinolytic ® dosages of 0%, 30% or 60% amounted to 44%, 41% and 56%, respectively ( P > 0.05), a result that encourages a large-scale field study, which is envisioned.Keywords: cattle, artificial insemination, spermatozoan motility, semen extender, prostaglandin F 2α ImplicationsPregnancy rates of well below 50% are common in most dairy operations. There is published evidence that addition of prostaglandin F 2α (PGF 2α ) to semen may improve conception rates, presumably by enhancing uterine contractility, and thus sperm transport to the site of fertilization. This could be particularly beneficial when inseminating with small sperm numbers, for example, sexed semen. The present study establishes preconditions (suitable semen extender, PGF 2α concentration tolerated by sperm cells and invention of a two-column straw artificial insemination technique) for implementation of a large-scale field investigation. IntroductionThe present study addresses the establishment of preconditions for a large-scale field trial aimed at verifying results reported by Gabriel et al. (2011), suggesting that intrauterine administration of prostaglandin F 2α (PGF 2α ) at the time of insemination improves the pregnancy rate in dairy cows. In that trial, i.m. injection of either Dinoprost (Dinolytic ® , Dinoprost-Trometamol; Zoetis, Berlin, Germany) or the synthetic PGF 2α analog cloprostenol was ineffective, and so was intrauterine infusion of cloprostenol. Intrauterine infusion of Dinoprost, however, brought about an increase in the proportion of cows falling pregnant. At slightly >220 cows/ treatment, the effect was not statistically significant (P = 0.12). Before initiating a new study involving substantially more animals per treatment, the practicability of the treatment was improved by incorporating Dinoprost into the insemination straw instead of having to administer it separately as practiced by Gabriel et al. (2011). The first intention of the present study was to compare various semen extenders on the basis of post-thaw motility. As a second step the concentration of Dinolytic ® that is tolerated by sperm cells was determined. Previous in vitro exp...

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Section: Discussionsupporting

confidence: 89%

Comparison of various semen extenders and addition of prostaglandin F2 on pregnancy rate in cows

Abdussamad

Detterer²,

Gauly

et al. 2016

animal

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“…Compared to post-thawed ejaculates sperm quality of Nili-Ravi buffaloes (Akhter et al, 2010;2011a), the quality and fertility of post-thawed epididymal sperm of spotted buffalo was not so much different. The delivery rate was 46.67% in TEY20 versus 40% in CEY20 in this study.…”

Section: Resultsmentioning

confidence: 75%

“…The delivery rate was 46.67% in TEY20 versus 40% in CEY20 in this study. The conception rate in river buffalo using post-thawed ejaculate sperm in different extenders was reported in widely range, i.e 44% in tris-egg yolk and 47% in Bioexcell (soya milk-based) extenders (Akhter et al, 2010), 41.5% in egg yolk-based and 56% in soya bean-based extenders (Akhter et al, 2011a), 41.5% in egg yolk-based and 53.5% in LDLs-based extenders (Akhter et al, 2011b).…”

Section: Resultsmentioning

confidence: 99%

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Yulnawati¹,

Maheshwari²,

Rizal³

et al. 2013

Med Pet

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Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo', as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity. Key words: artificial insemination, epididymal sperm, spotted buffalo ABSTRAKKerbau belang merupakan jenis kerbau unik yang ditemukan di Tana Toraja, Sulawesi Selatan. Populasi ternak ini menurun secara drastis karena tingginya laju pemotongan pada upacara adat Rambu Solo', serta keyakinan dalam adat yang tidak memperbolehkan kerbau belang jantan melakukan aktivitas reproduksi. Berdasarkan hal tersebut, studi ini dilakukan sebagai langkah awal proses konservasi dengan menggunakan cauda epididimis sebagai sumber sperma potensial. Studi ini menggunakan dua jenis bufer dalam bahan pengencer berbasis kuning telur, yaitu tris hidroxil amino metana (TEY20) dan trisodium sitrat dehidrat (CEY20), untuk menguji kualitas dan daya fertilitas sperma pasca-thawing dalam proses inseminasi buatan. Hasil yang diperoleh menunjukkan bahwa persentase motilitas progresif adalah sebesar 40% dan 39.17%, dengan persentase hidup mati sebesar 65.99% dan 63.26%, serta persentase membran plasma utuh sebesar 65.43% dan 63.03% dalam bahan pengencer TEY20 dan CEY20 secara berturut-turut. Dapat disimpulkan bahwa bahan pengencer berbasis tris dan sitrat memiliki kemampuan yang sama dalam mempertahankan kualitas dan daya fertilitas sperma epididimis kerbau belang pasca-thawing.

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“…Sperm plasma membrane integrity was assessed by hypo-osmotic swelling (HOS) 141 assay [26]. Solution for HOS assay consisted of 0.73g sodium citrate and 1.35g fructose 142 dissolved in 100 mL distilled water (osmotic pressure ~190 mOsmol kg …”

Section: Sperm Plasma Membrane Integrity 140mentioning

confidence: 99%

Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull semen

et al. 2017

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Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell® extender

Cited by 61 publications

References 24 publications

Comparison of various semen extenders and addition of prostaglandin F2 on pregnancy rate in cows

Comparison of various semen extenders and addition of prostaglandin F2 on pregnancy rate in cows

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull semen

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