2021
DOI: 10.1681/asn.2020040501
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Abstract: BackgroundCrumbs2 is expressed at embryonic stages as well as in the retina, brain, and glomerular podocytes. Recent studies identified CRB2 mutations as a novel cause of steroid-resistant nephrotic syndrome (SRNS).MethodsTo study the function of Crb2 at the renal filtration barrier, mice lacking Crb2 exclusively in podocytes were generated. Gene expression and histologic studies as well as transmission and scanning electron microscopy were used to analyze these Crb2podKO knockout mice and their littermate con… Show more

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Cited by 10 publications
(7 citation statements)
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References 82 publications
(160 reference statements)
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“…Human immortalized podocytes, HEK293T, EA.hy926 and patient-derived urinary fibroblast cells were cultivated as previously described [ 10 , 11 , 24 , 25 ]. Podocytes were cultured at the permissive temperature of 33 °C and transfected using Lipofectamin 2000 (Thermo Fisher Scientific, Darmstadt, Germany) [ 26 ].…”
Section: Methodsmentioning
confidence: 99%
“…Human immortalized podocytes, HEK293T, EA.hy926 and patient-derived urinary fibroblast cells were cultivated as previously described [ 10 , 11 , 24 , 25 ]. Podocytes were cultured at the permissive temperature of 33 °C and transfected using Lipofectamin 2000 (Thermo Fisher Scientific, Darmstadt, Germany) [ 26 ].…”
Section: Methodsmentioning
confidence: 99%
“…Indeed, several proteins of the adherens-and tight junctions can also be found to be components of the slit diaphragm, e.g. ZO-1, Crumbs, PAR/aPKC-complex (Huber et al, 2003b;Hartleben et al, 2008;Hirose et al, 2009;Weavers et al, 2009;Hartleben et al, 2013;Itoh et al, 2014;Satoh et al, 2014;Hochapfel et al, 2017;Weide et al, 2017;Carrasco-Rando et al, 2019;Moller-Kerutt et al, 2021). Thus, it is likely that changes in PI(4,5)P2 and PI(3,4,5)P3 affect slit diaphragm formation and maintenance/stability like they affect adherens junctions/tight junctions in classical epithelia.…”
Section: Discussionmentioning
confidence: 99%
“…The quantitative real time RT-PCR was done as described earlier ( Weide et al, 2017 ; Möller-Kerutt et al, 2021 ). In brief, total RNA from mouse tissues was isolated using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich), according to the manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…The supernatants were mixed with 2× Laemmli buffer and incubated at 95°C for 5 min. The following steps were performed as described earlier ( Weide et al, 2017 ; Möller-Kerutt et al, 2021 ). In brief, equal volumes of lysates were fractioned on 10% SDS-PAGE gels at 150–200 V. In addition, a molecular size marker was loaded to identify the protein size.…”
Section: Methodsmentioning
confidence: 99%
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