Cryogenic preservation of Atlantic halibut sperm

Bolla, Sylvie; Holmefjord, Ivar; Refstie, Terje

doi:10.1016/0044-8486(87)90250-x

Cited by 33 publications

(22 citation statements)

References 6 publications

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“…Fertilization rate was higher after 8 h storage when the sperm was maintained at 2°C. Bolla et al (1987) reported no difference in fertilization success between fresh Atlantic halibut sperm and sperm diluted 1:3 with a saline extender and cryopreserved. Iiu (1991) evaluated both glycerol and dimethyl sulfoxide (DMSO) as protectants in an extender solution consisting of 800 mg/100 ml Nad, 500 mg/100 ml of NaHCO 3 , and 750 mg/100 ml lecithin as recommended by Horton and Ott (1976).…”

Section: Spawningmentioning

confidence: 96%

Culture of Atlantic halibut (Hippoglossus hippoglossus) and Pacific halibut (Hippoglossus stenolepis)

Stickney

Liu

1993

Reviews in Fisheries Science

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Section: Spawningmentioning

confidence: 96%

Culture of Atlantic halibut (Hippoglossus hippoglossus) and Pacific halibut (Hippoglossus stenolepis)

Stickney

Liu

1993

Reviews in Fisheries Science

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“…For the issues that make it difficult to get sperm such as: being protogynous hermaphrodites, inconsistent sexual maturation age and size, and gender imbalance, the semen cryopreservation is a successful method to solve the issues. Various diluents and cryoprotectants have been used to cryopreserve the sperms (Bolla et al, 1987; Gwo et al, 1991; Babiak et al, 1995). …”

Section: Discussionmentioning

confidence: 99%

Evaluation of Fertilizing Ability using Frozen Thawed Sperm in the Longtooth Grouper, Epinephelus bruneus

Oh¹,

Lee²,

Kang³

et al. 2013

Development &Amp; Reproduciton

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: This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: 99.5±0.8%, DMSO 7.5%: 99.5±0.7%, and DMSO 10.0%: 99.6±0.6%). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: 96.2±2.3%, DMSO 7.5%: 95.3±3.6%, 10.0%: 96.6±1.8%) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm (98.4%±0.5) and DMSO concentration level of 5.0% (97.8±0.1%) were not significantly different. However, with 7.5% (97.2±0.6%) and 10.0% DMSO concentrations (95.9±0.2%) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; 66.8±1.8%, HR: 82.0±12.9%) and 2 years (FR; 78.5±14.8%, HR: 79.3±0.6%) cryopreserved sperm were lower than control (FR; 100%, HR: 91.1±3.6%) and 2 days cryopreserved sperm (FR; 99.6±0.6%, HR: 96.6±1.8%). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.

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“…In flatfish species, successful cryopreservation of Atlantic halibut sperm was first reported by Bolla et al (1987). Since then, several studies reported the improved the quality of cryopreserved sperm as well as the technology needed for applications in industry (Babiak et al, 2006a; Ding et al, 2011).…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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Cryogenic preservation of Atlantic halibut sperm

Cited by 33 publications

References 6 publications

Culture of Atlantic halibut (Hippoglossus hippoglossus) and Pacific halibut (Hippoglossus stenolepis)

Culture of Atlantic halibut (Hippoglossus hippoglossus) and Pacific halibut (Hippoglossus stenolepis)

Evaluation of Fertilizing Ability using Frozen Thawed Sperm in the Longtooth Grouper, Epinephelus bruneus

Cryobanking of aquatic species

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